Is flow cytometry better in counting malaria pigment-containing leukocytes compared to microscopy?
Abstract Background Detection of malaria pigment (or haemozoin; Hz)-containing leukocytes may have prognostic relevance in malaria; however, studies reported conflicting results, with microscopic counts suggestive of being inaccurate and imprecise. Methods Numbers of Hz-containing leukocytes from a...
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ftdoajarticles:oai:doaj.org/article:d23df4777bf94426aee7cda1d4a01f3b 2023-05-15T15:03:21+02:00 Is flow cytometry better in counting malaria pigment-containing leukocytes compared to microscopy? Längin Matthias Frita Rosangela Hänscheid Thomas Kremsner Peter G Grobusch Martin P 2009-11-01T00:00:00Z https://doi.org/10.1186/1475-2875-8-255 https://doaj.org/article/d23df4777bf94426aee7cda1d4a01f3b EN eng BMC http://www.malariajournal.com/content/8/1/255 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-8-255 1475-2875 https://doaj.org/article/d23df4777bf94426aee7cda1d4a01f3b Malaria Journal, Vol 8, Iss 1, p 255 (2009) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2009 ftdoajarticles https://doi.org/10.1186/1475-2875-8-255 2022-12-31T11:51:14Z Abstract Background Detection of malaria pigment (or haemozoin; Hz)-containing leukocytes may have prognostic relevance in malaria; however, studies reported conflicting results, with microscopic counts suggestive of being inaccurate and imprecise. Methods Numbers of Hz-containing leukocytes from a malaria patient obtained with a flow cytometer counting 50.000 gated events were compared with thin film microscopy as applied under field conditions. Results Flow cytometry identified 5.8% Hz-containing monocytes and 1.8% Hz-containing neutrophils. The microscopic examination yielded 10% and 13% of Hz-containing monocytes, as well as 0% and 0.5% of Hz-containing neutrophils for observers one and two, respectively. Conclusion Novel, robust and affordable cytometric methods should be evaluated in the field as they may assist in utilizing Hz-containing cells as clinically useful parameter. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 8 1 255 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Längin Matthias Frita Rosangela Hänscheid Thomas Kremsner Peter G Grobusch Martin P Is flow cytometry better in counting malaria pigment-containing leukocytes compared to microscopy? |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Detection of malaria pigment (or haemozoin; Hz)-containing leukocytes may have prognostic relevance in malaria; however, studies reported conflicting results, with microscopic counts suggestive of being inaccurate and imprecise. Methods Numbers of Hz-containing leukocytes from a malaria patient obtained with a flow cytometer counting 50.000 gated events were compared with thin film microscopy as applied under field conditions. Results Flow cytometry identified 5.8% Hz-containing monocytes and 1.8% Hz-containing neutrophils. The microscopic examination yielded 10% and 13% of Hz-containing monocytes, as well as 0% and 0.5% of Hz-containing neutrophils for observers one and two, respectively. Conclusion Novel, robust and affordable cytometric methods should be evaluated in the field as they may assist in utilizing Hz-containing cells as clinically useful parameter. |
format |
Article in Journal/Newspaper |
author |
Längin Matthias Frita Rosangela Hänscheid Thomas Kremsner Peter G Grobusch Martin P |
author_facet |
Längin Matthias Frita Rosangela Hänscheid Thomas Kremsner Peter G Grobusch Martin P |
author_sort |
Längin Matthias |
title |
Is flow cytometry better in counting malaria pigment-containing leukocytes compared to microscopy? |
title_short |
Is flow cytometry better in counting malaria pigment-containing leukocytes compared to microscopy? |
title_full |
Is flow cytometry better in counting malaria pigment-containing leukocytes compared to microscopy? |
title_fullStr |
Is flow cytometry better in counting malaria pigment-containing leukocytes compared to microscopy? |
title_full_unstemmed |
Is flow cytometry better in counting malaria pigment-containing leukocytes compared to microscopy? |
title_sort |
is flow cytometry better in counting malaria pigment-containing leukocytes compared to microscopy? |
publisher |
BMC |
publishDate |
2009 |
url |
https://doi.org/10.1186/1475-2875-8-255 https://doaj.org/article/d23df4777bf94426aee7cda1d4a01f3b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 8, Iss 1, p 255 (2009) |
op_relation |
http://www.malariajournal.com/content/8/1/255 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-8-255 1475-2875 https://doaj.org/article/d23df4777bf94426aee7cda1d4a01f3b |
op_doi |
https://doi.org/10.1186/1475-2875-8-255 |
container_title |
Malaria Journal |
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8 |
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1 |
container_start_page |
255 |
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1766335212498714624 |