Induction of allopurinol resistance in Leishmania infantum isolated from dogs.
Resistance to allopurinol in zoonotic canine leishmaniasis has been recently shown to be associated with disease relapse in naturally-infected dogs. However, information regarding the formation of resistance and its dynamics is lacking. This study describes the successful in-vitro induction of allop...
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ftdoajarticles:oai:doaj.org/article:cf7da8d3c4f44f28917c77eb7e40656c 2023-05-15T15:04:15+02:00 Induction of allopurinol resistance in Leishmania infantum isolated from dogs. Daniel Yasur-Landau Charles L Jaffe Adi Doron-Faigenboim Lior David Gad Baneth 2017-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005910 https://doaj.org/article/cf7da8d3c4f44f28917c77eb7e40656c EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5608428?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005910 https://doaj.org/article/cf7da8d3c4f44f28917c77eb7e40656c PLoS Neglected Tropical Diseases, Vol 11, Iss 9, p e0005910 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005910 2022-12-31T15:19:04Z Resistance to allopurinol in zoonotic canine leishmaniasis has been recently shown to be associated with disease relapse in naturally-infected dogs. However, information regarding the formation of resistance and its dynamics is lacking. This study describes the successful in-vitro induction of allopurinol resistance in Leishmania infantum cultured under increasing drug pressure. Allopurinol susceptibility and growth rate of induced parasites were monitored over 23 weeks and parasite clones were tested at selected time points and compared to their parental lines, both as promastigotes and as amastigotes. Allopurinol resistance was formed in strains from two parasite stocks producing a 20-fold rise in IC50 along three distinct growth phases. In addition, characteristic differential clustering of single nucleotide polymorphisms (SNP) was found in drug sensitive and resistant parasite clones. Results confirm that genetic polymorphism, as well as clonal heterogeneity, contribute to in-vitro resistance to allopurinol, which is likely to occur in natural infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 9 e0005910 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Daniel Yasur-Landau Charles L Jaffe Adi Doron-Faigenboim Lior David Gad Baneth Induction of allopurinol resistance in Leishmania infantum isolated from dogs. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Resistance to allopurinol in zoonotic canine leishmaniasis has been recently shown to be associated with disease relapse in naturally-infected dogs. However, information regarding the formation of resistance and its dynamics is lacking. This study describes the successful in-vitro induction of allopurinol resistance in Leishmania infantum cultured under increasing drug pressure. Allopurinol susceptibility and growth rate of induced parasites were monitored over 23 weeks and parasite clones were tested at selected time points and compared to their parental lines, both as promastigotes and as amastigotes. Allopurinol resistance was formed in strains from two parasite stocks producing a 20-fold rise in IC50 along three distinct growth phases. In addition, characteristic differential clustering of single nucleotide polymorphisms (SNP) was found in drug sensitive and resistant parasite clones. Results confirm that genetic polymorphism, as well as clonal heterogeneity, contribute to in-vitro resistance to allopurinol, which is likely to occur in natural infection. |
format |
Article in Journal/Newspaper |
author |
Daniel Yasur-Landau Charles L Jaffe Adi Doron-Faigenboim Lior David Gad Baneth |
author_facet |
Daniel Yasur-Landau Charles L Jaffe Adi Doron-Faigenboim Lior David Gad Baneth |
author_sort |
Daniel Yasur-Landau |
title |
Induction of allopurinol resistance in Leishmania infantum isolated from dogs. |
title_short |
Induction of allopurinol resistance in Leishmania infantum isolated from dogs. |
title_full |
Induction of allopurinol resistance in Leishmania infantum isolated from dogs. |
title_fullStr |
Induction of allopurinol resistance in Leishmania infantum isolated from dogs. |
title_full_unstemmed |
Induction of allopurinol resistance in Leishmania infantum isolated from dogs. |
title_sort |
induction of allopurinol resistance in leishmania infantum isolated from dogs. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005910 https://doaj.org/article/cf7da8d3c4f44f28917c77eb7e40656c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 9, p e0005910 (2017) |
op_relation |
http://europepmc.org/articles/PMC5608428?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005910 https://doaj.org/article/cf7da8d3c4f44f28917c77eb7e40656c |
op_doi |
https://doi.org/10.1371/journal.pntd.0005910 |
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PLOS Neglected Tropical Diseases |
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11 |
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9 |
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e0005910 |
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1766336053335031808 |