Infectious salmon anaemia virus (ISAV) isolated from the ISA disease outbreaks in Chile diverged from ISAV isolates from Norway around 1996 and was disseminated around 2005, based on surface glycoprotein gene sequences

Abstract Background Infectious salmon anaemia (ISA) virus (ISAV) is a pathogen of marine-farmed Atlantic salmon ( Salmo salar ); a disease first diagnosed in Norway in 1984. For over 25 years ISAV has caused major disease outbreaks in the Northern hemisphere, and remains an emerging fish pathogen be...

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Published in:Virology Journal
Main Authors: Lisperger Angelica, Mansilla Soledad, Gherardelli Valentina, Kibenge Molly JT, Godoy Marcos G, Wang Yingwei, Kibenge Frederick SB, Jarpa Miguel, Larroquete Geraldine, Avendaño Fernando, Lara Marcela, Gallardo Alicia
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2009
Subjects:
Online Access:https://doi.org/10.1186/1743-422X-6-88
https://doaj.org/article/d17bf5db2d674ef3b3e0023257b89349
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spelling ftdoajarticles:oai:doaj.org/article:d17bf5db2d674ef3b3e0023257b89349 2023-05-15T15:32:10+02:00 Infectious salmon anaemia virus (ISAV) isolated from the ISA disease outbreaks in Chile diverged from ISAV isolates from Norway around 1996 and was disseminated around 2005, based on surface glycoprotein gene sequences Lisperger Angelica Mansilla Soledad Gherardelli Valentina Kibenge Molly JT Godoy Marcos G Wang Yingwei Kibenge Frederick SB Jarpa Miguel Larroquete Geraldine Avendaño Fernando Lara Marcela Gallardo Alicia 2009-06-01T00:00:00Z https://doi.org/10.1186/1743-422X-6-88 https://doaj.org/article/d17bf5db2d674ef3b3e0023257b89349 EN eng BMC http://www.virologyj.com/content/6/1/88 https://doaj.org/toc/1743-422X doi:10.1186/1743-422X-6-88 1743-422X https://doaj.org/article/d17bf5db2d674ef3b3e0023257b89349 Virology Journal, Vol 6, Iss 1, p 88 (2009) Infectious and parasitic diseases RC109-216 article 2009 ftdoajarticles https://doi.org/10.1186/1743-422X-6-88 2022-12-31T04:31:26Z Abstract Background Infectious salmon anaemia (ISA) virus (ISAV) is a pathogen of marine-farmed Atlantic salmon ( Salmo salar ); a disease first diagnosed in Norway in 1984. For over 25 years ISAV has caused major disease outbreaks in the Northern hemisphere, and remains an emerging fish pathogen because of the asymptomatic infections in marine wild fish and the potential for emergence of new epidemic strains. ISAV belongs to the family Orthomyxoviridae , together with influenza viruses but is sufficiently different to be assigned to its own genus, Isavirus . The Isavirus genome consists of eight single-stranded RNA species, and the virions have two surface glycoproteins; fusion (F) protein encoded on segment 5 and haemagglutinin-esterase (HE) protein encoded on segment 6. However, comparision between different ISAV isolates is complicated because there is presently no universally accepted nomenclature system for designation of genetic relatedness between ISAV isolates. The first outbreak of ISA in marine-farmed Atlantic salmon in the Southern hemisphere occurred in Chile starting in June 2007. In order to describe the molecular characteristics of the virus so as to understand its origins, how ISAV isolates are maintained and spread, and their virulence characteristics, we conducted a study where the viral sequences were directly amplified, cloned and sequenced from tissue samples collected from several ISA-affected fish on the different fish farms with confirmed or suspected ISA outbreaks in Chile. This paper describes the genetic characterization of a large number of ISAV strains associated with extensive outbreaks in Chile starting in June 2007, and their phylogenetic relationships with selected European and North American isolates that are representative of the genetic diversity of ISAV. Results RT-PCR for ISAV F and HE glycoprotein genes was performed directly on tissue samples collected from ISA-affected fish on different farms among 14 fish companies in Chile during the ISA outbreaks that started in June ... Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Norway Virology Journal 6 1 88
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Infectious and parasitic diseases
RC109-216
spellingShingle Infectious and parasitic diseases
RC109-216
Lisperger Angelica
Mansilla Soledad
Gherardelli Valentina
Kibenge Molly JT
Godoy Marcos G
Wang Yingwei
Kibenge Frederick SB
Jarpa Miguel
Larroquete Geraldine
Avendaño Fernando
Lara Marcela
Gallardo Alicia
Infectious salmon anaemia virus (ISAV) isolated from the ISA disease outbreaks in Chile diverged from ISAV isolates from Norway around 1996 and was disseminated around 2005, based on surface glycoprotein gene sequences
topic_facet Infectious and parasitic diseases
RC109-216
description Abstract Background Infectious salmon anaemia (ISA) virus (ISAV) is a pathogen of marine-farmed Atlantic salmon ( Salmo salar ); a disease first diagnosed in Norway in 1984. For over 25 years ISAV has caused major disease outbreaks in the Northern hemisphere, and remains an emerging fish pathogen because of the asymptomatic infections in marine wild fish and the potential for emergence of new epidemic strains. ISAV belongs to the family Orthomyxoviridae , together with influenza viruses but is sufficiently different to be assigned to its own genus, Isavirus . The Isavirus genome consists of eight single-stranded RNA species, and the virions have two surface glycoproteins; fusion (F) protein encoded on segment 5 and haemagglutinin-esterase (HE) protein encoded on segment 6. However, comparision between different ISAV isolates is complicated because there is presently no universally accepted nomenclature system for designation of genetic relatedness between ISAV isolates. The first outbreak of ISA in marine-farmed Atlantic salmon in the Southern hemisphere occurred in Chile starting in June 2007. In order to describe the molecular characteristics of the virus so as to understand its origins, how ISAV isolates are maintained and spread, and their virulence characteristics, we conducted a study where the viral sequences were directly amplified, cloned and sequenced from tissue samples collected from several ISA-affected fish on the different fish farms with confirmed or suspected ISA outbreaks in Chile. This paper describes the genetic characterization of a large number of ISAV strains associated with extensive outbreaks in Chile starting in June 2007, and their phylogenetic relationships with selected European and North American isolates that are representative of the genetic diversity of ISAV. Results RT-PCR for ISAV F and HE glycoprotein genes was performed directly on tissue samples collected from ISA-affected fish on different farms among 14 fish companies in Chile during the ISA outbreaks that started in June ...
format Article in Journal/Newspaper
author Lisperger Angelica
Mansilla Soledad
Gherardelli Valentina
Kibenge Molly JT
Godoy Marcos G
Wang Yingwei
Kibenge Frederick SB
Jarpa Miguel
Larroquete Geraldine
Avendaño Fernando
Lara Marcela
Gallardo Alicia
author_facet Lisperger Angelica
Mansilla Soledad
Gherardelli Valentina
Kibenge Molly JT
Godoy Marcos G
Wang Yingwei
Kibenge Frederick SB
Jarpa Miguel
Larroquete Geraldine
Avendaño Fernando
Lara Marcela
Gallardo Alicia
author_sort Lisperger Angelica
title Infectious salmon anaemia virus (ISAV) isolated from the ISA disease outbreaks in Chile diverged from ISAV isolates from Norway around 1996 and was disseminated around 2005, based on surface glycoprotein gene sequences
title_short Infectious salmon anaemia virus (ISAV) isolated from the ISA disease outbreaks in Chile diverged from ISAV isolates from Norway around 1996 and was disseminated around 2005, based on surface glycoprotein gene sequences
title_full Infectious salmon anaemia virus (ISAV) isolated from the ISA disease outbreaks in Chile diverged from ISAV isolates from Norway around 1996 and was disseminated around 2005, based on surface glycoprotein gene sequences
title_fullStr Infectious salmon anaemia virus (ISAV) isolated from the ISA disease outbreaks in Chile diverged from ISAV isolates from Norway around 1996 and was disseminated around 2005, based on surface glycoprotein gene sequences
title_full_unstemmed Infectious salmon anaemia virus (ISAV) isolated from the ISA disease outbreaks in Chile diverged from ISAV isolates from Norway around 1996 and was disseminated around 2005, based on surface glycoprotein gene sequences
title_sort infectious salmon anaemia virus (isav) isolated from the isa disease outbreaks in chile diverged from isav isolates from norway around 1996 and was disseminated around 2005, based on surface glycoprotein gene sequences
publisher BMC
publishDate 2009
url https://doi.org/10.1186/1743-422X-6-88
https://doaj.org/article/d17bf5db2d674ef3b3e0023257b89349
geographic Norway
geographic_facet Norway
genre Atlantic salmon
Salmo salar
genre_facet Atlantic salmon
Salmo salar
op_source Virology Journal, Vol 6, Iss 1, p 88 (2009)
op_relation http://www.virologyj.com/content/6/1/88
https://doaj.org/toc/1743-422X
doi:10.1186/1743-422X-6-88
1743-422X
https://doaj.org/article/d17bf5db2d674ef3b3e0023257b89349
op_doi https://doi.org/10.1186/1743-422X-6-88
container_title Virology Journal
container_volume 6
container_issue 1
container_start_page 88
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