Colony formation in Phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry
Phaeocystis antarctica is an important phytoplankter of the Ross Sea where it dominates the early season bloom after sea ice retreat and is a major contributor to carbon export. The factors that influence Phaeocystis colony formation and the resultant Ross Sea bloom initiation have been of great sci...
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ftdoajarticles:oai:doaj.org/article:d09fc63362ed499e87e2ead808208b79 2023-05-15T14:04:44+02:00 Colony formation in Phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry S. J. Bender D. M. Moran M. R. McIlvin H. Zheng J. P. McCrow J. Badger G. R. DiTullio A. E. Allen M. A. Saito 2018-08-01T00:00:00Z https://doi.org/10.5194/bg-15-4923-2018 https://doaj.org/article/d09fc63362ed499e87e2ead808208b79 EN eng Copernicus Publications https://www.biogeosciences.net/15/4923/2018/bg-15-4923-2018.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-15-4923-2018 1726-4170 1726-4189 https://doaj.org/article/d09fc63362ed499e87e2ead808208b79 Biogeosciences, Vol 15, Pp 4923-4942 (2018) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2018 ftdoajarticles https://doi.org/10.5194/bg-15-4923-2018 2022-12-31T03:20:26Z Phaeocystis antarctica is an important phytoplankter of the Ross Sea where it dominates the early season bloom after sea ice retreat and is a major contributor to carbon export. The factors that influence Phaeocystis colony formation and the resultant Ross Sea bloom initiation have been of great scientific interest, yet there is little known about the underlying mechanisms responsible for these phenomena. Here, we present laboratory and field studies on Phaeocystis antarctica grown under multiple iron conditions using a coupled proteomic and transcriptomic approach. P. antarctica had a lower iron limitation threshold than a Ross Sea diatom Chaetoceros sp., and at increased iron nutrition (> 120 pM Fe') a shift from flagellate cells to a majority of colonial cells in P. antarctica was observed, implying a role for iron as a trigger for colony formation. Proteome analysis revealed an extensive and coordinated shift in proteome structure linked to iron availability and life cycle transitions with 327 and 436 proteins measured as significantly different between low and high iron in strains 1871 and 1374, respectively. The enzymes flavodoxin and plastocyanin that can functionally replace iron metalloenzymes were observed at low iron treatments consistent with cellular iron-sparing strategies, with plastocyanin having a larger dynamic range. The numerous isoforms of the putative iron-starvation-induced protein (ISIP) group (ISIP2A and ISIP3) had abundance patterns coinciding with that of either low or high iron (and coincident flagellate or the colonial cell types in strain 1871), implying that there may be specific iron acquisition systems for each life cycle type. The proteome analysis also revealed numerous structural proteins associated with each cell type: within flagellate cells actin and tubulin from flagella and haptonema structures as well as a suite of calcium-binding proteins with EF domains were observed. In the colony-dominated samples a variety of structural proteins were observed that are also often ... Article in Journal/Newspaper Antarc* Antarctica Ross Sea Sea ice Directory of Open Access Journals: DOAJ Articles Ross Sea Biogeosciences 15 16 4923 4942 |
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language |
English |
topic |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
spellingShingle |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 S. J. Bender D. M. Moran M. R. McIlvin H. Zheng J. P. McCrow J. Badger G. R. DiTullio A. E. Allen M. A. Saito Colony formation in Phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
Phaeocystis antarctica is an important phytoplankter of the Ross Sea where it dominates the early season bloom after sea ice retreat and is a major contributor to carbon export. The factors that influence Phaeocystis colony formation and the resultant Ross Sea bloom initiation have been of great scientific interest, yet there is little known about the underlying mechanisms responsible for these phenomena. Here, we present laboratory and field studies on Phaeocystis antarctica grown under multiple iron conditions using a coupled proteomic and transcriptomic approach. P. antarctica had a lower iron limitation threshold than a Ross Sea diatom Chaetoceros sp., and at increased iron nutrition (> 120 pM Fe') a shift from flagellate cells to a majority of colonial cells in P. antarctica was observed, implying a role for iron as a trigger for colony formation. Proteome analysis revealed an extensive and coordinated shift in proteome structure linked to iron availability and life cycle transitions with 327 and 436 proteins measured as significantly different between low and high iron in strains 1871 and 1374, respectively. The enzymes flavodoxin and plastocyanin that can functionally replace iron metalloenzymes were observed at low iron treatments consistent with cellular iron-sparing strategies, with plastocyanin having a larger dynamic range. The numerous isoforms of the putative iron-starvation-induced protein (ISIP) group (ISIP2A and ISIP3) had abundance patterns coinciding with that of either low or high iron (and coincident flagellate or the colonial cell types in strain 1871), implying that there may be specific iron acquisition systems for each life cycle type. The proteome analysis also revealed numerous structural proteins associated with each cell type: within flagellate cells actin and tubulin from flagella and haptonema structures as well as a suite of calcium-binding proteins with EF domains were observed. In the colony-dominated samples a variety of structural proteins were observed that are also often ... |
format |
Article in Journal/Newspaper |
author |
S. J. Bender D. M. Moran M. R. McIlvin H. Zheng J. P. McCrow J. Badger G. R. DiTullio A. E. Allen M. A. Saito |
author_facet |
S. J. Bender D. M. Moran M. R. McIlvin H. Zheng J. P. McCrow J. Badger G. R. DiTullio A. E. Allen M. A. Saito |
author_sort |
S. J. Bender |
title |
Colony formation in Phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry |
title_short |
Colony formation in Phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry |
title_full |
Colony formation in Phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry |
title_fullStr |
Colony formation in Phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry |
title_full_unstemmed |
Colony formation in Phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry |
title_sort |
colony formation in phaeocystis antarctica : connecting molecular mechanisms with iron biogeochemistry |
publisher |
Copernicus Publications |
publishDate |
2018 |
url |
https://doi.org/10.5194/bg-15-4923-2018 https://doaj.org/article/d09fc63362ed499e87e2ead808208b79 |
geographic |
Ross Sea |
geographic_facet |
Ross Sea |
genre |
Antarc* Antarctica Ross Sea Sea ice |
genre_facet |
Antarc* Antarctica Ross Sea Sea ice |
op_source |
Biogeosciences, Vol 15, Pp 4923-4942 (2018) |
op_relation |
https://www.biogeosciences.net/15/4923/2018/bg-15-4923-2018.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-15-4923-2018 1726-4170 1726-4189 https://doaj.org/article/d09fc63362ed499e87e2ead808208b79 |
op_doi |
https://doi.org/10.5194/bg-15-4923-2018 |
container_title |
Biogeosciences |
container_volume |
15 |
container_issue |
16 |
container_start_page |
4923 |
op_container_end_page |
4942 |
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1766275996617539584 |