Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica
We compared physiological characteristics and responses to experimental freezing and thawing in winter and spring samples of the wood frog, Rana sylvatica, indigenous to Interior Alaska, USA. Whereas winter frogs can survive freezing at temperatures at least as low as −16°C, the lower limit of toler...
Published in: | International Journal of Zoology |
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ftdoajarticles:oai:doaj.org/article:d146ee5d45c6410d8f63fc6937d11dd1 2024-09-09T20:10:49+00:00 Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica Jon P. Costanzo M. Clara F. do Amaral Andrew J. Rosendale Richard E. Lee 2014-01-01T00:00:00Z https://doi.org/10.1155/2014/750153 https://doaj.org/article/d146ee5d45c6410d8f63fc6937d11dd1 EN eng Wiley http://dx.doi.org/10.1155/2014/750153 https://doaj.org/toc/1687-8477 https://doaj.org/toc/1687-8485 1687-8477 1687-8485 doi:10.1155/2014/750153 https://doaj.org/article/d146ee5d45c6410d8f63fc6937d11dd1 International Journal of Zoology, Vol 2014 (2014) Zoology QL1-991 article 2014 ftdoajarticles https://doi.org/10.1155/2014/750153 2024-08-05T17:48:42Z We compared physiological characteristics and responses to experimental freezing and thawing in winter and spring samples of the wood frog, Rana sylvatica, indigenous to Interior Alaska, USA. Whereas winter frogs can survive freezing at temperatures at least as low as −16°C, the lower limit of tolerance for spring frogs was between −2.5°C and −5°C. Spring frogs had comparatively low levels of the urea in blood plasma, liver, heart, brain, and skeletal muscle, as well as a smaller hepatic reserve of glycogen, which is converted to glucose after freezing begins. Consequently, following freezing (−2.5°C, 48 h) tissue concentrations of these cryoprotective osmolytes were 44–88% lower than those measured in winter frogs. Spring frogs formed much more ice and incurred extensive cryohemolysis and lactate accrual, indicating that they had suffered marked cell damage and hypoxic stress during freezing. Multiple, interactive stresses, in addition to diminished cryoprotectant levels, contribute to the reduced capacity for freeze tolerance in posthibernal frogs. Article in Journal/Newspaper Subarctic Alaska Directory of Open Access Journals: DOAJ Articles International Journal of Zoology 2014 1 13 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Zoology QL1-991 |
spellingShingle |
Zoology QL1-991 Jon P. Costanzo M. Clara F. do Amaral Andrew J. Rosendale Richard E. Lee Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica |
topic_facet |
Zoology QL1-991 |
description |
We compared physiological characteristics and responses to experimental freezing and thawing in winter and spring samples of the wood frog, Rana sylvatica, indigenous to Interior Alaska, USA. Whereas winter frogs can survive freezing at temperatures at least as low as −16°C, the lower limit of tolerance for spring frogs was between −2.5°C and −5°C. Spring frogs had comparatively low levels of the urea in blood plasma, liver, heart, brain, and skeletal muscle, as well as a smaller hepatic reserve of glycogen, which is converted to glucose after freezing begins. Consequently, following freezing (−2.5°C, 48 h) tissue concentrations of these cryoprotective osmolytes were 44–88% lower than those measured in winter frogs. Spring frogs formed much more ice and incurred extensive cryohemolysis and lactate accrual, indicating that they had suffered marked cell damage and hypoxic stress during freezing. Multiple, interactive stresses, in addition to diminished cryoprotectant levels, contribute to the reduced capacity for freeze tolerance in posthibernal frogs. |
format |
Article in Journal/Newspaper |
author |
Jon P. Costanzo M. Clara F. do Amaral Andrew J. Rosendale Richard E. Lee |
author_facet |
Jon P. Costanzo M. Clara F. do Amaral Andrew J. Rosendale Richard E. Lee |
author_sort |
Jon P. Costanzo |
title |
Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica |
title_short |
Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica |
title_full |
Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica |
title_fullStr |
Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica |
title_full_unstemmed |
Seasonality of Freeze Tolerance in a Subarctic Population of the Wood Frog, Rana sylvatica |
title_sort |
seasonality of freeze tolerance in a subarctic population of the wood frog, rana sylvatica |
publisher |
Wiley |
publishDate |
2014 |
url |
https://doi.org/10.1155/2014/750153 https://doaj.org/article/d146ee5d45c6410d8f63fc6937d11dd1 |
genre |
Subarctic Alaska |
genre_facet |
Subarctic Alaska |
op_source |
International Journal of Zoology, Vol 2014 (2014) |
op_relation |
http://dx.doi.org/10.1155/2014/750153 https://doaj.org/toc/1687-8477 https://doaj.org/toc/1687-8485 1687-8477 1687-8485 doi:10.1155/2014/750153 https://doaj.org/article/d146ee5d45c6410d8f63fc6937d11dd1 |
op_doi |
https://doi.org/10.1155/2014/750153 |
container_title |
International Journal of Zoology |
container_volume |
2014 |
container_start_page |
1 |
op_container_end_page |
13 |
_version_ |
1809945246848516096 |