Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment.
The dynamics of regulatory T cells in the course of Trypanosoma cruzi infection is still debated. We previously demonstrated that acute murine T. cruzi infection results in an impaired peripheral CD4+Foxp3+ T cell differentiation due to the acquisition of an abnormal Th1-like phenotype and altered f...
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ftdoajarticles:oai:doaj.org/article:cfe5a1f953c44234b3e12f69b41f6472 2023-05-15T15:12:42+02:00 Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment. Florencia Belén González Flavia Calmon-Hamaty Synara Nô Seara Cordeiro Rodrigo Fernández Bussy Silvana Virginia Spinelli Luciano D'Attilio Oscar Bottasso Wilson Savino Vinícius Cotta-de-Almeida Silvina Raquel Villar Ana Rosa Pérez 2016-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004285 https://doaj.org/article/cfe5a1f953c44234b3e12f69b41f6472 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4706328?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004285 https://doaj.org/article/cfe5a1f953c44234b3e12f69b41f6472 PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004285 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004285 2022-12-31T13:31:37Z The dynamics of regulatory T cells in the course of Trypanosoma cruzi infection is still debated. We previously demonstrated that acute murine T. cruzi infection results in an impaired peripheral CD4+Foxp3+ T cell differentiation due to the acquisition of an abnormal Th1-like phenotype and altered functional features, negatively impacting on the course of infection. Moreover, T. cruzi infection induces an intense thymic atrophy. As known, the thymus is the primary lymphoid organ in which thymic-derived regulatory T cells, known as tTregs, differentiate. Considering the lack of available data about the effect of T. cruzi infection upon tTregs, we examined tTreg dynamics during the course of disease. We confirmed that T. cruzi infection induces a marked loss of tTreg cell number associated to cell precursor exhaustion, partially avoided by glucocorticoid ablation- and IL-2 survival factor depletion. At the same time, tTregs accumulate within the CD4 single-positive compartment, exhibiting an increased Ki-67/Annexin V ratio compared to controls. Moreover, tTregs enhance after the infection the expression of signature markers (CD25, CD62L and GITR) and they also display alterations in the expression of migration-associated molecules (α chains of VLAs and chemokine receptors) such as functional fibronectin-driven migratory disturbance. Taken together, we provide data demonstrating profound alterations in tTreg compartment during acute murine T. cruzi infection, denoting that their homeostasis is significantly affected. The evident loss of tTreg cell number may compromise the composition of tTreg peripheral pool, and such sustained alteration over time may be partially related to the immune dysregulation observed in the chronic phase of the disease. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 1 e0004285 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Florencia Belén González Flavia Calmon-Hamaty Synara Nô Seara Cordeiro Rodrigo Fernández Bussy Silvana Virginia Spinelli Luciano D'Attilio Oscar Bottasso Wilson Savino Vinícius Cotta-de-Almeida Silvina Raquel Villar Ana Rosa Pérez Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The dynamics of regulatory T cells in the course of Trypanosoma cruzi infection is still debated. We previously demonstrated that acute murine T. cruzi infection results in an impaired peripheral CD4+Foxp3+ T cell differentiation due to the acquisition of an abnormal Th1-like phenotype and altered functional features, negatively impacting on the course of infection. Moreover, T. cruzi infection induces an intense thymic atrophy. As known, the thymus is the primary lymphoid organ in which thymic-derived regulatory T cells, known as tTregs, differentiate. Considering the lack of available data about the effect of T. cruzi infection upon tTregs, we examined tTreg dynamics during the course of disease. We confirmed that T. cruzi infection induces a marked loss of tTreg cell number associated to cell precursor exhaustion, partially avoided by glucocorticoid ablation- and IL-2 survival factor depletion. At the same time, tTregs accumulate within the CD4 single-positive compartment, exhibiting an increased Ki-67/Annexin V ratio compared to controls. Moreover, tTregs enhance after the infection the expression of signature markers (CD25, CD62L and GITR) and they also display alterations in the expression of migration-associated molecules (α chains of VLAs and chemokine receptors) such as functional fibronectin-driven migratory disturbance. Taken together, we provide data demonstrating profound alterations in tTreg compartment during acute murine T. cruzi infection, denoting that their homeostasis is significantly affected. The evident loss of tTreg cell number may compromise the composition of tTreg peripheral pool, and such sustained alteration over time may be partially related to the immune dysregulation observed in the chronic phase of the disease. |
format |
Article in Journal/Newspaper |
author |
Florencia Belén González Flavia Calmon-Hamaty Synara Nô Seara Cordeiro Rodrigo Fernández Bussy Silvana Virginia Spinelli Luciano D'Attilio Oscar Bottasso Wilson Savino Vinícius Cotta-de-Almeida Silvina Raquel Villar Ana Rosa Pérez |
author_facet |
Florencia Belén González Flavia Calmon-Hamaty Synara Nô Seara Cordeiro Rodrigo Fernández Bussy Silvana Virginia Spinelli Luciano D'Attilio Oscar Bottasso Wilson Savino Vinícius Cotta-de-Almeida Silvina Raquel Villar Ana Rosa Pérez |
author_sort |
Florencia Belén González |
title |
Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment. |
title_short |
Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment. |
title_full |
Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment. |
title_fullStr |
Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment. |
title_full_unstemmed |
Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment. |
title_sort |
trypanosoma cruzi experimental infection impacts on the thymic regulatory t cell compartment. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004285 https://doaj.org/article/cfe5a1f953c44234b3e12f69b41f6472 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004285 (2016) |
op_relation |
http://europepmc.org/articles/PMC4706328?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004285 https://doaj.org/article/cfe5a1f953c44234b3e12f69b41f6472 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004285 |
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PLOS Neglected Tropical Diseases |
container_volume |
10 |
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1 |
container_start_page |
e0004285 |
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