Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study.
The 2013-2016 Ebola virus outbreak in West Africa was the largest and deadliest outbreak to date. Here we conducted a serological study to examine the antibody levels in survivors and the seroconversion in close contacts who took care of Ebola-infected individuals, but did not develop symptoms of Eb...
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ftdoajarticles:oai:doaj.org/article:cf794a1e41354bbc99218be7d59e8ca9 2023-05-15T15:08:31+02:00 Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study. Peter J Halfmann Amie J Eisfeld Tokiko Watanabe Tadashi Maemura Makoto Yamashita Satoshi Fukuyama Tammy Armbrust Isaiah Rozich Alhaji N'jai Gabriele Neumann Yoshihiro Kawaoka Foday Sahr 2019-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007654 https://doaj.org/article/cf794a1e41354bbc99218be7d59e8ca9 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007654 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007654 https://doaj.org/article/cf794a1e41354bbc99218be7d59e8ca9 PLoS Neglected Tropical Diseases, Vol 13, Iss 8, p e0007654 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007654 2022-12-31T09:21:49Z The 2013-2016 Ebola virus outbreak in West Africa was the largest and deadliest outbreak to date. Here we conducted a serological study to examine the antibody levels in survivors and the seroconversion in close contacts who took care of Ebola-infected individuals, but did not develop symptoms of Ebola virus disease. In March 2017, we collected blood samples from 481 individuals in Makeni, Sierra Leone: 214 survivors and 267 close contacts. Using commercial, quantitative ELISAs, we tested the plasma for IgG-specific antibodies against three major viral antigens: GP, the only viral glycoprotein expressed on the virus surface; NP, the most abundant viral protein; and VP40, a major structural protein of Zaire ebolavirus. We also determined neutralizing antibody titers. In the cohort of Ebola survivors, 97.7% of samples (209/214) had measurable antibody levels against GP, NP, and/or VP40. Of these positive samples, all but one had measurable neutralizing antibody titers against Ebola virus. For the close contacts, up to 12.7% (34/267) may have experienced a subclinical virus infection as indicated by detectable antibodies against GP. Further investigation is warranted to determine whether these close contacts truly experienced subclinical infections and whether these asymptomatic infections played a role in the dynamics of transmission. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 8 e0007654 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Peter J Halfmann Amie J Eisfeld Tokiko Watanabe Tadashi Maemura Makoto Yamashita Satoshi Fukuyama Tammy Armbrust Isaiah Rozich Alhaji N'jai Gabriele Neumann Yoshihiro Kawaoka Foday Sahr Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The 2013-2016 Ebola virus outbreak in West Africa was the largest and deadliest outbreak to date. Here we conducted a serological study to examine the antibody levels in survivors and the seroconversion in close contacts who took care of Ebola-infected individuals, but did not develop symptoms of Ebola virus disease. In March 2017, we collected blood samples from 481 individuals in Makeni, Sierra Leone: 214 survivors and 267 close contacts. Using commercial, quantitative ELISAs, we tested the plasma for IgG-specific antibodies against three major viral antigens: GP, the only viral glycoprotein expressed on the virus surface; NP, the most abundant viral protein; and VP40, a major structural protein of Zaire ebolavirus. We also determined neutralizing antibody titers. In the cohort of Ebola survivors, 97.7% of samples (209/214) had measurable antibody levels against GP, NP, and/or VP40. Of these positive samples, all but one had measurable neutralizing antibody titers against Ebola virus. For the close contacts, up to 12.7% (34/267) may have experienced a subclinical virus infection as indicated by detectable antibodies against GP. Further investigation is warranted to determine whether these close contacts truly experienced subclinical infections and whether these asymptomatic infections played a role in the dynamics of transmission. |
format |
Article in Journal/Newspaper |
author |
Peter J Halfmann Amie J Eisfeld Tokiko Watanabe Tadashi Maemura Makoto Yamashita Satoshi Fukuyama Tammy Armbrust Isaiah Rozich Alhaji N'jai Gabriele Neumann Yoshihiro Kawaoka Foday Sahr |
author_facet |
Peter J Halfmann Amie J Eisfeld Tokiko Watanabe Tadashi Maemura Makoto Yamashita Satoshi Fukuyama Tammy Armbrust Isaiah Rozich Alhaji N'jai Gabriele Neumann Yoshihiro Kawaoka Foday Sahr |
author_sort |
Peter J Halfmann |
title |
Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study. |
title_short |
Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study. |
title_full |
Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study. |
title_fullStr |
Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study. |
title_full_unstemmed |
Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study. |
title_sort |
serological analysis of ebola virus survivors and close contacts in sierra leone: a cross-sectional study. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007654 https://doaj.org/article/cf794a1e41354bbc99218be7d59e8ca9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 8, p e0007654 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007654 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007654 https://doaj.org/article/cf794a1e41354bbc99218be7d59e8ca9 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007654 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
8 |
container_start_page |
e0007654 |
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1766339869105192960 |