Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks
The tick-borne encephalitis virus (TBEV) strain Lazo MP36 was isolated from the pool of mosquitoes Aedes vexans collected in Lazo region of Khabarovsk territory in August 2014. Phylogenetic analysis of the strain Lazo MP36 complete genome (GenBank accession number KT001073) revealed its corresponden...
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ftdoajarticles:oai:doaj.org/article:d054508b778c40dd95a867b4561437fa 2023-07-30T04:06:35+02:00 Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks N. M. Pukhovskaya O. V. Morozova N. B. Belozerova S. V. Bakhmetyeva N. P. Vysochina N. I. Zdanovskaya L. I. Ivanov 2017-02-01T00:00:00Z https://doi.org/10.18821/0507-4088-2017-62-1-30-35 https://doaj.org/article/d054508b778c40dd95a867b4561437fa EN RU eng rus Central Research Institute for Epidemiology https://virusjour.crie.ru/jour/article/viewFile/126/46 https://doaj.org/toc/0507-4088 https://doaj.org/toc/2411-2097 0507-4088 2411-2097 doi:10.18821/0507-4088-2017-62-1-30-35 https://doaj.org/article/d054508b778c40dd95a867b4561437fa Вопросы вирусологии, Vol 62, Iss 1, Pp 30-35 (2017) tick-borne and mosquito-borne flaviviruses phylogenetic analysis of complete coding regions of genomes fusion peptide untranslated regions cyclization sequences Microbiology QR1-502 article 2017 ftdoajarticles https://doi.org/10.18821/0507-4088-2017-62-1-30-35 2023-07-16T00:36:07Z The tick-borne encephalitis virus (TBEV) strain Lazo MP36 was isolated from the pool of mosquitoes Aedes vexans collected in Lazo region of Khabarovsk territory in August 2014. Phylogenetic analysis of the strain Lazo MP36 complete genome (GenBank accession number KT001073) revealed its correspondence to the TBEV Far Eastern subtype and differences from the following strains: 1) from ticks Ixodes persulcatus P. Schulze, 1930 [vaccine strain 205 (JX498939) and strains Khekhtzir 1230 (KF880805), Chichagovka (KP844724), Birobidzhan 1354 (KF880805) isolated in 2012-2013]; 2) from mosquitoes [strain Malyshevo (KJ744034) isolated in 1978 from Aedes vexans nipponii in Khabarovsk territory; strain Sakhalin 6-11 isolated from the pool of mosquitoes in 2011 (KF826916)]; 3) from human brain [vaccine strain Sofjin (JN229223), Glubinnoe/2004(DQ862460). Kavalerovo (DQ862460), Svetlogorie (DQ862460)]. The fusion peptide necessary for flavivirus entry to cells of the three TBEV strains isolated from mosquitoes (Lazo MP36, Malyshevo and Sakhalin 6-11) has the canonical structure 98-DRGWGNHCGLFGKGSI-113 for the tick-borne flaviviruses. Amino acid transition H104G typical for the mosquito-borne flaviviruses was not found. Structures of 5’- and 3’-untranslated (UTR) regions of the TBEV strains from mosquitoes were 85-98% homologous to the TBEV strains of all subtypes without recombination with mosquito-borne flaviviruses found in the Far East of Russia. Secondary structures of 5’- and 3'-UTR as well as cyclization sequences (CS) of types a and B are highly homologous for all TBEV isolates independently of the biological hosts and vectors. similarity of the genomes of the TBEV isolates from mosquitoes, ticks and patients as well as pathogenicity of the isolates for new-borne laboratory mice and tissue cultures might suggest a possible role of mosquitoes in the TBEV circulation in natural foci as an accidental or additional virus carrier. Article in Journal/Newspaper Sakhalin Directory of Open Access Journals: DOAJ Articles Lazo ENVELOPE(136.518,136.518,66.608,66.608) Problems of Virology 62 1 30 35 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Russian |
topic |
tick-borne and mosquito-borne flaviviruses phylogenetic analysis of complete coding regions of genomes fusion peptide untranslated regions cyclization sequences Microbiology QR1-502 |
spellingShingle |
tick-borne and mosquito-borne flaviviruses phylogenetic analysis of complete coding regions of genomes fusion peptide untranslated regions cyclization sequences Microbiology QR1-502 N. M. Pukhovskaya O. V. Morozova N. B. Belozerova S. V. Bakhmetyeva N. P. Vysochina N. I. Zdanovskaya L. I. Ivanov Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks |
topic_facet |
tick-borne and mosquito-borne flaviviruses phylogenetic analysis of complete coding regions of genomes fusion peptide untranslated regions cyclization sequences Microbiology QR1-502 |
description |
The tick-borne encephalitis virus (TBEV) strain Lazo MP36 was isolated from the pool of mosquitoes Aedes vexans collected in Lazo region of Khabarovsk territory in August 2014. Phylogenetic analysis of the strain Lazo MP36 complete genome (GenBank accession number KT001073) revealed its correspondence to the TBEV Far Eastern subtype and differences from the following strains: 1) from ticks Ixodes persulcatus P. Schulze, 1930 [vaccine strain 205 (JX498939) and strains Khekhtzir 1230 (KF880805), Chichagovka (KP844724), Birobidzhan 1354 (KF880805) isolated in 2012-2013]; 2) from mosquitoes [strain Malyshevo (KJ744034) isolated in 1978 from Aedes vexans nipponii in Khabarovsk territory; strain Sakhalin 6-11 isolated from the pool of mosquitoes in 2011 (KF826916)]; 3) from human brain [vaccine strain Sofjin (JN229223), Glubinnoe/2004(DQ862460). Kavalerovo (DQ862460), Svetlogorie (DQ862460)]. The fusion peptide necessary for flavivirus entry to cells of the three TBEV strains isolated from mosquitoes (Lazo MP36, Malyshevo and Sakhalin 6-11) has the canonical structure 98-DRGWGNHCGLFGKGSI-113 for the tick-borne flaviviruses. Amino acid transition H104G typical for the mosquito-borne flaviviruses was not found. Structures of 5’- and 3’-untranslated (UTR) regions of the TBEV strains from mosquitoes were 85-98% homologous to the TBEV strains of all subtypes without recombination with mosquito-borne flaviviruses found in the Far East of Russia. Secondary structures of 5’- and 3'-UTR as well as cyclization sequences (CS) of types a and B are highly homologous for all TBEV isolates independently of the biological hosts and vectors. similarity of the genomes of the TBEV isolates from mosquitoes, ticks and patients as well as pathogenicity of the isolates for new-borne laboratory mice and tissue cultures might suggest a possible role of mosquitoes in the TBEV circulation in natural foci as an accidental or additional virus carrier. |
format |
Article in Journal/Newspaper |
author |
N. M. Pukhovskaya O. V. Morozova N. B. Belozerova S. V. Bakhmetyeva N. P. Vysochina N. I. Zdanovskaya L. I. Ivanov |
author_facet |
N. M. Pukhovskaya O. V. Morozova N. B. Belozerova S. V. Bakhmetyeva N. P. Vysochina N. I. Zdanovskaya L. I. Ivanov |
author_sort |
N. M. Pukhovskaya |
title |
Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks |
title_short |
Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks |
title_full |
Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks |
title_fullStr |
Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks |
title_full_unstemmed |
Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks |
title_sort |
comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks |
publisher |
Central Research Institute for Epidemiology |
publishDate |
2017 |
url |
https://doi.org/10.18821/0507-4088-2017-62-1-30-35 https://doaj.org/article/d054508b778c40dd95a867b4561437fa |
long_lat |
ENVELOPE(136.518,136.518,66.608,66.608) |
geographic |
Lazo |
geographic_facet |
Lazo |
genre |
Sakhalin |
genre_facet |
Sakhalin |
op_source |
Вопросы вирусологии, Vol 62, Iss 1, Pp 30-35 (2017) |
op_relation |
https://virusjour.crie.ru/jour/article/viewFile/126/46 https://doaj.org/toc/0507-4088 https://doaj.org/toc/2411-2097 0507-4088 2411-2097 doi:10.18821/0507-4088-2017-62-1-30-35 https://doaj.org/article/d054508b778c40dd95a867b4561437fa |
op_doi |
https://doi.org/10.18821/0507-4088-2017-62-1-30-35 |
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Problems of Virology |
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62 |
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