Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii
Abatus agassizii is an irregular sea urchin species that inhabits shallow waters of South Georgia and South Shetlands Islands. As a deposit-feeder, A. agassizii nutrition relies on the ingestion of the surrounding sediment in which it lives barely burrowed. Despite the low complexity of its feeding...
Published in: | Frontiers in Microbiology |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2020
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fmicb.2020.00308 https://doaj.org/article/d09316fbe994448da7904aad907ba45b |
id |
ftdoajarticles:oai:doaj.org/article:d09316fbe994448da7904aad907ba45b |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:d09316fbe994448da7904aad907ba45b 2023-05-15T13:51:14+02:00 Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii Guillaume Schwob Léa Cabrol Elie Poulin Julieta Orlando 2020-02-01T00:00:00Z https://doi.org/10.3389/fmicb.2020.00308 https://doaj.org/article/d09316fbe994448da7904aad907ba45b EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fmicb.2020.00308/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2020.00308 https://doaj.org/article/d09316fbe994448da7904aad907ba45b Frontiers in Microbiology, Vol 11 (2020) heart sea urchin deposit-feeder Abatus agassizii gut microbiota core-microbiota keystone Microbiology QR1-502 article 2020 ftdoajarticles https://doi.org/10.3389/fmicb.2020.00308 2022-12-31T00:19:13Z Abatus agassizii is an irregular sea urchin species that inhabits shallow waters of South Georgia and South Shetlands Islands. As a deposit-feeder, A. agassizii nutrition relies on the ingestion of the surrounding sediment in which it lives barely burrowed. Despite the low complexity of its feeding habit, it harbors a long and twice-looped digestive tract suggesting that it may host a complex bacterial community. Here, we characterized the gut microbiota of specimens from two A. agassizii populations at the south of the King George Island in the West Antarctic Peninsula. Using a metabarcoding approach targeting the 16S rRNA gene, we characterized the Abatus microbiota composition and putative functional capacity, evaluating its differentiation among the gut content and the gut tissue in comparison with the external sediment. Additionally, we aimed to define a core gut microbiota between A. agassizii populations to identify potential keystone bacterial taxa. Our results show that the diversity and the composition of the microbiota, at both genetic and predicted functional levels, were mostly driven by the sample type, and to a lesser extent by the population location. Specific bacterial taxa, belonging mostly to Planctomycetacia and Spirochaetia, were differently enriched in the gut content and the gut tissue, respectively. Predictive functional profiles revealed higher abundance of specific pathways, as the sulfur cycle in the gut content and the amino acid metabolism, in the gut tissue. Further, the definition of a core microbiota allowed to obtain evidence of specific localization of bacterial taxa and the identification of potential keystone taxa assigned to the Desulfobacula and Spirochaeta genera as potentially host selected. The ecological relevance of these keystone taxa in the host metabolism is discussed. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula King George Island Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Antarctic Peninsula King George Island Frontiers in Microbiology 11 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
heart sea urchin deposit-feeder Abatus agassizii gut microbiota core-microbiota keystone Microbiology QR1-502 |
spellingShingle |
heart sea urchin deposit-feeder Abatus agassizii gut microbiota core-microbiota keystone Microbiology QR1-502 Guillaume Schwob Léa Cabrol Elie Poulin Julieta Orlando Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
topic_facet |
heart sea urchin deposit-feeder Abatus agassizii gut microbiota core-microbiota keystone Microbiology QR1-502 |
description |
Abatus agassizii is an irregular sea urchin species that inhabits shallow waters of South Georgia and South Shetlands Islands. As a deposit-feeder, A. agassizii nutrition relies on the ingestion of the surrounding sediment in which it lives barely burrowed. Despite the low complexity of its feeding habit, it harbors a long and twice-looped digestive tract suggesting that it may host a complex bacterial community. Here, we characterized the gut microbiota of specimens from two A. agassizii populations at the south of the King George Island in the West Antarctic Peninsula. Using a metabarcoding approach targeting the 16S rRNA gene, we characterized the Abatus microbiota composition and putative functional capacity, evaluating its differentiation among the gut content and the gut tissue in comparison with the external sediment. Additionally, we aimed to define a core gut microbiota between A. agassizii populations to identify potential keystone bacterial taxa. Our results show that the diversity and the composition of the microbiota, at both genetic and predicted functional levels, were mostly driven by the sample type, and to a lesser extent by the population location. Specific bacterial taxa, belonging mostly to Planctomycetacia and Spirochaetia, were differently enriched in the gut content and the gut tissue, respectively. Predictive functional profiles revealed higher abundance of specific pathways, as the sulfur cycle in the gut content and the amino acid metabolism, in the gut tissue. Further, the definition of a core microbiota allowed to obtain evidence of specific localization of bacterial taxa and the identification of potential keystone taxa assigned to the Desulfobacula and Spirochaeta genera as potentially host selected. The ecological relevance of these keystone taxa in the host metabolism is discussed. |
format |
Article in Journal/Newspaper |
author |
Guillaume Schwob Léa Cabrol Elie Poulin Julieta Orlando |
author_facet |
Guillaume Schwob Léa Cabrol Elie Poulin Julieta Orlando |
author_sort |
Guillaume Schwob |
title |
Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
title_short |
Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
title_full |
Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
title_fullStr |
Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
title_full_unstemmed |
Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
title_sort |
characterization of the gut microbiota of the antarctic heart urchin (spatangoida) abatus agassizii |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmicb.2020.00308 https://doaj.org/article/d09316fbe994448da7904aad907ba45b |
geographic |
Antarctic The Antarctic Antarctic Peninsula King George Island |
geographic_facet |
Antarctic The Antarctic Antarctic Peninsula King George Island |
genre |
Antarc* Antarctic Antarctic Peninsula King George Island |
genre_facet |
Antarc* Antarctic Antarctic Peninsula King George Island |
op_source |
Frontiers in Microbiology, Vol 11 (2020) |
op_relation |
https://www.frontiersin.org/article/10.3389/fmicb.2020.00308/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2020.00308 https://doaj.org/article/d09316fbe994448da7904aad907ba45b |
op_doi |
https://doi.org/10.3389/fmicb.2020.00308 |
container_title |
Frontiers in Microbiology |
container_volume |
11 |
_version_ |
1766255002589855744 |