An advanced enrichment method for rare somatic retroelement insertions sequencing
Abstract Background There is increasing evidence that the transpositional activity of retroelements (REs) is not limited to germ line cells, but often occurs in tumor and normal somatic cells. Somatic transpositions were found in several human tissues and are especially typical for the brain. Severa...
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ftdoajarticles:oai:doaj.org/article:cf35768c383146e68c0de6bd418e038c 2023-05-15T16:59:16+02:00 An advanced enrichment method for rare somatic retroelement insertions sequencing Alexander Y. Komkov Anastasia A. Minervina Gaiaz A. Nugmanov Mariia V. Saliutina Konstantin V. Khodosevich Yuri B. Lebedev Ilgar Z. Mamedov 2018-10-01T00:00:00Z https://doi.org/10.1186/s13100-018-0136-1 https://doaj.org/article/cf35768c383146e68c0de6bd418e038c EN eng BMC http://link.springer.com/article/10.1186/s13100-018-0136-1 https://doaj.org/toc/1759-8753 doi:10.1186/s13100-018-0136-1 1759-8753 https://doaj.org/article/cf35768c383146e68c0de6bd418e038c Mobile DNA, Vol 9, Iss 1, Pp 1-11 (2018) Somatic retroelement insertions Genomic normalization Kamchatka Crab duplex-specific nuclease Genetics QH426-470 article 2018 ftdoajarticles https://doi.org/10.1186/s13100-018-0136-1 2022-12-31T15:47:17Z Abstract Background There is increasing evidence that the transpositional activity of retroelements (REs) is not limited to germ line cells, but often occurs in tumor and normal somatic cells. Somatic transpositions were found in several human tissues and are especially typical for the brain. Several computational and experimental approaches for detection of somatic retroelement insertions was developed in the past few years. These approaches were successfully applied to detect somatic insertions in clonally expanded tumor cells. At the same time, identification of somatic insertions presented in small proportion of cells, such as neurons, remains a considerable challenge. Results In this study, we developed a normalization procedure for library enrichment by DNA sequences corresponding to rare somatic RE insertions. Two rounds of normalization increased the number of fragments adjacent to somatic REs in the sequenced sample by more than 26-fold, and the number of identified somatic REs was increased by 8-fold. Conclusions The developed technique can be used in combination with vast majority of modern RE identification approaches and can dramatically increase their capacity to detect rare somatic RE insertions in different types of cells. Article in Journal/Newspaper Kamchatka Kamchatka crab Directory of Open Access Journals: DOAJ Articles Mobile DNA 9 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Somatic retroelement insertions Genomic normalization Kamchatka Crab duplex-specific nuclease Genetics QH426-470 |
spellingShingle |
Somatic retroelement insertions Genomic normalization Kamchatka Crab duplex-specific nuclease Genetics QH426-470 Alexander Y. Komkov Anastasia A. Minervina Gaiaz A. Nugmanov Mariia V. Saliutina Konstantin V. Khodosevich Yuri B. Lebedev Ilgar Z. Mamedov An advanced enrichment method for rare somatic retroelement insertions sequencing |
topic_facet |
Somatic retroelement insertions Genomic normalization Kamchatka Crab duplex-specific nuclease Genetics QH426-470 |
description |
Abstract Background There is increasing evidence that the transpositional activity of retroelements (REs) is not limited to germ line cells, but often occurs in tumor and normal somatic cells. Somatic transpositions were found in several human tissues and are especially typical for the brain. Several computational and experimental approaches for detection of somatic retroelement insertions was developed in the past few years. These approaches were successfully applied to detect somatic insertions in clonally expanded tumor cells. At the same time, identification of somatic insertions presented in small proportion of cells, such as neurons, remains a considerable challenge. Results In this study, we developed a normalization procedure for library enrichment by DNA sequences corresponding to rare somatic RE insertions. Two rounds of normalization increased the number of fragments adjacent to somatic REs in the sequenced sample by more than 26-fold, and the number of identified somatic REs was increased by 8-fold. Conclusions The developed technique can be used in combination with vast majority of modern RE identification approaches and can dramatically increase their capacity to detect rare somatic RE insertions in different types of cells. |
format |
Article in Journal/Newspaper |
author |
Alexander Y. Komkov Anastasia A. Minervina Gaiaz A. Nugmanov Mariia V. Saliutina Konstantin V. Khodosevich Yuri B. Lebedev Ilgar Z. Mamedov |
author_facet |
Alexander Y. Komkov Anastasia A. Minervina Gaiaz A. Nugmanov Mariia V. Saliutina Konstantin V. Khodosevich Yuri B. Lebedev Ilgar Z. Mamedov |
author_sort |
Alexander Y. Komkov |
title |
An advanced enrichment method for rare somatic retroelement insertions sequencing |
title_short |
An advanced enrichment method for rare somatic retroelement insertions sequencing |
title_full |
An advanced enrichment method for rare somatic retroelement insertions sequencing |
title_fullStr |
An advanced enrichment method for rare somatic retroelement insertions sequencing |
title_full_unstemmed |
An advanced enrichment method for rare somatic retroelement insertions sequencing |
title_sort |
advanced enrichment method for rare somatic retroelement insertions sequencing |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s13100-018-0136-1 https://doaj.org/article/cf35768c383146e68c0de6bd418e038c |
genre |
Kamchatka Kamchatka crab |
genre_facet |
Kamchatka Kamchatka crab |
op_source |
Mobile DNA, Vol 9, Iss 1, Pp 1-11 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s13100-018-0136-1 https://doaj.org/toc/1759-8753 doi:10.1186/s13100-018-0136-1 1759-8753 https://doaj.org/article/cf35768c383146e68c0de6bd418e038c |
op_doi |
https://doi.org/10.1186/s13100-018-0136-1 |
container_title |
Mobile DNA |
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9 |
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1 |
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1766051500212092928 |