Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells
IntroductionPurpureocillium lilacinum is emerging as a causal agent of hyalohyphomycosis that is refractory to antifungal drugs; however, the pathogenic mechanisms underlying P. lilacinum infection are not understood. In this study, we investigated the interaction of P. lilacinum conidia with human...
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ftdoajarticles:oai:doaj.org/article:d02b28a0e8e443c3a498b95681d32460 2023-05-15T15:10:44+02:00 Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells Mariana Lima Perazzini Peixoto Dilvani Oliveira Santos Ivy de Castro Campos de Souza Eloah Christina Lyrio Neri Danielly Correa Moreira de Sequeira Paula Mello De Luca Cíntia de Moraes Borba 2014-10-01T00:00:00Z https://doi.org/10.1590/0037-8682-0105-2014 https://doaj.org/article/d02b28a0e8e443c3a498b95681d32460 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000500613&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0105-2014 https://doaj.org/article/d02b28a0e8e443c3a498b95681d32460 Revista da Sociedade Brasileira de Medicina Tropical, Vol 47, Iss 5, Pp 613-617 (2014) Purpureocillium lilacinum Macrophages Dendritic cells Interaction in vitro Arctic medicine. Tropical medicine RC955-962 article 2014 ftdoajarticles https://doi.org/10.1590/0037-8682-0105-2014 2022-12-30T21:27:35Z IntroductionPurpureocillium lilacinum is emerging as a causal agent of hyalohyphomycosis that is refractory to antifungal drugs; however, the pathogenic mechanisms underlying P. lilacinum infection are not understood. In this study, we investigated the interaction of P. lilacinum conidia with human macrophages and dendritic cells in vitro.MethodsSpores of a P. lilacinum clinical isolate were obtained by chill-heat shock. Mononuclear cells were isolated from eight healthy individuals. Monocytes were separated by cold aggregation and differentiated into macrophages by incubation for 7 to 10 days at 37°C or into dendritic cells by the addition of the cytokines human granulocyte-macrophage colony stimulating factor and interleukin-4. Conidial suspension was added to the human cells at 1:1, 2:1, and 5:1 (conidia:cells) ratios for 1h, 6h, and 24h, and the infection was evaluated by Giemsa staining and light microscopy.ResultsAfter 1h interaction, P. lilacinum conidia were internalized by human cells and after 6h contact, some conidia became inflated. After 24h interaction, the conidia produced germ tubes and hyphae, leading to the disruption of macrophage and dendritic cell membranes. The infection rate analyzed after 6h incubation of P. lilacinumconidia with cells at 2:1 and 1:1 ratios was 76.5% and 25.5%, respectively, for macrophages and 54.3% and 19.5%, respectively, for cultured dendritic cells.ConclusionsP. lilacinum conidia are capable of infecting and destroying both macrophages and dendritic cells, clearly demonstrating the ability of this pathogenic fungus to invade human phagocytic cells. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 47 5 613 617 |
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English |
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Purpureocillium lilacinum Macrophages Dendritic cells Interaction in vitro Arctic medicine. Tropical medicine RC955-962 |
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Purpureocillium lilacinum Macrophages Dendritic cells Interaction in vitro Arctic medicine. Tropical medicine RC955-962 Mariana Lima Perazzini Peixoto Dilvani Oliveira Santos Ivy de Castro Campos de Souza Eloah Christina Lyrio Neri Danielly Correa Moreira de Sequeira Paula Mello De Luca Cíntia de Moraes Borba Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells |
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Purpureocillium lilacinum Macrophages Dendritic cells Interaction in vitro Arctic medicine. Tropical medicine RC955-962 |
description |
IntroductionPurpureocillium lilacinum is emerging as a causal agent of hyalohyphomycosis that is refractory to antifungal drugs; however, the pathogenic mechanisms underlying P. lilacinum infection are not understood. In this study, we investigated the interaction of P. lilacinum conidia with human macrophages and dendritic cells in vitro.MethodsSpores of a P. lilacinum clinical isolate were obtained by chill-heat shock. Mononuclear cells were isolated from eight healthy individuals. Monocytes were separated by cold aggregation and differentiated into macrophages by incubation for 7 to 10 days at 37°C or into dendritic cells by the addition of the cytokines human granulocyte-macrophage colony stimulating factor and interleukin-4. Conidial suspension was added to the human cells at 1:1, 2:1, and 5:1 (conidia:cells) ratios for 1h, 6h, and 24h, and the infection was evaluated by Giemsa staining and light microscopy.ResultsAfter 1h interaction, P. lilacinum conidia were internalized by human cells and after 6h contact, some conidia became inflated. After 24h interaction, the conidia produced germ tubes and hyphae, leading to the disruption of macrophage and dendritic cell membranes. The infection rate analyzed after 6h incubation of P. lilacinumconidia with cells at 2:1 and 1:1 ratios was 76.5% and 25.5%, respectively, for macrophages and 54.3% and 19.5%, respectively, for cultured dendritic cells.ConclusionsP. lilacinum conidia are capable of infecting and destroying both macrophages and dendritic cells, clearly demonstrating the ability of this pathogenic fungus to invade human phagocytic cells. |
format |
Article in Journal/Newspaper |
author |
Mariana Lima Perazzini Peixoto Dilvani Oliveira Santos Ivy de Castro Campos de Souza Eloah Christina Lyrio Neri Danielly Correa Moreira de Sequeira Paula Mello De Luca Cíntia de Moraes Borba |
author_facet |
Mariana Lima Perazzini Peixoto Dilvani Oliveira Santos Ivy de Castro Campos de Souza Eloah Christina Lyrio Neri Danielly Correa Moreira de Sequeira Paula Mello De Luca Cíntia de Moraes Borba |
author_sort |
Mariana Lima Perazzini Peixoto |
title |
Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells |
title_short |
Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells |
title_full |
Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells |
title_fullStr |
Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells |
title_full_unstemmed |
Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells |
title_sort |
interaction of an opportunistic fungus purpureocillium lilacinum with human macrophages and dendritic cells |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2014 |
url |
https://doi.org/10.1590/0037-8682-0105-2014 https://doaj.org/article/d02b28a0e8e443c3a498b95681d32460 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 47, Iss 5, Pp 613-617 (2014) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000500613&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0105-2014 https://doaj.org/article/d02b28a0e8e443c3a498b95681d32460 |
op_doi |
https://doi.org/10.1590/0037-8682-0105-2014 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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47 |
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613 |
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