Determination of in vivo toxicity and in vitro cytotoxicity of venom from the Cypriot blunt-nosed viper Macrovipera lebetina lebetina and antivenom production
The venomous Levantine viper, Macrovipera lebetina lebetina is endemic to Cyprus. The objective of this study was to investigate in vitro cytotoxicity, in vivo lethality, and antivenom production followed by a re-immunization schedule in mice against Macrovipera lebetina lebetina venom. The LD50 val...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:d122673938054ca4a66822699ff50f3d 2023-05-15T15:08:19+02:00 Determination of in vivo toxicity and in vitro cytotoxicity of venom from the Cypriot blunt-nosed viper Macrovipera lebetina lebetina and antivenom production A Nalbantsoy NU Karabay-Yavasoglu F Sayim I Deliloglu-Gurhan B Gocmen H Arikan MZ Yildiz 2012-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992012000200011 https://doaj.org/article/d122673938054ca4a66822699ff50f3d EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200011 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992012000200011 1678-9199 https://doaj.org/article/d122673938054ca4a66822699ff50f3d Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 18, Iss 2, Pp 208-216 (2012) Macrovipera lebetina lebetina LD50 lethality snake venom antivenom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2012 ftdoajarticles https://doi.org/10.1590/S1678-91992012000200011 2022-12-31T09:30:06Z The venomous Levantine viper, Macrovipera lebetina lebetina is endemic to Cyprus. The objective of this study was to investigate in vitro cytotoxicity, in vivo lethality, and antivenom production followed by a re-immunization schedule in mice against Macrovipera lebetina lebetina venom. The LD50 value was estimated as 7.58 mg/kg within 24 hours by different venom doses administrated intraperitoneally in mice. Freund's complete and incomplete adjuvants were used for first and second immunization of mice in antivenom production. A cell-based assay was performed to determine the effects of Macrovipera lebetina lebetina venom and antivenom neutralizing potency on L929 cell viability. The snake venom toxicity and cytotoxicity were examined and comparison of results showed good correlation, the LD50 value was tenfold higher than the IC50 value. The IC50 value was 0.62 ± 0.18 mL after 48 hours treatment while the calculated value was 1.62 ± 0.25 mL for the culture media totally refreshed after two hours treatment with venom. The in vitro efficacy of antivenom against Macrovipera lebetina lebetina venom was found to be low. This is the first report that describes the in vivo and in vitro toxic effects of Macrovipera lebetina lebetina venom and antivenom production against this species. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 18 2 208 216 |
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English |
topic |
Macrovipera lebetina lebetina LD50 lethality snake venom antivenom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Macrovipera lebetina lebetina LD50 lethality snake venom antivenom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 A Nalbantsoy NU Karabay-Yavasoglu F Sayim I Deliloglu-Gurhan B Gocmen H Arikan MZ Yildiz Determination of in vivo toxicity and in vitro cytotoxicity of venom from the Cypriot blunt-nosed viper Macrovipera lebetina lebetina and antivenom production |
topic_facet |
Macrovipera lebetina lebetina LD50 lethality snake venom antivenom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
The venomous Levantine viper, Macrovipera lebetina lebetina is endemic to Cyprus. The objective of this study was to investigate in vitro cytotoxicity, in vivo lethality, and antivenom production followed by a re-immunization schedule in mice against Macrovipera lebetina lebetina venom. The LD50 value was estimated as 7.58 mg/kg within 24 hours by different venom doses administrated intraperitoneally in mice. Freund's complete and incomplete adjuvants were used for first and second immunization of mice in antivenom production. A cell-based assay was performed to determine the effects of Macrovipera lebetina lebetina venom and antivenom neutralizing potency on L929 cell viability. The snake venom toxicity and cytotoxicity were examined and comparison of results showed good correlation, the LD50 value was tenfold higher than the IC50 value. The IC50 value was 0.62 ± 0.18 mL after 48 hours treatment while the calculated value was 1.62 ± 0.25 mL for the culture media totally refreshed after two hours treatment with venom. The in vitro efficacy of antivenom against Macrovipera lebetina lebetina venom was found to be low. This is the first report that describes the in vivo and in vitro toxic effects of Macrovipera lebetina lebetina venom and antivenom production against this species. |
format |
Article in Journal/Newspaper |
author |
A Nalbantsoy NU Karabay-Yavasoglu F Sayim I Deliloglu-Gurhan B Gocmen H Arikan MZ Yildiz |
author_facet |
A Nalbantsoy NU Karabay-Yavasoglu F Sayim I Deliloglu-Gurhan B Gocmen H Arikan MZ Yildiz |
author_sort |
A Nalbantsoy |
title |
Determination of in vivo toxicity and in vitro cytotoxicity of venom from the Cypriot blunt-nosed viper Macrovipera lebetina lebetina and antivenom production |
title_short |
Determination of in vivo toxicity and in vitro cytotoxicity of venom from the Cypriot blunt-nosed viper Macrovipera lebetina lebetina and antivenom production |
title_full |
Determination of in vivo toxicity and in vitro cytotoxicity of venom from the Cypriot blunt-nosed viper Macrovipera lebetina lebetina and antivenom production |
title_fullStr |
Determination of in vivo toxicity and in vitro cytotoxicity of venom from the Cypriot blunt-nosed viper Macrovipera lebetina lebetina and antivenom production |
title_full_unstemmed |
Determination of in vivo toxicity and in vitro cytotoxicity of venom from the Cypriot blunt-nosed viper Macrovipera lebetina lebetina and antivenom production |
title_sort |
determination of in vivo toxicity and in vitro cytotoxicity of venom from the cypriot blunt-nosed viper macrovipera lebetina lebetina and antivenom production |
publisher |
SciELO |
publishDate |
2012 |
url |
https://doi.org/10.1590/S1678-91992012000200011 https://doaj.org/article/d122673938054ca4a66822699ff50f3d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 18, Iss 2, Pp 208-216 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200011 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992012000200011 1678-9199 https://doaj.org/article/d122673938054ca4a66822699ff50f3d |
op_doi |
https://doi.org/10.1590/S1678-91992012000200011 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
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18 |
container_issue |
2 |
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208 |
op_container_end_page |
216 |
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1766339705535725568 |