Borassus flabellifer L. crude male flower extracts alleviate cisplatin-induced oxidative stress in rat kidney cells
Objective: To investigate the effects of Borassus flabellifer L. extracts on antioxidant activity, maintenance of cellular redox, and mitochondrial function in cisplatin-induced kidney injury. Methods: The extracts of Borassus flabellifer were obtained from crude male flowers using ethyl acetate and...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2021
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Online Access: | https://doi.org/10.4103/2221-1691.303607 https://doaj.org/article/cf2c0f6b8b72440b8a7f6bc903f4e17d |
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ftdoajarticles:oai:doaj.org/article:cf2c0f6b8b72440b8a7f6bc903f4e17d 2023-05-15T15:09:16+02:00 Borassus flabellifer L. crude male flower extracts alleviate cisplatin-induced oxidative stress in rat kidney cells Ornanong Tusskorn Kanoktip Pansuksan Kwanchayanawish Machana 2021-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.303607 https://doaj.org/article/cf2c0f6b8b72440b8a7f6bc903f4e17d EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=2;spage=81;epage=88;aulast=Tusskorn https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.303607 https://doaj.org/article/cf2c0f6b8b72440b8a7f6bc903f4e17d Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 2, Pp 81-88 (2021) borassus flabellifer l. cisplatin oxidative stress mitochondrial dysfunction Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.4103/2221-1691.303607 2022-12-30T19:54:42Z Objective: To investigate the effects of Borassus flabellifer L. extracts on antioxidant activity, maintenance of cellular redox, and mitochondrial function in cisplatin-induced kidney injury. Methods: The extracts of Borassus flabellifer were obtained from crude male flowers using ethyl acetate and methanol. The antioxidant potential was evaluated by 2,2-azino-bis-(3-ethylbenzothiazoline- 6-sulfonic acid), 2,2-diphenyl-1-picrylhydrazyl, and ferric reducing antioxidant power, and total phenolic content was also determined. Cytoprotective activity of ethyl acetate and methanolic extracts was assessed after kidney cells were treated with cisplatin. Oxidative stress was determined by glutathione (GSH) assay, and formation of reactive oxygen species (ROS) and changes in mitochondrial transmembrane potential (ΔΨm) using 2’,7’-dichlorofluorescin diacetate and JC-10 assays, respectively. Results: Borassus flabellifer methanolic extract exhibited greater antioxidant activity than the ethyl acetate extract. Cytoprotective effect was demonstrated in both extracts, particularly in the ethyl acetate extract. The extracts showed protection against the cytotoxic effect of cisplatin by prevention of the increased GSSG and declined GSH/GSSG ratio. Both extracts also prevented the increase in ROS formation, and loss of ΔΨm. Conclusions: Both Borassus flabellifer extracts show antioxidant activity and cytoprotective effect against cisplatin-induced cytotoxicity of NRK-52E cells by preventing oxidative stress and maintenance of GSH redox status. Borassus flabellifer extracts may possess beneficial effects on the prevention of oxidative stress- induced cell injury. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 11 2 81 |
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borassus flabellifer l. cisplatin oxidative stress mitochondrial dysfunction Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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borassus flabellifer l. cisplatin oxidative stress mitochondrial dysfunction Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Ornanong Tusskorn Kanoktip Pansuksan Kwanchayanawish Machana Borassus flabellifer L. crude male flower extracts alleviate cisplatin-induced oxidative stress in rat kidney cells |
topic_facet |
borassus flabellifer l. cisplatin oxidative stress mitochondrial dysfunction Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the effects of Borassus flabellifer L. extracts on antioxidant activity, maintenance of cellular redox, and mitochondrial function in cisplatin-induced kidney injury. Methods: The extracts of Borassus flabellifer were obtained from crude male flowers using ethyl acetate and methanol. The antioxidant potential was evaluated by 2,2-azino-bis-(3-ethylbenzothiazoline- 6-sulfonic acid), 2,2-diphenyl-1-picrylhydrazyl, and ferric reducing antioxidant power, and total phenolic content was also determined. Cytoprotective activity of ethyl acetate and methanolic extracts was assessed after kidney cells were treated with cisplatin. Oxidative stress was determined by glutathione (GSH) assay, and formation of reactive oxygen species (ROS) and changes in mitochondrial transmembrane potential (ΔΨm) using 2’,7’-dichlorofluorescin diacetate and JC-10 assays, respectively. Results: Borassus flabellifer methanolic extract exhibited greater antioxidant activity than the ethyl acetate extract. Cytoprotective effect was demonstrated in both extracts, particularly in the ethyl acetate extract. The extracts showed protection against the cytotoxic effect of cisplatin by prevention of the increased GSSG and declined GSH/GSSG ratio. Both extracts also prevented the increase in ROS formation, and loss of ΔΨm. Conclusions: Both Borassus flabellifer extracts show antioxidant activity and cytoprotective effect against cisplatin-induced cytotoxicity of NRK-52E cells by preventing oxidative stress and maintenance of GSH redox status. Borassus flabellifer extracts may possess beneficial effects on the prevention of oxidative stress- induced cell injury. |
format |
Article in Journal/Newspaper |
author |
Ornanong Tusskorn Kanoktip Pansuksan Kwanchayanawish Machana |
author_facet |
Ornanong Tusskorn Kanoktip Pansuksan Kwanchayanawish Machana |
author_sort |
Ornanong Tusskorn |
title |
Borassus flabellifer L. crude male flower extracts alleviate cisplatin-induced oxidative stress in rat kidney cells |
title_short |
Borassus flabellifer L. crude male flower extracts alleviate cisplatin-induced oxidative stress in rat kidney cells |
title_full |
Borassus flabellifer L. crude male flower extracts alleviate cisplatin-induced oxidative stress in rat kidney cells |
title_fullStr |
Borassus flabellifer L. crude male flower extracts alleviate cisplatin-induced oxidative stress in rat kidney cells |
title_full_unstemmed |
Borassus flabellifer L. crude male flower extracts alleviate cisplatin-induced oxidative stress in rat kidney cells |
title_sort |
borassus flabellifer l. crude male flower extracts alleviate cisplatin-induced oxidative stress in rat kidney cells |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2021 |
url |
https://doi.org/10.4103/2221-1691.303607 https://doaj.org/article/cf2c0f6b8b72440b8a7f6bc903f4e17d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 2, Pp 81-88 (2021) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=2;spage=81;epage=88;aulast=Tusskorn https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.303607 https://doaj.org/article/cf2c0f6b8b72440b8a7f6bc903f4e17d |
op_doi |
https://doi.org/10.4103/2221-1691.303607 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
11 |
container_issue |
2 |
container_start_page |
81 |
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1766340487268007936 |