Recombinant protein production in Pseudoalteromonas haloplanktis TAC125 biofilm
Biofilms have great potential for producing valuable products, and recent research has been performed on biofilms for the production of compounds with biotechnological and industrial relevance. However, the production of recombinant proteins using this system is still limited. The recombinant protei...
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ftdoajarticles:oai:doaj.org/article:d0b0c0300fc749daa7d80d34b7fd03c2 2024-09-15T17:42:06+00:00 Recombinant protein production in Pseudoalteromonas haloplanktis TAC125 biofilm Marzia Calvanese Caterina D'Angelo Concetta Lauro Maria Luisa Tutino Ermenegilda Parrilli 2024-06-01T00:00:00Z https://doi.org/10.1016/j.bioflm.2024.100179 https://doaj.org/article/d0b0c0300fc749daa7d80d34b7fd03c2 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2590207524000042 https://doaj.org/toc/2590-2075 2590-2075 doi:10.1016/j.bioflm.2024.100179 https://doaj.org/article/d0b0c0300fc749daa7d80d34b7fd03c2 Biofilm, Vol 7, Iss , Pp 100179- (2024) Biofilm Pseudoalteromonas haloplanktis TAC125 Recombinant protein production Cold-adapted bacteria m-Scarlet GFP Biotechnology TP248.13-248.65 Microbiology QR1-502 article 2024 ftdoajarticles https://doi.org/10.1016/j.bioflm.2024.100179 2024-08-05T17:49:11Z Biofilms have great potential for producing valuable products, and recent research has been performed on biofilms for the production of compounds with biotechnological and industrial relevance. However, the production of recombinant proteins using this system is still limited. The recombinant protein production in microbial hosts is a well-established technology and a variety of expression systems are available. Nevertheless, the production of some recombinant proteins can result in proteolyzed, insoluble, and non-functional forms, therefore it is necessary to start the exploration of non-conventional production systems that, in the future, could be helpful to produce some “difficult” proteins. Non-conventional production systems can be based on the use of alternative hosts and/or on non-conventional ways to grow recombinant cells. In this paper, the use of the Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125 grown in biofilm conditions was explored to produce two fluorescent proteins, GFP and mScarlet. The best conditions for the production were identified by working on media composition, and induction conditions, and by building a new expression vector suitable for the biofilm conditions. Results reported demonstrated that the optimized system for the recombinant protein production in biofilm, although it takes longer than planktonic production, has the same potentiality as the classical planktonic approach with additional advantages since it needs a lower concentration of the carbon sources and doesn't require antibiotic addition. Moreover, in the case of mScarlet, the production in biofilm outperforms the planktonic system in terms of a better quality of the recombinant product. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Biofilm 7 100179 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Biofilm Pseudoalteromonas haloplanktis TAC125 Recombinant protein production Cold-adapted bacteria m-Scarlet GFP Biotechnology TP248.13-248.65 Microbiology QR1-502 |
spellingShingle |
Biofilm Pseudoalteromonas haloplanktis TAC125 Recombinant protein production Cold-adapted bacteria m-Scarlet GFP Biotechnology TP248.13-248.65 Microbiology QR1-502 Marzia Calvanese Caterina D'Angelo Concetta Lauro Maria Luisa Tutino Ermenegilda Parrilli Recombinant protein production in Pseudoalteromonas haloplanktis TAC125 biofilm |
topic_facet |
Biofilm Pseudoalteromonas haloplanktis TAC125 Recombinant protein production Cold-adapted bacteria m-Scarlet GFP Biotechnology TP248.13-248.65 Microbiology QR1-502 |
description |
Biofilms have great potential for producing valuable products, and recent research has been performed on biofilms for the production of compounds with biotechnological and industrial relevance. However, the production of recombinant proteins using this system is still limited. The recombinant protein production in microbial hosts is a well-established technology and a variety of expression systems are available. Nevertheless, the production of some recombinant proteins can result in proteolyzed, insoluble, and non-functional forms, therefore it is necessary to start the exploration of non-conventional production systems that, in the future, could be helpful to produce some “difficult” proteins. Non-conventional production systems can be based on the use of alternative hosts and/or on non-conventional ways to grow recombinant cells. In this paper, the use of the Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125 grown in biofilm conditions was explored to produce two fluorescent proteins, GFP and mScarlet. The best conditions for the production were identified by working on media composition, and induction conditions, and by building a new expression vector suitable for the biofilm conditions. Results reported demonstrated that the optimized system for the recombinant protein production in biofilm, although it takes longer than planktonic production, has the same potentiality as the classical planktonic approach with additional advantages since it needs a lower concentration of the carbon sources and doesn't require antibiotic addition. Moreover, in the case of mScarlet, the production in biofilm outperforms the planktonic system in terms of a better quality of the recombinant product. |
format |
Article in Journal/Newspaper |
author |
Marzia Calvanese Caterina D'Angelo Concetta Lauro Maria Luisa Tutino Ermenegilda Parrilli |
author_facet |
Marzia Calvanese Caterina D'Angelo Concetta Lauro Maria Luisa Tutino Ermenegilda Parrilli |
author_sort |
Marzia Calvanese |
title |
Recombinant protein production in Pseudoalteromonas haloplanktis TAC125 biofilm |
title_short |
Recombinant protein production in Pseudoalteromonas haloplanktis TAC125 biofilm |
title_full |
Recombinant protein production in Pseudoalteromonas haloplanktis TAC125 biofilm |
title_fullStr |
Recombinant protein production in Pseudoalteromonas haloplanktis TAC125 biofilm |
title_full_unstemmed |
Recombinant protein production in Pseudoalteromonas haloplanktis TAC125 biofilm |
title_sort |
recombinant protein production in pseudoalteromonas haloplanktis tac125 biofilm |
publisher |
Elsevier |
publishDate |
2024 |
url |
https://doi.org/10.1016/j.bioflm.2024.100179 https://doaj.org/article/d0b0c0300fc749daa7d80d34b7fd03c2 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Biofilm, Vol 7, Iss , Pp 100179- (2024) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2590207524000042 https://doaj.org/toc/2590-2075 2590-2075 doi:10.1016/j.bioflm.2024.100179 https://doaj.org/article/d0b0c0300fc749daa7d80d34b7fd03c2 |
op_doi |
https://doi.org/10.1016/j.bioflm.2024.100179 |
container_title |
Biofilm |
container_volume |
7 |
container_start_page |
100179 |
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1810488510577115136 |