Yellow fever vaccine protects mice against Zika virus infection
Zika virus (ZIKV) emerged as an important infectious disease agent in Brazil in 2016. Infection usually leads to mild symptoms, but severe congenital neurological disorders and Guillain-Barré syndrome have been reported following ZIKV exposure. Creating an effective vaccine against ZIKV is a public...
Main Authors: | , , , , , , , , , , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2021
|
Subjects: | |
Online Access: | https://doaj.org/article/cfd630acb424434ca57acb58b694898d |
id |
ftdoajarticles:oai:doaj.org/article:cfd630acb424434ca57acb58b694898d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:cfd630acb424434ca57acb58b694898d 2023-05-15T15:14:55+02:00 Yellow fever vaccine protects mice against Zika virus infection Ana C. Vicente Santos Francisca H. Guedes-da-Silva Carlos H. Dumard Vivian N. S. Ferreira Igor P. S. da Costa Ruana A. Machado Fernanda G. Q. Barros-Aragão Rômulo L. S. Neris Júlio S. dos-Santos Iranaia Assunção-Miranda Claudia P. Figueiredo André A. Dias Andre M. O. Gomes Herbert L. de Matos Guedes Andrea C. Oliveira Jerson L. Silva 2021-11-01T00:00:00Z https://doaj.org/article/cfd630acb424434ca57acb58b694898d EN eng Public Library of Science (PLoS) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594798/?tool=EBI https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 https://doaj.org/article/cfd630acb424434ca57acb58b694898d PLoS Neglected Tropical Diseases, Vol 15, Iss 11 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles 2022-12-31T09:16:08Z Zika virus (ZIKV) emerged as an important infectious disease agent in Brazil in 2016. Infection usually leads to mild symptoms, but severe congenital neurological disorders and Guillain-Barré syndrome have been reported following ZIKV exposure. Creating an effective vaccine against ZIKV is a public health priority. We describe the protective effect of an already licensed attenuated yellow fever vaccine (YFV, 17DD) in type-I interferon receptor knockout mice (A129) and immunocompetent BALB/c and SV-129 (A129 background) mice infected with ZIKV. YFV vaccination provided protection against ZIKV, with decreased mortality in A129 mice, a reduction in the cerebral viral load in all mice, and weight loss prevention in BALB/c mice. The A129 mice that were challenged two and three weeks after the first dose of the vaccine were fully protected, whereas partial protection was observed five weeks after vaccination. In all cases, the YFV vaccine provoked a substantial decrease in the cerebral viral load. YFV immunization also prevented hippocampal synapse loss and microgliosis in ZIKV-infected mice. Our vaccine model is T cell-dependent, with AG129 mice being unable to tolerate immunization (vaccination is lethal in this mouse model), indicating the importance of IFN-γ in immunogenicity. To confirm the role of T cells, we immunized nude mice that we demonstrated to be very susceptible to infection. Immunization with YFV and challenge 7 days after booster did not protect nude mice in terms of weight loss and showed partial protection in the survival curve. When we evaluated the humoral response, the vaccine elicited significant antibody titers against ZIKV; however, it showed no neutralizing activity in vitro and in vivo. The data indicate that a cell-mediated response promotes protection against cerebral infection, which is crucial to vaccine protection, and it appears to not necessarily require a humoral response. This protective effect can also be attributed to innate factors, but more studies are needed to strengthen this ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Barré ENVELOPE(-68.550,-68.550,-67.500,-67.500) |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ana C. Vicente Santos Francisca H. Guedes-da-Silva Carlos H. Dumard Vivian N. S. Ferreira Igor P. S. da Costa Ruana A. Machado Fernanda G. Q. Barros-Aragão Rômulo L. S. Neris Júlio S. dos-Santos Iranaia Assunção-Miranda Claudia P. Figueiredo André A. Dias Andre M. O. Gomes Herbert L. de Matos Guedes Andrea C. Oliveira Jerson L. Silva Yellow fever vaccine protects mice against Zika virus infection |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Zika virus (ZIKV) emerged as an important infectious disease agent in Brazil in 2016. Infection usually leads to mild symptoms, but severe congenital neurological disorders and Guillain-Barré syndrome have been reported following ZIKV exposure. Creating an effective vaccine against ZIKV is a public health priority. We describe the protective effect of an already licensed attenuated yellow fever vaccine (YFV, 17DD) in type-I interferon receptor knockout mice (A129) and immunocompetent BALB/c and SV-129 (A129 background) mice infected with ZIKV. YFV vaccination provided protection against ZIKV, with decreased mortality in A129 mice, a reduction in the cerebral viral load in all mice, and weight loss prevention in BALB/c mice. The A129 mice that were challenged two and three weeks after the first dose of the vaccine were fully protected, whereas partial protection was observed five weeks after vaccination. In all cases, the YFV vaccine provoked a substantial decrease in the cerebral viral load. YFV immunization also prevented hippocampal synapse loss and microgliosis in ZIKV-infected mice. Our vaccine model is T cell-dependent, with AG129 mice being unable to tolerate immunization (vaccination is lethal in this mouse model), indicating the importance of IFN-γ in immunogenicity. To confirm the role of T cells, we immunized nude mice that we demonstrated to be very susceptible to infection. Immunization with YFV and challenge 7 days after booster did not protect nude mice in terms of weight loss and showed partial protection in the survival curve. When we evaluated the humoral response, the vaccine elicited significant antibody titers against ZIKV; however, it showed no neutralizing activity in vitro and in vivo. The data indicate that a cell-mediated response promotes protection against cerebral infection, which is crucial to vaccine protection, and it appears to not necessarily require a humoral response. This protective effect can also be attributed to innate factors, but more studies are needed to strengthen this ... |
format |
Article in Journal/Newspaper |
author |
Ana C. Vicente Santos Francisca H. Guedes-da-Silva Carlos H. Dumard Vivian N. S. Ferreira Igor P. S. da Costa Ruana A. Machado Fernanda G. Q. Barros-Aragão Rômulo L. S. Neris Júlio S. dos-Santos Iranaia Assunção-Miranda Claudia P. Figueiredo André A. Dias Andre M. O. Gomes Herbert L. de Matos Guedes Andrea C. Oliveira Jerson L. Silva |
author_facet |
Ana C. Vicente Santos Francisca H. Guedes-da-Silva Carlos H. Dumard Vivian N. S. Ferreira Igor P. S. da Costa Ruana A. Machado Fernanda G. Q. Barros-Aragão Rômulo L. S. Neris Júlio S. dos-Santos Iranaia Assunção-Miranda Claudia P. Figueiredo André A. Dias Andre M. O. Gomes Herbert L. de Matos Guedes Andrea C. Oliveira Jerson L. Silva |
author_sort |
Ana C. Vicente Santos |
title |
Yellow fever vaccine protects mice against Zika virus infection |
title_short |
Yellow fever vaccine protects mice against Zika virus infection |
title_full |
Yellow fever vaccine protects mice against Zika virus infection |
title_fullStr |
Yellow fever vaccine protects mice against Zika virus infection |
title_full_unstemmed |
Yellow fever vaccine protects mice against Zika virus infection |
title_sort |
yellow fever vaccine protects mice against zika virus infection |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doaj.org/article/cfd630acb424434ca57acb58b694898d |
long_lat |
ENVELOPE(-68.550,-68.550,-67.500,-67.500) |
geographic |
Arctic Barré |
geographic_facet |
Arctic Barré |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 11 (2021) |
op_relation |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594798/?tool=EBI https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 https://doaj.org/article/cfd630acb424434ca57acb58b694898d |
_version_ |
1766345307464925184 |