Sampling is decisive to determination of Leishmania (Viannia) species.
Background Accuracy of molecular tools for the identification of parasites that cause human cutaneous leishmaniasis (CL) could largely depend on the sampling method. Non-invasive or less-invasive sampling methods such as filter paper imprints and cotton swabs are preferred over punch biopsies and la...
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ftdoajarticles:oai:doaj.org/article:cefe22eb4c074dac98a9808294581f80 2024-09-09T19:27:45+00:00 Sampling is decisive to determination of Leishmania (Viannia) species. Maxy B De Los Santos Steev Loyola Erika S Perez-Velez Rocio Del Pilar Santos Ivonne Melissa Ramírez Hugo O Valdivia 2024-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0012113 https://doaj.org/article/cefe22eb4c074dac98a9808294581f80 EN eng Public Library of Science (PLoS) https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0012113&type=printable https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0012113 https://doaj.org/article/cefe22eb4c074dac98a9808294581f80 PLoS Neglected Tropical Diseases, Vol 18, Iss 4, p e0012113 (2024) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2024 ftdoajarticles https://doi.org/10.1371/journal.pntd.0012113 2024-08-05T17:49:27Z Background Accuracy of molecular tools for the identification of parasites that cause human cutaneous leishmaniasis (CL) could largely depend on the sampling method. Non-invasive or less-invasive sampling methods such as filter paper imprints and cotton swabs are preferred over punch biopsies and lancet scrapings for detection methods of Leishmania based on polymerase chain reaction (PCR) because they are painless, simple, and inexpensive, and of benefit to military and civilian patients to ensure timely treatment. However, different types of samples can generate false negatives and there is a clear need to demonstrate which sample is more proper for molecular assays. Methodology Here, we compared the sensitivity of molecular identification of different Leishmania (Viannia) species from Peru, using three types of sampling: punch biopsy, filter paper imprint and lancet scraping. Different composite reference standards and latent class models allowed to evaluate the accuracy of the molecular tools. Additionally, a quantitative PCR assessed variations in the results and parasite load in each type of sample. Principal findings Different composite reference standards and latent class models determined higher sensitivity when lancet scrapings were used for sampling in the identification and determination of Leishmania (Viannia) species through PCR-based assays. This was consistent for genus identification through kinetoplastid DNA-PCR and for the determination of species using FRET probes-based Nested Real-Time PCR. Lack of species identification in some samples correlated with the low intensity of the PCR electrophoretic band, which reflects the low parasite load in samples. Conclusions The type of clinical sample can directly influence the detection and identification of Leishmania (Viannia) species. Here, we demonstrated that lancet scraping samples consistently allowed the identification of more leishmaniasis cases compared to filter paper imprints or biopsies. This procedure is inexpensive, painless, and easy to ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 18 4 e0012113 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Maxy B De Los Santos Steev Loyola Erika S Perez-Velez Rocio Del Pilar Santos Ivonne Melissa Ramírez Hugo O Valdivia Sampling is decisive to determination of Leishmania (Viannia) species. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Accuracy of molecular tools for the identification of parasites that cause human cutaneous leishmaniasis (CL) could largely depend on the sampling method. Non-invasive or less-invasive sampling methods such as filter paper imprints and cotton swabs are preferred over punch biopsies and lancet scrapings for detection methods of Leishmania based on polymerase chain reaction (PCR) because they are painless, simple, and inexpensive, and of benefit to military and civilian patients to ensure timely treatment. However, different types of samples can generate false negatives and there is a clear need to demonstrate which sample is more proper for molecular assays. Methodology Here, we compared the sensitivity of molecular identification of different Leishmania (Viannia) species from Peru, using three types of sampling: punch biopsy, filter paper imprint and lancet scraping. Different composite reference standards and latent class models allowed to evaluate the accuracy of the molecular tools. Additionally, a quantitative PCR assessed variations in the results and parasite load in each type of sample. Principal findings Different composite reference standards and latent class models determined higher sensitivity when lancet scrapings were used for sampling in the identification and determination of Leishmania (Viannia) species through PCR-based assays. This was consistent for genus identification through kinetoplastid DNA-PCR and for the determination of species using FRET probes-based Nested Real-Time PCR. Lack of species identification in some samples correlated with the low intensity of the PCR electrophoretic band, which reflects the low parasite load in samples. Conclusions The type of clinical sample can directly influence the detection and identification of Leishmania (Viannia) species. Here, we demonstrated that lancet scraping samples consistently allowed the identification of more leishmaniasis cases compared to filter paper imprints or biopsies. This procedure is inexpensive, painless, and easy to ... |
format |
Article in Journal/Newspaper |
author |
Maxy B De Los Santos Steev Loyola Erika S Perez-Velez Rocio Del Pilar Santos Ivonne Melissa Ramírez Hugo O Valdivia |
author_facet |
Maxy B De Los Santos Steev Loyola Erika S Perez-Velez Rocio Del Pilar Santos Ivonne Melissa Ramírez Hugo O Valdivia |
author_sort |
Maxy B De Los Santos |
title |
Sampling is decisive to determination of Leishmania (Viannia) species. |
title_short |
Sampling is decisive to determination of Leishmania (Viannia) species. |
title_full |
Sampling is decisive to determination of Leishmania (Viannia) species. |
title_fullStr |
Sampling is decisive to determination of Leishmania (Viannia) species. |
title_full_unstemmed |
Sampling is decisive to determination of Leishmania (Viannia) species. |
title_sort |
sampling is decisive to determination of leishmania (viannia) species. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2024 |
url |
https://doi.org/10.1371/journal.pntd.0012113 https://doaj.org/article/cefe22eb4c074dac98a9808294581f80 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 18, Iss 4, p e0012113 (2024) |
op_relation |
https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0012113&type=printable https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0012113 https://doaj.org/article/cefe22eb4c074dac98a9808294581f80 |
op_doi |
https://doi.org/10.1371/journal.pntd.0012113 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
18 |
container_issue |
4 |
container_start_page |
e0012113 |
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1809897128557805568 |