Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle
Isoamyl acetate synthesis employing aqueous Candida antarctica lipase B (CaLB) solution was performed in a two-phase solvent system comprising hydrophilic ionic liquid 1-butyl-3-methylpyridinium dicyanamide and n-heptane. An X-junction glass microfluidic chip was used to obtain uniform microdroplets...
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De Gruyter
2013
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ftdoajarticles:oai:doaj.org/article:d135c74b60944a6fbc4062b75d96359d 2023-05-15T13:49:31+02:00 Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle Novak Uroš Žnidaršič-Plazl Polona 2013-12-01T00:00:00Z https://doi.org/10.1515/gps-2013-0082 https://doaj.org/article/d135c74b60944a6fbc4062b75d96359d EN eng De Gruyter https://doi.org/10.1515/gps-2013-0082 https://doaj.org/toc/2191-9542 https://doaj.org/toc/2191-9550 2191-9542 2191-9550 doi:10.1515/gps-2013-0082 https://doaj.org/article/d135c74b60944a6fbc4062b75d96359d Green Processing and Synthesis, Vol 2, Iss 6, Pp 561-568 (2013) esterification ionic liquid lipase b microreactor process integration Chemistry QD1-999 article 2013 ftdoajarticles https://doi.org/10.1515/gps-2013-0082 2022-12-31T11:42:27Z Isoamyl acetate synthesis employing aqueous Candida antarctica lipase B (CaLB) solution was performed in a two-phase solvent system comprising hydrophilic ionic liquid 1-butyl-3-methylpyridinium dicyanamide and n-heptane. An X-junction glass microfluidic chip was used to obtain uniform microdroplets of n-heptane within a continuous phase of ionic liquid with dissolved enzyme and reactants, namely, isoamyl alcohol and acetic anhydride. A developed flow pattern resulted in a very large specific interfacial area for the reaction with amphiphilic CaLB and simultaneous extraction of isoamyl acetate in n-heptane. Biotransformation was performed within a continuously operated microfluidic system consisting of an X-junction chip and a silanized tube of a submillimeter diameter, which was further integrated with a microseparator based on a hydrophobic membrane. Efficient separation of n-heptane with product from ionic liquid phase containing enzyme and remaining substrates and products was achieved, enabling reuse of CaLB together with ionic liquid phase. More than 80% of productivity was preserved in each of the eight consecutive biotransformations within the integrated microfluidic system with reused lipase B in the ionic liquid phase. Subsequent ionic liquid regeneration accomplished by vacuum distillation enabled efficient reuse of this solvent in esterification with fresh enzyme. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Green Processing and Synthesis 2 6 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
esterification ionic liquid lipase b microreactor process integration Chemistry QD1-999 |
spellingShingle |
esterification ionic liquid lipase b microreactor process integration Chemistry QD1-999 Novak Uroš Žnidaršič-Plazl Polona Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle |
topic_facet |
esterification ionic liquid lipase b microreactor process integration Chemistry QD1-999 |
description |
Isoamyl acetate synthesis employing aqueous Candida antarctica lipase B (CaLB) solution was performed in a two-phase solvent system comprising hydrophilic ionic liquid 1-butyl-3-methylpyridinium dicyanamide and n-heptane. An X-junction glass microfluidic chip was used to obtain uniform microdroplets of n-heptane within a continuous phase of ionic liquid with dissolved enzyme and reactants, namely, isoamyl alcohol and acetic anhydride. A developed flow pattern resulted in a very large specific interfacial area for the reaction with amphiphilic CaLB and simultaneous extraction of isoamyl acetate in n-heptane. Biotransformation was performed within a continuously operated microfluidic system consisting of an X-junction chip and a silanized tube of a submillimeter diameter, which was further integrated with a microseparator based on a hydrophobic membrane. Efficient separation of n-heptane with product from ionic liquid phase containing enzyme and remaining substrates and products was achieved, enabling reuse of CaLB together with ionic liquid phase. More than 80% of productivity was preserved in each of the eight consecutive biotransformations within the integrated microfluidic system with reused lipase B in the ionic liquid phase. Subsequent ionic liquid regeneration accomplished by vacuum distillation enabled efficient reuse of this solvent in esterification with fresh enzyme. |
format |
Article in Journal/Newspaper |
author |
Novak Uroš Žnidaršič-Plazl Polona |
author_facet |
Novak Uroš Žnidaršič-Plazl Polona |
author_sort |
Novak Uroš |
title |
Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle |
title_short |
Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle |
title_full |
Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle |
title_fullStr |
Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle |
title_full_unstemmed |
Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle |
title_sort |
integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle |
publisher |
De Gruyter |
publishDate |
2013 |
url |
https://doi.org/10.1515/gps-2013-0082 https://doaj.org/article/d135c74b60944a6fbc4062b75d96359d |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Green Processing and Synthesis, Vol 2, Iss 6, Pp 561-568 (2013) |
op_relation |
https://doi.org/10.1515/gps-2013-0082 https://doaj.org/toc/2191-9542 https://doaj.org/toc/2191-9550 2191-9542 2191-9550 doi:10.1515/gps-2013-0082 https://doaj.org/article/d135c74b60944a6fbc4062b75d96359d |
op_doi |
https://doi.org/10.1515/gps-2013-0082 |
container_title |
Green Processing and Synthesis |
container_volume |
2 |
container_issue |
6 |
_version_ |
1766251465435774976 |