Dietary Inclusion of Black Soldier Fly (Hermetia Illucens) Larvae Meal and Paste Improved Gut Health but Had Minor Effects on Skin Mucus Proteome and Immune Response in Atlantic Salmon (Salmo Salar)
The present study investigated effects of dietary inclusion of black soldier fly larvae (BSFL) (Hermetia illucens) meal and paste on gut health, plasma biochemical parameters, immune response and skin mucus proteome in pre-smolt Atlantic salmon (Salmo salar). The seven-week experiment consisted of s...
Published in: | Frontiers in Immunology |
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2021
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Online Access: | https://doi.org/10.3389/fimmu.2021.599530 https://doaj.org/article/cf76e3a4adb541738f3097a71633c92c |
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ftdoajarticles:oai:doaj.org/article:cf76e3a4adb541738f3097a71633c92c 2023-05-15T15:31:29+02:00 Dietary Inclusion of Black Soldier Fly (Hermetia Illucens) Larvae Meal and Paste Improved Gut Health but Had Minor Effects on Skin Mucus Proteome and Immune Response in Atlantic Salmon (Salmo Salar) Pabodha Weththasinghe Leidy Lagos Marcos Cortés Jon Øvrum Hansen Margareth Øverland 2021-02-01T00:00:00Z https://doi.org/10.3389/fimmu.2021.599530 https://doaj.org/article/cf76e3a4adb541738f3097a71633c92c EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fimmu.2021.599530/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2021.599530 https://doaj.org/article/cf76e3a4adb541738f3097a71633c92c Frontiers in Immunology, Vol 12 (2021) black soldier fly larvae meal paste Atlantic salmon gut health plasma biochemical parameters Immunologic diseases. Allergy RC581-607 article 2021 ftdoajarticles https://doi.org/10.3389/fimmu.2021.599530 2022-12-31T04:07:36Z The present study investigated effects of dietary inclusion of black soldier fly larvae (BSFL) (Hermetia illucens) meal and paste on gut health, plasma biochemical parameters, immune response and skin mucus proteome in pre-smolt Atlantic salmon (Salmo salar). The seven-week experiment consisted of seven experimental diets: a control diet based on fishmeal and plant protein (Control-1); three BSFL meal diets, substituting 6.25% (6.25IM), 12.5% (12.5IM) and 25% (25IM) of protein; two BSFL paste diets, substituting 3.7% (3.7IP) and 6.7% (6.7IP) of protein and an extra control diet with 0.88% of formic acid (Control-2). The 6.25IM diet reduced enterocyte steatosis in pyloric caeca, improved distal intestine histology, and reduced IgM in distal intestine. The fish fed 12.5IM diet reduced enterocyte steatosis in pyloric caeca, improved distal intestine histology, had a higher plasma lysozyme content compared to 6.25IM, and tend to increase phagocytic activity in head-kidney macrophages-like cells. On the other hand, 25IM diet improved distal intestine histology, but showed mild-moderate enterocyte steatosis in pyloric caeca, increased IFNγ and reduced IgM in distal intestine. In the case of BSFL paste diets, 3.7IP diet caused mild inflammatory changes in distal intestine, although it reduced enterocyte steatosis in pyloric caeca. The 6.7IP diet reduced enterocyte steatosis in pyloric caeca and improved distal intestine histology. Increasing level of BSFL meal in the diet linearly decreased plasma C-reactive protein, whereas increasing level of BSFL paste linearly increased plasma antioxidant capacity. Dietary inclusion of BSFL meal and paste had minor effects on the expression profile of proteins in skin mucus and no effects on immune markers in splenocytes. BSFL meal showed no negative effect on liver and muscle health as indicated by plasma alanine aminotranseferase, asparate aminotransferase and creatine kinase. The present study showed that replacing conventional protein sources with low to moderate levels of BSFL ... Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Frontiers in Immunology 12 |
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Directory of Open Access Journals: DOAJ Articles |
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topic |
black soldier fly larvae meal paste Atlantic salmon gut health plasma biochemical parameters Immunologic diseases. Allergy RC581-607 |
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black soldier fly larvae meal paste Atlantic salmon gut health plasma biochemical parameters Immunologic diseases. Allergy RC581-607 Pabodha Weththasinghe Leidy Lagos Marcos Cortés Jon Øvrum Hansen Margareth Øverland Dietary Inclusion of Black Soldier Fly (Hermetia Illucens) Larvae Meal and Paste Improved Gut Health but Had Minor Effects on Skin Mucus Proteome and Immune Response in Atlantic Salmon (Salmo Salar) |
topic_facet |
black soldier fly larvae meal paste Atlantic salmon gut health plasma biochemical parameters Immunologic diseases. Allergy RC581-607 |
description |
The present study investigated effects of dietary inclusion of black soldier fly larvae (BSFL) (Hermetia illucens) meal and paste on gut health, plasma biochemical parameters, immune response and skin mucus proteome in pre-smolt Atlantic salmon (Salmo salar). The seven-week experiment consisted of seven experimental diets: a control diet based on fishmeal and plant protein (Control-1); three BSFL meal diets, substituting 6.25% (6.25IM), 12.5% (12.5IM) and 25% (25IM) of protein; two BSFL paste diets, substituting 3.7% (3.7IP) and 6.7% (6.7IP) of protein and an extra control diet with 0.88% of formic acid (Control-2). The 6.25IM diet reduced enterocyte steatosis in pyloric caeca, improved distal intestine histology, and reduced IgM in distal intestine. The fish fed 12.5IM diet reduced enterocyte steatosis in pyloric caeca, improved distal intestine histology, had a higher plasma lysozyme content compared to 6.25IM, and tend to increase phagocytic activity in head-kidney macrophages-like cells. On the other hand, 25IM diet improved distal intestine histology, but showed mild-moderate enterocyte steatosis in pyloric caeca, increased IFNγ and reduced IgM in distal intestine. In the case of BSFL paste diets, 3.7IP diet caused mild inflammatory changes in distal intestine, although it reduced enterocyte steatosis in pyloric caeca. The 6.7IP diet reduced enterocyte steatosis in pyloric caeca and improved distal intestine histology. Increasing level of BSFL meal in the diet linearly decreased plasma C-reactive protein, whereas increasing level of BSFL paste linearly increased plasma antioxidant capacity. Dietary inclusion of BSFL meal and paste had minor effects on the expression profile of proteins in skin mucus and no effects on immune markers in splenocytes. BSFL meal showed no negative effect on liver and muscle health as indicated by plasma alanine aminotranseferase, asparate aminotransferase and creatine kinase. The present study showed that replacing conventional protein sources with low to moderate levels of BSFL ... |
format |
Article in Journal/Newspaper |
author |
Pabodha Weththasinghe Leidy Lagos Marcos Cortés Jon Øvrum Hansen Margareth Øverland |
author_facet |
Pabodha Weththasinghe Leidy Lagos Marcos Cortés Jon Øvrum Hansen Margareth Øverland |
author_sort |
Pabodha Weththasinghe |
title |
Dietary Inclusion of Black Soldier Fly (Hermetia Illucens) Larvae Meal and Paste Improved Gut Health but Had Minor Effects on Skin Mucus Proteome and Immune Response in Atlantic Salmon (Salmo Salar) |
title_short |
Dietary Inclusion of Black Soldier Fly (Hermetia Illucens) Larvae Meal and Paste Improved Gut Health but Had Minor Effects on Skin Mucus Proteome and Immune Response in Atlantic Salmon (Salmo Salar) |
title_full |
Dietary Inclusion of Black Soldier Fly (Hermetia Illucens) Larvae Meal and Paste Improved Gut Health but Had Minor Effects on Skin Mucus Proteome and Immune Response in Atlantic Salmon (Salmo Salar) |
title_fullStr |
Dietary Inclusion of Black Soldier Fly (Hermetia Illucens) Larvae Meal and Paste Improved Gut Health but Had Minor Effects on Skin Mucus Proteome and Immune Response in Atlantic Salmon (Salmo Salar) |
title_full_unstemmed |
Dietary Inclusion of Black Soldier Fly (Hermetia Illucens) Larvae Meal and Paste Improved Gut Health but Had Minor Effects on Skin Mucus Proteome and Immune Response in Atlantic Salmon (Salmo Salar) |
title_sort |
dietary inclusion of black soldier fly (hermetia illucens) larvae meal and paste improved gut health but had minor effects on skin mucus proteome and immune response in atlantic salmon (salmo salar) |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fimmu.2021.599530 https://doaj.org/article/cf76e3a4adb541738f3097a71633c92c |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Frontiers in Immunology, Vol 12 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fimmu.2021.599530/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2021.599530 https://doaj.org/article/cf76e3a4adb541738f3097a71633c92c |
op_doi |
https://doi.org/10.3389/fimmu.2021.599530 |
container_title |
Frontiers in Immunology |
container_volume |
12 |
_version_ |
1766361994588323840 |