Immunomodulatory activity of Alaska pollock hydrolysates obtained by glutamic acid biosensor – Artificial neural network and the identification of its active central fragment
The glutamic acid biosensor and artificial neural network (GLU-ANN) was employed to monitor the hydrolysis of Alaska pollock protein for production of immunomodulatory peptides. The relative error of GLU-ANN was in the range of 0.23% to 2.81%. The target pollock hydrolysates (PFH) were prepared usin...
Published in: | Journal of Functional Foods |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2016
|
Subjects: | |
Online Access: | https://doi.org/10.1016/j.jff.2016.03.033 https://doaj.org/article/cf50e6741c904c9eb6763e20590c3bb9 |
id |
ftdoajarticles:oai:doaj.org/article:cf50e6741c904c9eb6763e20590c3bb9 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:cf50e6741c904c9eb6763e20590c3bb9 2023-05-15T13:09:20+02:00 Immunomodulatory activity of Alaska pollock hydrolysates obtained by glutamic acid biosensor – Artificial neural network and the identification of its active central fragment Hu Hou Yan Fan Shikai Wang Leilei Si Bafang Li 2016-06-01T00:00:00Z https://doi.org/10.1016/j.jff.2016.03.033 https://doaj.org/article/cf50e6741c904c9eb6763e20590c3bb9 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S1756464616300561 https://doaj.org/toc/1756-4646 1756-4646 doi:10.1016/j.jff.2016.03.033 https://doaj.org/article/cf50e6741c904c9eb6763e20590c3bb9 Journal of Functional Foods, Vol 24, Iss , Pp 37-47 (2016) Hydrolysates Immunomodulatory peptide Purification Amino acid sequence Enzymatic hydrolysis Nutrition. Foods and food supply TX341-641 article 2016 ftdoajarticles https://doi.org/10.1016/j.jff.2016.03.033 2022-12-31T15:50:30Z The glutamic acid biosensor and artificial neural network (GLU-ANN) was employed to monitor the hydrolysis of Alaska pollock protein for production of immunomodulatory peptides. The relative error of GLU-ANN was in the range of 0.23% to 2.81%. The target pollock hydrolysates (PFH) were prepared using GLU-ANN, which significantly enhanced humoral, cellular, and non-specific immunity in immunosuppressed mice, induced by hydrogenated cortisone (p < 0.05). In addition, treatment with PFH significantly increased the production of interleukin (IL)-2, IL-4, and IL-6 in immunosuppressed mice (p < 0.05), while interferon-gamma (IFN-γ) showed no significant change. The active central fragment that exhibited the highest lymphocyte proliferation rates was further purified using ion exchange chromatography, gel filtration chromatography, and reversed-phase high performance liquid chromatography (RP-HPLC). The amino acid sequence of this fragment was as follows: Pro-Thr-Gly-Ala-Asp-Tyr (PTGADY). Therefore, PFH shows promise as a potent immunomodulator with potential applications in functional food. Article in Journal/Newspaper alaska pollock Alaska Directory of Open Access Journals: DOAJ Articles Journal of Functional Foods 24 37 47 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Hydrolysates Immunomodulatory peptide Purification Amino acid sequence Enzymatic hydrolysis Nutrition. Foods and food supply TX341-641 |
spellingShingle |
Hydrolysates Immunomodulatory peptide Purification Amino acid sequence Enzymatic hydrolysis Nutrition. Foods and food supply TX341-641 Hu Hou Yan Fan Shikai Wang Leilei Si Bafang Li Immunomodulatory activity of Alaska pollock hydrolysates obtained by glutamic acid biosensor – Artificial neural network and the identification of its active central fragment |
topic_facet |
Hydrolysates Immunomodulatory peptide Purification Amino acid sequence Enzymatic hydrolysis Nutrition. Foods and food supply TX341-641 |
description |
The glutamic acid biosensor and artificial neural network (GLU-ANN) was employed to monitor the hydrolysis of Alaska pollock protein for production of immunomodulatory peptides. The relative error of GLU-ANN was in the range of 0.23% to 2.81%. The target pollock hydrolysates (PFH) were prepared using GLU-ANN, which significantly enhanced humoral, cellular, and non-specific immunity in immunosuppressed mice, induced by hydrogenated cortisone (p < 0.05). In addition, treatment with PFH significantly increased the production of interleukin (IL)-2, IL-4, and IL-6 in immunosuppressed mice (p < 0.05), while interferon-gamma (IFN-γ) showed no significant change. The active central fragment that exhibited the highest lymphocyte proliferation rates was further purified using ion exchange chromatography, gel filtration chromatography, and reversed-phase high performance liquid chromatography (RP-HPLC). The amino acid sequence of this fragment was as follows: Pro-Thr-Gly-Ala-Asp-Tyr (PTGADY). Therefore, PFH shows promise as a potent immunomodulator with potential applications in functional food. |
format |
Article in Journal/Newspaper |
author |
Hu Hou Yan Fan Shikai Wang Leilei Si Bafang Li |
author_facet |
Hu Hou Yan Fan Shikai Wang Leilei Si Bafang Li |
author_sort |
Hu Hou |
title |
Immunomodulatory activity of Alaska pollock hydrolysates obtained by glutamic acid biosensor – Artificial neural network and the identification of its active central fragment |
title_short |
Immunomodulatory activity of Alaska pollock hydrolysates obtained by glutamic acid biosensor – Artificial neural network and the identification of its active central fragment |
title_full |
Immunomodulatory activity of Alaska pollock hydrolysates obtained by glutamic acid biosensor – Artificial neural network and the identification of its active central fragment |
title_fullStr |
Immunomodulatory activity of Alaska pollock hydrolysates obtained by glutamic acid biosensor – Artificial neural network and the identification of its active central fragment |
title_full_unstemmed |
Immunomodulatory activity of Alaska pollock hydrolysates obtained by glutamic acid biosensor – Artificial neural network and the identification of its active central fragment |
title_sort |
immunomodulatory activity of alaska pollock hydrolysates obtained by glutamic acid biosensor – artificial neural network and the identification of its active central fragment |
publisher |
Elsevier |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.jff.2016.03.033 https://doaj.org/article/cf50e6741c904c9eb6763e20590c3bb9 |
genre |
alaska pollock Alaska |
genre_facet |
alaska pollock Alaska |
op_source |
Journal of Functional Foods, Vol 24, Iss , Pp 37-47 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S1756464616300561 https://doaj.org/toc/1756-4646 1756-4646 doi:10.1016/j.jff.2016.03.033 https://doaj.org/article/cf50e6741c904c9eb6763e20590c3bb9 |
op_doi |
https://doi.org/10.1016/j.jff.2016.03.033 |
container_title |
Journal of Functional Foods |
container_volume |
24 |
container_start_page |
37 |
op_container_end_page |
47 |
_version_ |
1766173247091507200 |