Disease severity enhancement by an esterase from non-phytopathogenic yeast Pseudozyma antarctica and its potential as adjuvant for biocontrol agents
Abstract The phylloplane yeast Pseudozyma antarctica secretes an esterase, named PaE, and xylanase when cultivated with xylose. We previously observed that the lipophilic layer of Micro-Tom tomato leaves became thinner after the culture filtrate treatment. The leaves developed reduced water-holding...
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ftdoajarticles:oai:doaj.org/article:d0fc195f9aed4464a4ba289649a3e6a6 2023-05-15T13:41:36+02:00 Disease severity enhancement by an esterase from non-phytopathogenic yeast Pseudozyma antarctica and its potential as adjuvant for biocontrol agents Hirokazu Ueda Daisuke Kurose Soichi Kugimiya Ichiro Mitsuhara Shigenobu Yoshida Jun Tabata Ken Suzuki Hiroko Kitamoto 2018-11-01T00:00:00Z https://doi.org/10.1038/s41598-018-34705-z https://doaj.org/article/d0fc195f9aed4464a4ba289649a3e6a6 EN eng Nature Portfolio https://doi.org/10.1038/s41598-018-34705-z https://doaj.org/toc/2045-2322 doi:10.1038/s41598-018-34705-z 2045-2322 https://doaj.org/article/d0fc195f9aed4464a4ba289649a3e6a6 Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) Pseudozyma Cinerea Spores Lipophilic Layer Puccinia Phylloplane Yeasts Medicine R Science Q article 2018 ftdoajarticles https://doi.org/10.1038/s41598-018-34705-z 2022-12-31T09:03:05Z Abstract The phylloplane yeast Pseudozyma antarctica secretes an esterase, named PaE, and xylanase when cultivated with xylose. We previously observed that the lipophilic layer of Micro-Tom tomato leaves became thinner after the culture filtrate treatment. The leaves developed reduced water-holding ability and became wilted. In this study, the purified enzymes were spotted on Micro-Tom leaves. PaE, but not xylanase, thinned the lipophilic layer of leaves and decreased leaf resistance to the phytopathogenic fungus Botrytis cinerea. Disease severity increased significantly in detached leaves and potted plants treated with the culture filtrate and B. cinerea spores compared with those treated with inactivated enzyme and B. cinerea alone. Spore germination ratios, numbers of penetrating fungal hyphae in the leaves, and fungal DNA contents also increased significantly on the detached leaves. Japanese knotweed (Fallopia japonica), a serious invasive alien weed in Europe and North America, also became susceptible to infection by the rust pathogen Puccinia polygoni-amphibii var. tovariae following the culture filtrate treatment. The culture filtrate treatment increased disease development in plants induced by both phytopathogenic fungi. Our results suggest that P. antarctica culture filtrate could be used as an adjuvant for sustainable biological weed control using phytopathogenic fungi. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Scientific Reports 8 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Pseudozyma Cinerea Spores Lipophilic Layer Puccinia Phylloplane Yeasts Medicine R Science Q |
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Pseudozyma Cinerea Spores Lipophilic Layer Puccinia Phylloplane Yeasts Medicine R Science Q Hirokazu Ueda Daisuke Kurose Soichi Kugimiya Ichiro Mitsuhara Shigenobu Yoshida Jun Tabata Ken Suzuki Hiroko Kitamoto Disease severity enhancement by an esterase from non-phytopathogenic yeast Pseudozyma antarctica and its potential as adjuvant for biocontrol agents |
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Pseudozyma Cinerea Spores Lipophilic Layer Puccinia Phylloplane Yeasts Medicine R Science Q |
description |
Abstract The phylloplane yeast Pseudozyma antarctica secretes an esterase, named PaE, and xylanase when cultivated with xylose. We previously observed that the lipophilic layer of Micro-Tom tomato leaves became thinner after the culture filtrate treatment. The leaves developed reduced water-holding ability and became wilted. In this study, the purified enzymes were spotted on Micro-Tom leaves. PaE, but not xylanase, thinned the lipophilic layer of leaves and decreased leaf resistance to the phytopathogenic fungus Botrytis cinerea. Disease severity increased significantly in detached leaves and potted plants treated with the culture filtrate and B. cinerea spores compared with those treated with inactivated enzyme and B. cinerea alone. Spore germination ratios, numbers of penetrating fungal hyphae in the leaves, and fungal DNA contents also increased significantly on the detached leaves. Japanese knotweed (Fallopia japonica), a serious invasive alien weed in Europe and North America, also became susceptible to infection by the rust pathogen Puccinia polygoni-amphibii var. tovariae following the culture filtrate treatment. The culture filtrate treatment increased disease development in plants induced by both phytopathogenic fungi. Our results suggest that P. antarctica culture filtrate could be used as an adjuvant for sustainable biological weed control using phytopathogenic fungi. |
format |
Article in Journal/Newspaper |
author |
Hirokazu Ueda Daisuke Kurose Soichi Kugimiya Ichiro Mitsuhara Shigenobu Yoshida Jun Tabata Ken Suzuki Hiroko Kitamoto |
author_facet |
Hirokazu Ueda Daisuke Kurose Soichi Kugimiya Ichiro Mitsuhara Shigenobu Yoshida Jun Tabata Ken Suzuki Hiroko Kitamoto |
author_sort |
Hirokazu Ueda |
title |
Disease severity enhancement by an esterase from non-phytopathogenic yeast Pseudozyma antarctica and its potential as adjuvant for biocontrol agents |
title_short |
Disease severity enhancement by an esterase from non-phytopathogenic yeast Pseudozyma antarctica and its potential as adjuvant for biocontrol agents |
title_full |
Disease severity enhancement by an esterase from non-phytopathogenic yeast Pseudozyma antarctica and its potential as adjuvant for biocontrol agents |
title_fullStr |
Disease severity enhancement by an esterase from non-phytopathogenic yeast Pseudozyma antarctica and its potential as adjuvant for biocontrol agents |
title_full_unstemmed |
Disease severity enhancement by an esterase from non-phytopathogenic yeast Pseudozyma antarctica and its potential as adjuvant for biocontrol agents |
title_sort |
disease severity enhancement by an esterase from non-phytopathogenic yeast pseudozyma antarctica and its potential as adjuvant for biocontrol agents |
publisher |
Nature Portfolio |
publishDate |
2018 |
url |
https://doi.org/10.1038/s41598-018-34705-z https://doaj.org/article/d0fc195f9aed4464a4ba289649a3e6a6 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) |
op_relation |
https://doi.org/10.1038/s41598-018-34705-z https://doaj.org/toc/2045-2322 doi:10.1038/s41598-018-34705-z 2045-2322 https://doaj.org/article/d0fc195f9aed4464a4ba289649a3e6a6 |
op_doi |
https://doi.org/10.1038/s41598-018-34705-z |
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Scientific Reports |
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8 |
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1766152924326526976 |