Testing for Mycobacterium tuberculosis infection using the QuantiFERON-TB GOLD assay in patients with comorbid conditions in a tertiary care endemic setting
Abstract Introduction There were 10 million new cases of tuberculosis (TB) in 2017. To eliminate TB, it is necessary to diagnose active TB and latent tuberculosis infection (LTBI). Diagnosis of paucibacillary disease and in extrapulmonary TB (EPTB) remains challenging; low mycobacterial load can be...
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ftdoajarticles:oai:doaj.org/article:d1d9631b172e4627aefa75e1bdcc131e 2023-05-15T15:13:19+02:00 Testing for Mycobacterium tuberculosis infection using the QuantiFERON-TB GOLD assay in patients with comorbid conditions in a tertiary care endemic setting Kiran Iqbal Masood Bushra Jamil Alnoor Akber Maheen Hassan Muniba Islam Zahra Hasan 2020-02-01T00:00:00Z https://doi.org/10.1186/s40794-020-0102-z https://doaj.org/article/d1d9631b172e4627aefa75e1bdcc131e EN eng BMC http://link.springer.com/article/10.1186/s40794-020-0102-z https://doaj.org/toc/2055-0936 doi:10.1186/s40794-020-0102-z 2055-0936 https://doaj.org/article/d1d9631b172e4627aefa75e1bdcc131e Tropical Diseases, Travel Medicine and Vaccines, Vol 6, Iss 1, Pp 1-8 (2020) Tuberculosis QuantiFERON-TB GOLD Co-morbids Interferon gamma release assay Pulmonary TB Extra-pulmonary TB Arctic medicine. Tropical medicine RC955-962 article 2020 ftdoajarticles https://doi.org/10.1186/s40794-020-0102-z 2022-12-31T03:47:06Z Abstract Introduction There were 10 million new cases of tuberculosis (TB) in 2017. To eliminate TB, it is necessary to diagnose active TB and latent tuberculosis infection (LTBI). Diagnosis of paucibacillary disease and in extrapulmonary TB (EPTB) remains challenging; low mycobacterial load can be missed by microbiological or molecular based confirmation; EPTB, can be misdiagnosed due to absence of site specific specimens for testing. Interferon gamma release assays (IGRA) use T cell-based Interferon-gamma (IFN-γ) to identify infection with M. tuberculosis (MTB) but cannot discriminate between active and LTBI. We investigated how IGRA was being used in a high burden low resource setting. Methods We conducted a retrospective review of 149 consecutive cases received for QuantiFERON-TB Gold In-Tube Assay (QFT-GIT) testing in routine clinical service. Results Fifty-six cases were QFT-GIT positive and 93 were QFT-GIT negative. Thirty-six per cent of QFT-GIT tested cases had active TB. Of QFT-GIT positive cases, 59% patients had active TB; 10 with pulmonary and 23 with extra-pulmonary TB. The remaining 41% QFT-positive cases were LTBI. Of the QFT-GIT negative cases, 22% had active TB. Co-morbid conditions were present in 37% of QFT-GIT positive and 60% of QFT-GIT negative cases. Conclusions Our study shows that IGRA is being used as an adjunct test for active TB in this population. It highlights the complexity of interpreting QFT-GIT results particularly for QFT-GIT negative cases when ruling out MTB infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Tropical Diseases, Travel Medicine and Vaccines 6 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
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Tuberculosis QuantiFERON-TB GOLD Co-morbids Interferon gamma release assay Pulmonary TB Extra-pulmonary TB Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Tuberculosis QuantiFERON-TB GOLD Co-morbids Interferon gamma release assay Pulmonary TB Extra-pulmonary TB Arctic medicine. Tropical medicine RC955-962 Kiran Iqbal Masood Bushra Jamil Alnoor Akber Maheen Hassan Muniba Islam Zahra Hasan Testing for Mycobacterium tuberculosis infection using the QuantiFERON-TB GOLD assay in patients with comorbid conditions in a tertiary care endemic setting |
topic_facet |
Tuberculosis QuantiFERON-TB GOLD Co-morbids Interferon gamma release assay Pulmonary TB Extra-pulmonary TB Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract Introduction There were 10 million new cases of tuberculosis (TB) in 2017. To eliminate TB, it is necessary to diagnose active TB and latent tuberculosis infection (LTBI). Diagnosis of paucibacillary disease and in extrapulmonary TB (EPTB) remains challenging; low mycobacterial load can be missed by microbiological or molecular based confirmation; EPTB, can be misdiagnosed due to absence of site specific specimens for testing. Interferon gamma release assays (IGRA) use T cell-based Interferon-gamma (IFN-γ) to identify infection with M. tuberculosis (MTB) but cannot discriminate between active and LTBI. We investigated how IGRA was being used in a high burden low resource setting. Methods We conducted a retrospective review of 149 consecutive cases received for QuantiFERON-TB Gold In-Tube Assay (QFT-GIT) testing in routine clinical service. Results Fifty-six cases were QFT-GIT positive and 93 were QFT-GIT negative. Thirty-six per cent of QFT-GIT tested cases had active TB. Of QFT-GIT positive cases, 59% patients had active TB; 10 with pulmonary and 23 with extra-pulmonary TB. The remaining 41% QFT-positive cases were LTBI. Of the QFT-GIT negative cases, 22% had active TB. Co-morbid conditions were present in 37% of QFT-GIT positive and 60% of QFT-GIT negative cases. Conclusions Our study shows that IGRA is being used as an adjunct test for active TB in this population. It highlights the complexity of interpreting QFT-GIT results particularly for QFT-GIT negative cases when ruling out MTB infection. |
format |
Article in Journal/Newspaper |
author |
Kiran Iqbal Masood Bushra Jamil Alnoor Akber Maheen Hassan Muniba Islam Zahra Hasan |
author_facet |
Kiran Iqbal Masood Bushra Jamil Alnoor Akber Maheen Hassan Muniba Islam Zahra Hasan |
author_sort |
Kiran Iqbal Masood |
title |
Testing for Mycobacterium tuberculosis infection using the QuantiFERON-TB GOLD assay in patients with comorbid conditions in a tertiary care endemic setting |
title_short |
Testing for Mycobacterium tuberculosis infection using the QuantiFERON-TB GOLD assay in patients with comorbid conditions in a tertiary care endemic setting |
title_full |
Testing for Mycobacterium tuberculosis infection using the QuantiFERON-TB GOLD assay in patients with comorbid conditions in a tertiary care endemic setting |
title_fullStr |
Testing for Mycobacterium tuberculosis infection using the QuantiFERON-TB GOLD assay in patients with comorbid conditions in a tertiary care endemic setting |
title_full_unstemmed |
Testing for Mycobacterium tuberculosis infection using the QuantiFERON-TB GOLD assay in patients with comorbid conditions in a tertiary care endemic setting |
title_sort |
testing for mycobacterium tuberculosis infection using the quantiferon-tb gold assay in patients with comorbid conditions in a tertiary care endemic setting |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s40794-020-0102-z https://doaj.org/article/d1d9631b172e4627aefa75e1bdcc131e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Tropical Diseases, Travel Medicine and Vaccines, Vol 6, Iss 1, Pp 1-8 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s40794-020-0102-z https://doaj.org/toc/2055-0936 doi:10.1186/s40794-020-0102-z 2055-0936 https://doaj.org/article/d1d9631b172e4627aefa75e1bdcc131e |
op_doi |
https://doi.org/10.1186/s40794-020-0102-z |
container_title |
Tropical Diseases, Travel Medicine and Vaccines |
container_volume |
6 |
container_issue |
1 |
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1766343890717114368 |