Urinary tract infection: detection of Escherichia coli antigens in human urine with an ELIEDA immunoenzymatic assay
Escherichia coli is the most common causative agent of urinary tract infection (UTI), and diagnosing this infection usually relies on bacteriologic methods. Nevertheless, screening methods can be useful for a rapid presumptive diagnosis even though some of these screening methods have low sensitivit...
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Pan American Health Organization
1999
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ftdoajarticles:oai:doaj.org/article:d1fb94362d1b49bf8ea864839e91a3fa 2023-05-15T15:07:12+02:00 Urinary tract infection: detection of Escherichia coli antigens in human urine with an ELIEDA immunoenzymatic assay Myriam T. N. Campanhã Sumie Hoshino-Shimizu Marina Baquerizo Martinez 1999-08-01T00:00:00Z https://doi.org/10.1590/s1020-49891999000700002 https://doaj.org/article/d1fb94362d1b49bf8ea864839e91a3fa EN ES PT eng spa por Pan American Health Organization http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49891999000700002&lng=en&tlng=en https://doaj.org/toc/1020-4989 1020-4989 doi:10.1590/s1020-49891999000700002 https://doaj.org/article/d1fb94362d1b49bf8ea864839e91a3fa Revista Panamericana de Salud Pública, Vol 6, Iss 2, Pp 89-94 (1999) Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 1999 ftdoajarticles https://doi.org/10.1590/s1020-49891999000700002 2022-12-31T06:30:25Z Escherichia coli is the most common causative agent of urinary tract infection (UTI), and diagnosing this infection usually relies on bacteriologic methods. Nevertheless, screening methods can be useful for a rapid presumptive diagnosis even though some of these screening methods have low sensitivity or are expensive. To investigate a possible new alternative approach, an antigen-based immunoassay-enzyme-linked immunoelectrodiffusion assay (ELIEDA)-was standardized for screening for this bacterial infection. Combining counter immunoelectrophoresis with an immunoenzymatic assay, the ELIEDA requires concentrated urine specimens, a cellulose acetate membrane, polyclonal antibodies to E. coli raised in rabbits, and peroxidase-labeled sheep antibodies to rabbit immunoglobulin G (IgG). This ELIEDA technique was evaluated using 244 urine specimens, 76 of them with E. coli, 47 with heterologous bacteria, and 121 without bacteria. In comparison to bacteriologic methods, the sensitivity, specificity, and positive and negative predictive values for the ELIEDA were 93.4%, 98.2%, 95.9%, and 97.1%, respectively. The data obtained suggest that this assay is useful for routine diagnostic screening for UTI caused by E. coli. In addition, since the ELIEDA stained membranes can be stored, this assay makes retrospective studies possible. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista Panamericana de Salud Pública 6 2 89 94 |
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Directory of Open Access Journals: DOAJ Articles |
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English Spanish Portuguese |
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Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Myriam T. N. Campanhã Sumie Hoshino-Shimizu Marina Baquerizo Martinez Urinary tract infection: detection of Escherichia coli antigens in human urine with an ELIEDA immunoenzymatic assay |
topic_facet |
Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Escherichia coli is the most common causative agent of urinary tract infection (UTI), and diagnosing this infection usually relies on bacteriologic methods. Nevertheless, screening methods can be useful for a rapid presumptive diagnosis even though some of these screening methods have low sensitivity or are expensive. To investigate a possible new alternative approach, an antigen-based immunoassay-enzyme-linked immunoelectrodiffusion assay (ELIEDA)-was standardized for screening for this bacterial infection. Combining counter immunoelectrophoresis with an immunoenzymatic assay, the ELIEDA requires concentrated urine specimens, a cellulose acetate membrane, polyclonal antibodies to E. coli raised in rabbits, and peroxidase-labeled sheep antibodies to rabbit immunoglobulin G (IgG). This ELIEDA technique was evaluated using 244 urine specimens, 76 of them with E. coli, 47 with heterologous bacteria, and 121 without bacteria. In comparison to bacteriologic methods, the sensitivity, specificity, and positive and negative predictive values for the ELIEDA were 93.4%, 98.2%, 95.9%, and 97.1%, respectively. The data obtained suggest that this assay is useful for routine diagnostic screening for UTI caused by E. coli. In addition, since the ELIEDA stained membranes can be stored, this assay makes retrospective studies possible. |
format |
Article in Journal/Newspaper |
author |
Myriam T. N. Campanhã Sumie Hoshino-Shimizu Marina Baquerizo Martinez |
author_facet |
Myriam T. N. Campanhã Sumie Hoshino-Shimizu Marina Baquerizo Martinez |
author_sort |
Myriam T. N. Campanhã |
title |
Urinary tract infection: detection of Escherichia coli antigens in human urine with an ELIEDA immunoenzymatic assay |
title_short |
Urinary tract infection: detection of Escherichia coli antigens in human urine with an ELIEDA immunoenzymatic assay |
title_full |
Urinary tract infection: detection of Escherichia coli antigens in human urine with an ELIEDA immunoenzymatic assay |
title_fullStr |
Urinary tract infection: detection of Escherichia coli antigens in human urine with an ELIEDA immunoenzymatic assay |
title_full_unstemmed |
Urinary tract infection: detection of Escherichia coli antigens in human urine with an ELIEDA immunoenzymatic assay |
title_sort |
urinary tract infection: detection of escherichia coli antigens in human urine with an elieda immunoenzymatic assay |
publisher |
Pan American Health Organization |
publishDate |
1999 |
url |
https://doi.org/10.1590/s1020-49891999000700002 https://doaj.org/article/d1fb94362d1b49bf8ea864839e91a3fa |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista Panamericana de Salud Pública, Vol 6, Iss 2, Pp 89-94 (1999) |
op_relation |
http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49891999000700002&lng=en&tlng=en https://doaj.org/toc/1020-4989 1020-4989 doi:10.1590/s1020-49891999000700002 https://doaj.org/article/d1fb94362d1b49bf8ea864839e91a3fa |
op_doi |
https://doi.org/10.1590/s1020-49891999000700002 |
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Revista Panamericana de Salud Pública |
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6 |
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2 |
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94 |
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