A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas
Light-sensitivity is important for mollusc survival, as it plays a vital role in reproduction and predator avoidance. Light-sensitivity has been demonstrated in the adult Pacific oyster Crassostrea gigas, but the genes associated with light-sensitivity remain unclear. In the present study, we design...
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ftdoajarticles:oai:doaj.org/article:d1be5bf2f5914b51a8ef5c5315d59751 2023-05-15T15:57:57+02:00 A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas Changlu Wu Qiuyun Jiang Lei Wei Zhongqiang Cai Jun Chen Wenchao Yu Cheng He Jiao Wang Wen Guo Xiaotong Wang 2018-03-01T00:00:00Z https://doi.org/10.3389/fphys.2018.00221 https://doaj.org/article/d1be5bf2f5914b51a8ef5c5315d59751 EN eng Frontiers Media S.A. http://journal.frontiersin.org/article/10.3389/fphys.2018.00221/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2018.00221 https://doaj.org/article/d1be5bf2f5914b51a8ef5c5315d59751 Frontiers in Physiology, Vol 9 (2018) adult oyster light-sensitivity RNAi mantle rhodopsin-like superfamily member gene Physiology QP1-981 article 2018 ftdoajarticles https://doi.org/10.3389/fphys.2018.00221 2022-12-31T14:11:46Z Light-sensitivity is important for mollusc survival, as it plays a vital role in reproduction and predator avoidance. Light-sensitivity has been demonstrated in the adult Pacific oyster Crassostrea gigas, but the genes associated with light-sensitivity remain unclear. In the present study, we designed experiments to identify the genes associated with light-sensitivity in adult oysters. First, we assessed the Pacific oyster genome and identified 368 genes annotated with the terms associated with light-sensitivity. Second, the function of the four rhodopsin-like superfamily member genes was tested by using RNAi. The results showed that the highest level of mRNA expression of the vision-related genes was in the mantle; however, this finding is not true for all oyster genes. Interestingly, we also found four rhodopsin-like superfamily member genes expressed at an very high level in the mantle tissue. In the RNAi experiment, when one of rhodopsin-like superfamily member genes (CGI_1001253) was inhibited, the light-sensitivity capacity of the injected oysters was significantly reduced, suggesting that CGI_10012534 may be associated with light-sensitivity in the adult Pacific oyster. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Frontiers in Physiology 9 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
adult oyster light-sensitivity RNAi mantle rhodopsin-like superfamily member gene Physiology QP1-981 |
spellingShingle |
adult oyster light-sensitivity RNAi mantle rhodopsin-like superfamily member gene Physiology QP1-981 Changlu Wu Qiuyun Jiang Lei Wei Zhongqiang Cai Jun Chen Wenchao Yu Cheng He Jiao Wang Wen Guo Xiaotong Wang A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas |
topic_facet |
adult oyster light-sensitivity RNAi mantle rhodopsin-like superfamily member gene Physiology QP1-981 |
description |
Light-sensitivity is important for mollusc survival, as it plays a vital role in reproduction and predator avoidance. Light-sensitivity has been demonstrated in the adult Pacific oyster Crassostrea gigas, but the genes associated with light-sensitivity remain unclear. In the present study, we designed experiments to identify the genes associated with light-sensitivity in adult oysters. First, we assessed the Pacific oyster genome and identified 368 genes annotated with the terms associated with light-sensitivity. Second, the function of the four rhodopsin-like superfamily member genes was tested by using RNAi. The results showed that the highest level of mRNA expression of the vision-related genes was in the mantle; however, this finding is not true for all oyster genes. Interestingly, we also found four rhodopsin-like superfamily member genes expressed at an very high level in the mantle tissue. In the RNAi experiment, when one of rhodopsin-like superfamily member genes (CGI_1001253) was inhibited, the light-sensitivity capacity of the injected oysters was significantly reduced, suggesting that CGI_10012534 may be associated with light-sensitivity in the adult Pacific oyster. |
format |
Article in Journal/Newspaper |
author |
Changlu Wu Qiuyun Jiang Lei Wei Zhongqiang Cai Jun Chen Wenchao Yu Cheng He Jiao Wang Wen Guo Xiaotong Wang |
author_facet |
Changlu Wu Qiuyun Jiang Lei Wei Zhongqiang Cai Jun Chen Wenchao Yu Cheng He Jiao Wang Wen Guo Xiaotong Wang |
author_sort |
Changlu Wu |
title |
A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas |
title_short |
A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas |
title_full |
A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas |
title_fullStr |
A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas |
title_full_unstemmed |
A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas |
title_sort |
rhodopsin-like gene may be associated with the light-sensitivity of adult pacific oyster crassostrea gigas |
publisher |
Frontiers Media S.A. |
publishDate |
2018 |
url |
https://doi.org/10.3389/fphys.2018.00221 https://doaj.org/article/d1be5bf2f5914b51a8ef5c5315d59751 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Frontiers in Physiology, Vol 9 (2018) |
op_relation |
http://journal.frontiersin.org/article/10.3389/fphys.2018.00221/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2018.00221 https://doaj.org/article/d1be5bf2f5914b51a8ef5c5315d59751 |
op_doi |
https://doi.org/10.3389/fphys.2018.00221 |
container_title |
Frontiers in Physiology |
container_volume |
9 |
_version_ |
1766393673109471232 |