Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle

Isoamyl acetate synthesis employing aqueous Candida antarctica lipase B (CaLB) solution was performed in a two-phase solvent system comprising hydrophilic ionic liquid 1-butyl-3-methylpyridinium dicyanamide and n-heptane. An X-junction glass microfluidic chip was used to obtain uniform microdroplets...

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Published in:Green Processing and Synthesis
Main Authors: Novak Uroš, Žnidaršič-Plazl Polona
Format: Article in Journal/Newspaper
Language:English
Published: De Gruyter 2013
Subjects:
Online Access:https://doi.org/10.1515/gps-2013-0082
https://doaj.org/article/d135c74b60944a6fbc4062b75d96359d
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spelling ftdoajarticles:oai:doaj.org/article:d135c74b60944a6fbc4062b75d96359d 2023-05-15T13:49:31+02:00 Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle Novak Uroš Žnidaršič-Plazl Polona 2013-12-01T00:00:00Z https://doi.org/10.1515/gps-2013-0082 https://doaj.org/article/d135c74b60944a6fbc4062b75d96359d EN eng De Gruyter https://doi.org/10.1515/gps-2013-0082 https://doaj.org/toc/2191-9542 https://doaj.org/toc/2191-9550 2191-9542 2191-9550 doi:10.1515/gps-2013-0082 https://doaj.org/article/d135c74b60944a6fbc4062b75d96359d Green Processing and Synthesis, Vol 2, Iss 6, Pp 561-568 (2013) esterification ionic liquid lipase b microreactor process integration Chemistry QD1-999 article 2013 ftdoajarticles https://doi.org/10.1515/gps-2013-0082 2022-12-31T11:42:27Z Isoamyl acetate synthesis employing aqueous Candida antarctica lipase B (CaLB) solution was performed in a two-phase solvent system comprising hydrophilic ionic liquid 1-butyl-3-methylpyridinium dicyanamide and n-heptane. An X-junction glass microfluidic chip was used to obtain uniform microdroplets of n-heptane within a continuous phase of ionic liquid with dissolved enzyme and reactants, namely, isoamyl alcohol and acetic anhydride. A developed flow pattern resulted in a very large specific interfacial area for the reaction with amphiphilic CaLB and simultaneous extraction of isoamyl acetate in n-heptane. Biotransformation was performed within a continuously operated microfluidic system consisting of an X-junction chip and a silanized tube of a submillimeter diameter, which was further integrated with a microseparator based on a hydrophobic membrane. Efficient separation of n-heptane with product from ionic liquid phase containing enzyme and remaining substrates and products was achieved, enabling reuse of CaLB together with ionic liquid phase. More than 80% of productivity was preserved in each of the eight consecutive biotransformations within the integrated microfluidic system with reused lipase B in the ionic liquid phase. Subsequent ionic liquid regeneration accomplished by vacuum distillation enabled efficient reuse of this solvent in esterification with fresh enzyme. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Green Processing and Synthesis 2 6
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic esterification
ionic liquid
lipase b
microreactor
process integration
Chemistry
QD1-999
spellingShingle esterification
ionic liquid
lipase b
microreactor
process integration
Chemistry
QD1-999
Novak Uroš
Žnidaršič-Plazl Polona
Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle
topic_facet esterification
ionic liquid
lipase b
microreactor
process integration
Chemistry
QD1-999
description Isoamyl acetate synthesis employing aqueous Candida antarctica lipase B (CaLB) solution was performed in a two-phase solvent system comprising hydrophilic ionic liquid 1-butyl-3-methylpyridinium dicyanamide and n-heptane. An X-junction glass microfluidic chip was used to obtain uniform microdroplets of n-heptane within a continuous phase of ionic liquid with dissolved enzyme and reactants, namely, isoamyl alcohol and acetic anhydride. A developed flow pattern resulted in a very large specific interfacial area for the reaction with amphiphilic CaLB and simultaneous extraction of isoamyl acetate in n-heptane. Biotransformation was performed within a continuously operated microfluidic system consisting of an X-junction chip and a silanized tube of a submillimeter diameter, which was further integrated with a microseparator based on a hydrophobic membrane. Efficient separation of n-heptane with product from ionic liquid phase containing enzyme and remaining substrates and products was achieved, enabling reuse of CaLB together with ionic liquid phase. More than 80% of productivity was preserved in each of the eight consecutive biotransformations within the integrated microfluidic system with reused lipase B in the ionic liquid phase. Subsequent ionic liquid regeneration accomplished by vacuum distillation enabled efficient reuse of this solvent in esterification with fresh enzyme.
format Article in Journal/Newspaper
author Novak Uroš
Žnidaršič-Plazl Polona
author_facet Novak Uroš
Žnidaršič-Plazl Polona
author_sort Novak Uroš
title Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle
title_short Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle
title_full Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle
title_fullStr Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle
title_full_unstemmed Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle
title_sort integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle
publisher De Gruyter
publishDate 2013
url https://doi.org/10.1515/gps-2013-0082
https://doaj.org/article/d135c74b60944a6fbc4062b75d96359d
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_source Green Processing and Synthesis, Vol 2, Iss 6, Pp 561-568 (2013)
op_relation https://doi.org/10.1515/gps-2013-0082
https://doaj.org/toc/2191-9542
https://doaj.org/toc/2191-9550
2191-9542
2191-9550
doi:10.1515/gps-2013-0082
https://doaj.org/article/d135c74b60944a6fbc4062b75d96359d
op_doi https://doi.org/10.1515/gps-2013-0082
container_title Green Processing and Synthesis
container_volume 2
container_issue 6
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