Diversity and Activity of Sulfate-Reducing Prokaryotes in Kamchatka Hot Springs
Microbial communities of the Kamchatka Peninsula terrestrial hot springs were studied using radioisotopic and cultural approaches, as well as by the amplification and sequencing of dsrB and 16S rRNA genes fragments. Radioisotopic experiments with 35 S-labeled sulfate showed that microbial communitie...
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ftdoajarticles:oai:doaj.org/article:d0dabe4ccf3a47648a92a9701a1304a9 2023-05-15T16:58:29+02:00 Diversity and Activity of Sulfate-Reducing Prokaryotes in Kamchatka Hot Springs Evgenii N. Frolov Alexandra V. Gololobova Alexandra A. Klyukina Elizaveta A. Bonch-Osmolovskaya Nikolay V. Pimenov Nikolay A. Chernyh Alexander Y. Merkel 2021-10-01T00:00:00Z https://doi.org/10.3390/microorganisms9102072 https://doaj.org/article/d0dabe4ccf3a47648a92a9701a1304a9 EN eng MDPI AG https://www.mdpi.com/2076-2607/9/10/2072 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms9102072 2076-2607 https://doaj.org/article/d0dabe4ccf3a47648a92a9701a1304a9 Microorganisms, Vol 9, Iss 2072, p 2072 (2021) Kamchatka Peninsula terrestrial hot springs microbial community sulfate-reducing prokaryotes dsrB profiling 16S rRNA taxonomic profiling Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/microorganisms9102072 2022-12-31T07:56:49Z Microbial communities of the Kamchatka Peninsula terrestrial hot springs were studied using radioisotopic and cultural approaches, as well as by the amplification and sequencing of dsrB and 16S rRNA genes fragments. Radioisotopic experiments with 35 S-labeled sulfate showed that microbial communities of the Kamchatka hot springs are actively reducing sulfate. Both the cultivation experiments and the results of dsrB and 16S rRNA genes fragments analyses indicated the presence of microorganisms participating in the reductive part of the sulfur cycle. It was found that sulfate-reducing prokaryotes (SRP) belonging to Desulfobacterota , Nitrospirota and Firmicutes phyla inhabited neutral and slightly acidic hot springs, while bacteria of phylum Thermodesulofobiota preferred moderately acidic hot springs. In high-temperature acidic springs sulfate reduction was mediated by archaea of the phylum Crenarchaeota , chemoorganoheterotrophic representatives of genus Vulcanisaeta being the most probable candidates. The 16S rRNA taxonomic profiling showed that in most of the studied communities SRP was present only as a minor component. Only in one microbial community, the representatives of genus Vulcanisaeta comprised a significant group. Thus, in spite of comparatively low sulfate concentrations in terrestrial hot springs of the Kamchatka, phylogenetically and metabolically diverse groups of sulfate-reducing prokaryotes are operating there coupling carbon and sulfur cycles in these habitats. Article in Journal/Newspaper Kamchatka Kamchatka Peninsula Directory of Open Access Journals: DOAJ Articles Kamchatka Peninsula ENVELOPE(160.000,160.000,56.000,56.000) Microorganisms 9 10 2072 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Kamchatka Peninsula terrestrial hot springs microbial community sulfate-reducing prokaryotes dsrB profiling 16S rRNA taxonomic profiling Biology (General) QH301-705.5 |
spellingShingle |
Kamchatka Peninsula terrestrial hot springs microbial community sulfate-reducing prokaryotes dsrB profiling 16S rRNA taxonomic profiling Biology (General) QH301-705.5 Evgenii N. Frolov Alexandra V. Gololobova Alexandra A. Klyukina Elizaveta A. Bonch-Osmolovskaya Nikolay V. Pimenov Nikolay A. Chernyh Alexander Y. Merkel Diversity and Activity of Sulfate-Reducing Prokaryotes in Kamchatka Hot Springs |
topic_facet |
Kamchatka Peninsula terrestrial hot springs microbial community sulfate-reducing prokaryotes dsrB profiling 16S rRNA taxonomic profiling Biology (General) QH301-705.5 |
description |
Microbial communities of the Kamchatka Peninsula terrestrial hot springs were studied using radioisotopic and cultural approaches, as well as by the amplification and sequencing of dsrB and 16S rRNA genes fragments. Radioisotopic experiments with 35 S-labeled sulfate showed that microbial communities of the Kamchatka hot springs are actively reducing sulfate. Both the cultivation experiments and the results of dsrB and 16S rRNA genes fragments analyses indicated the presence of microorganisms participating in the reductive part of the sulfur cycle. It was found that sulfate-reducing prokaryotes (SRP) belonging to Desulfobacterota , Nitrospirota and Firmicutes phyla inhabited neutral and slightly acidic hot springs, while bacteria of phylum Thermodesulofobiota preferred moderately acidic hot springs. In high-temperature acidic springs sulfate reduction was mediated by archaea of the phylum Crenarchaeota , chemoorganoheterotrophic representatives of genus Vulcanisaeta being the most probable candidates. The 16S rRNA taxonomic profiling showed that in most of the studied communities SRP was present only as a minor component. Only in one microbial community, the representatives of genus Vulcanisaeta comprised a significant group. Thus, in spite of comparatively low sulfate concentrations in terrestrial hot springs of the Kamchatka, phylogenetically and metabolically diverse groups of sulfate-reducing prokaryotes are operating there coupling carbon and sulfur cycles in these habitats. |
format |
Article in Journal/Newspaper |
author |
Evgenii N. Frolov Alexandra V. Gololobova Alexandra A. Klyukina Elizaveta A. Bonch-Osmolovskaya Nikolay V. Pimenov Nikolay A. Chernyh Alexander Y. Merkel |
author_facet |
Evgenii N. Frolov Alexandra V. Gololobova Alexandra A. Klyukina Elizaveta A. Bonch-Osmolovskaya Nikolay V. Pimenov Nikolay A. Chernyh Alexander Y. Merkel |
author_sort |
Evgenii N. Frolov |
title |
Diversity and Activity of Sulfate-Reducing Prokaryotes in Kamchatka Hot Springs |
title_short |
Diversity and Activity of Sulfate-Reducing Prokaryotes in Kamchatka Hot Springs |
title_full |
Diversity and Activity of Sulfate-Reducing Prokaryotes in Kamchatka Hot Springs |
title_fullStr |
Diversity and Activity of Sulfate-Reducing Prokaryotes in Kamchatka Hot Springs |
title_full_unstemmed |
Diversity and Activity of Sulfate-Reducing Prokaryotes in Kamchatka Hot Springs |
title_sort |
diversity and activity of sulfate-reducing prokaryotes in kamchatka hot springs |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/microorganisms9102072 https://doaj.org/article/d0dabe4ccf3a47648a92a9701a1304a9 |
long_lat |
ENVELOPE(160.000,160.000,56.000,56.000) |
geographic |
Kamchatka Peninsula |
geographic_facet |
Kamchatka Peninsula |
genre |
Kamchatka Kamchatka Peninsula |
genre_facet |
Kamchatka Kamchatka Peninsula |
op_source |
Microorganisms, Vol 9, Iss 2072, p 2072 (2021) |
op_relation |
https://www.mdpi.com/2076-2607/9/10/2072 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms9102072 2076-2607 https://doaj.org/article/d0dabe4ccf3a47648a92a9701a1304a9 |
op_doi |
https://doi.org/10.3390/microorganisms9102072 |
container_title |
Microorganisms |
container_volume |
9 |
container_issue |
10 |
container_start_page |
2072 |
_version_ |
1766050496079986688 |