A survey of prevalence and phenotypic and genotypic assessment of antibiotic resistance in Staphylococcus aureus bacteria isolated from ready-to-eat food samples collected from Tehran Province, Iran
Abstract Background Resistant Staphylococcus aureus (S. aureus) bacteria are considered among the major causes of foodborne diseases. This survey aims to assess genotypic and phenotypic profiles of antibiotic resistance in S. aureus bacteria isolated from ready-to-eat food samples. Methods According...
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ftdoajarticles:oai:doaj.org/article:d0e62ca6d2b04e968760263c1f353630 2023-05-15T15:14:48+02:00 A survey of prevalence and phenotypic and genotypic assessment of antibiotic resistance in Staphylococcus aureus bacteria isolated from ready-to-eat food samples collected from Tehran Province, Iran Arash Mesbah Zohreh Mashak Zohreh Abdolmaleki 2021-10-01T00:00:00Z https://doi.org/10.1186/s41182-021-00366-4 https://doaj.org/article/d0e62ca6d2b04e968760263c1f353630 EN eng BMC https://doi.org/10.1186/s41182-021-00366-4 https://doaj.org/toc/1349-4147 doi:10.1186/s41182-021-00366-4 1349-4147 https://doaj.org/article/d0e62ca6d2b04e968760263c1f353630 Tropical Medicine and Health, Vol 49, Iss 1, Pp 1-12 (2021) Staphylococcus aureus Prevalence Phenotype of antibiotic resistance Genotype of antibiotic resistance Ready-to-eat food Arctic medicine. Tropical medicine RC955-962 article 2021 ftdoajarticles https://doi.org/10.1186/s41182-021-00366-4 2022-12-31T13:19:32Z Abstract Background Resistant Staphylococcus aureus (S. aureus) bacteria are considered among the major causes of foodborne diseases. This survey aims to assess genotypic and phenotypic profiles of antibiotic resistance in S. aureus bacteria isolated from ready-to-eat food samples. Methods According to the previously reported prevalence of S. aureus in ready-to-eat food samples, a total of 415 ready-to-eat food samples were collected from Tehran province, Iran. S. aureus bacteria were identified using culture and biochemical tests. Besides, the phenotypic antibiotic resistance profile was determined by disk diffusion. In addition, the genotypic pattern of antibiotic resistance was determined using the PCR. Results A total of 64 out of 415 (15.42%) ready-to-eat food samples were contaminated with S. aureus. Grilled mushrooms and salad olivieh harbored the highest contamination rate of (30%), while salami samples harbored the lowest contamination rate of 3.33%. In addition, S. aureus bacteria harbored the highest prevalence of resistance to penicillin (85.93%), tetracycline (85.93%), gentamicin (73.43%), erythromycin (53.12%), trimethoprim-sulfamethoxazole (51.56%), and ciprofloxacin (50%). However, all isolates were resistant to at least four antibiotic agents. Accordingly, the prevalence of tetK (70.31%), blaZ (64.06%), aacA-D (57.81%), gyrA (50%), and ermA (39.06%) was higher than that of other detected antibiotic resistance genes. Besides, AacA-D + blaZ (48.43%), tetK + blaZ (46.87%), aacA-D + tetK (39.06%), aacA-D + gyrA (20.31%), and ermA + blaZ (20.31%) were the most frequently identified combined genotypic patterns of antibiotic resistance. Conclusion Ready-to-eat food samples may be sources of resistant S. aureus, which pose a hygienic threat in case of their consumption. However, further investigations are required to identify additional epidemiological features of S. aureus in ready-to-eat foods. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Tropical Medicine and Health 49 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Staphylococcus aureus Prevalence Phenotype of antibiotic resistance Genotype of antibiotic resistance Ready-to-eat food Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Staphylococcus aureus Prevalence Phenotype of antibiotic resistance Genotype of antibiotic resistance Ready-to-eat food Arctic medicine. Tropical medicine RC955-962 Arash Mesbah Zohreh Mashak Zohreh Abdolmaleki A survey of prevalence and phenotypic and genotypic assessment of antibiotic resistance in Staphylococcus aureus bacteria isolated from ready-to-eat food samples collected from Tehran Province, Iran |
topic_facet |
Staphylococcus aureus Prevalence Phenotype of antibiotic resistance Genotype of antibiotic resistance Ready-to-eat food Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract Background Resistant Staphylococcus aureus (S. aureus) bacteria are considered among the major causes of foodborne diseases. This survey aims to assess genotypic and phenotypic profiles of antibiotic resistance in S. aureus bacteria isolated from ready-to-eat food samples. Methods According to the previously reported prevalence of S. aureus in ready-to-eat food samples, a total of 415 ready-to-eat food samples were collected from Tehran province, Iran. S. aureus bacteria were identified using culture and biochemical tests. Besides, the phenotypic antibiotic resistance profile was determined by disk diffusion. In addition, the genotypic pattern of antibiotic resistance was determined using the PCR. Results A total of 64 out of 415 (15.42%) ready-to-eat food samples were contaminated with S. aureus. Grilled mushrooms and salad olivieh harbored the highest contamination rate of (30%), while salami samples harbored the lowest contamination rate of 3.33%. In addition, S. aureus bacteria harbored the highest prevalence of resistance to penicillin (85.93%), tetracycline (85.93%), gentamicin (73.43%), erythromycin (53.12%), trimethoprim-sulfamethoxazole (51.56%), and ciprofloxacin (50%). However, all isolates were resistant to at least four antibiotic agents. Accordingly, the prevalence of tetK (70.31%), blaZ (64.06%), aacA-D (57.81%), gyrA (50%), and ermA (39.06%) was higher than that of other detected antibiotic resistance genes. Besides, AacA-D + blaZ (48.43%), tetK + blaZ (46.87%), aacA-D + tetK (39.06%), aacA-D + gyrA (20.31%), and ermA + blaZ (20.31%) were the most frequently identified combined genotypic patterns of antibiotic resistance. Conclusion Ready-to-eat food samples may be sources of resistant S. aureus, which pose a hygienic threat in case of their consumption. However, further investigations are required to identify additional epidemiological features of S. aureus in ready-to-eat foods. |
format |
Article in Journal/Newspaper |
author |
Arash Mesbah Zohreh Mashak Zohreh Abdolmaleki |
author_facet |
Arash Mesbah Zohreh Mashak Zohreh Abdolmaleki |
author_sort |
Arash Mesbah |
title |
A survey of prevalence and phenotypic and genotypic assessment of antibiotic resistance in Staphylococcus aureus bacteria isolated from ready-to-eat food samples collected from Tehran Province, Iran |
title_short |
A survey of prevalence and phenotypic and genotypic assessment of antibiotic resistance in Staphylococcus aureus bacteria isolated from ready-to-eat food samples collected from Tehran Province, Iran |
title_full |
A survey of prevalence and phenotypic and genotypic assessment of antibiotic resistance in Staphylococcus aureus bacteria isolated from ready-to-eat food samples collected from Tehran Province, Iran |
title_fullStr |
A survey of prevalence and phenotypic and genotypic assessment of antibiotic resistance in Staphylococcus aureus bacteria isolated from ready-to-eat food samples collected from Tehran Province, Iran |
title_full_unstemmed |
A survey of prevalence and phenotypic and genotypic assessment of antibiotic resistance in Staphylococcus aureus bacteria isolated from ready-to-eat food samples collected from Tehran Province, Iran |
title_sort |
survey of prevalence and phenotypic and genotypic assessment of antibiotic resistance in staphylococcus aureus bacteria isolated from ready-to-eat food samples collected from tehran province, iran |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s41182-021-00366-4 https://doaj.org/article/d0e62ca6d2b04e968760263c1f353630 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Tropical Medicine and Health, Vol 49, Iss 1, Pp 1-12 (2021) |
op_relation |
https://doi.org/10.1186/s41182-021-00366-4 https://doaj.org/toc/1349-4147 doi:10.1186/s41182-021-00366-4 1349-4147 https://doaj.org/article/d0e62ca6d2b04e968760263c1f353630 |
op_doi |
https://doi.org/10.1186/s41182-021-00366-4 |
container_title |
Tropical Medicine and Health |
container_volume |
49 |
container_issue |
1 |
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1766345217698430976 |