Effects of Short-Term Fasting on mRNA Expression of Ghrelin and the Peptide Transporters PepT1 and 2 in Atlantic Salmon (Salmo salar)
Food intake is a vital process that supplies necessary energy and essential nutrients to the body. Information regarding luminal composition in the gastrointestinal tract (GIT) collected through mechanical and nutrient sensing mechanisms are generally conveyed, in both mammals and fish, to the hypot...
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ftdoajarticles:oai:doaj.org/article:ceba5db3eac443f09b88cd736557c79d 2023-05-15T15:32:01+02:00 Effects of Short-Term Fasting on mRNA Expression of Ghrelin and the Peptide Transporters PepT1 and 2 in Atlantic Salmon (Salmo salar) Gianmarco Del Vecchio Floriana Lai Ana S. Gomes Tiziano Verri Tharmini Kalananthan Amilcare Barca Sigurd Handeland Ivar Rønnestad 2021-06-01T00:00:00Z https://doi.org/10.3389/fphys.2021.666670 https://doaj.org/article/ceba5db3eac443f09b88cd736557c79d EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fphys.2021.666670/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2021.666670 https://doaj.org/article/ceba5db3eac443f09b88cd736557c79d Frontiers in Physiology, Vol 12 (2021) ghrelin peptide transporters slc15 fasting digestive tract fish Physiology QP1-981 article 2021 ftdoajarticles https://doi.org/10.3389/fphys.2021.666670 2022-12-31T14:48:00Z Food intake is a vital process that supplies necessary energy and essential nutrients to the body. Information regarding luminal composition in the gastrointestinal tract (GIT) collected through mechanical and nutrient sensing mechanisms are generally conveyed, in both mammals and fish, to the hypothalamic neurocircuits. In this context, ghrelin, the only known hormone with an orexigenic action, and the intestinal peptide transporters 1 and 2, involved in absorption of dietary di- and tripeptides, exert important and also integrated roles for the nutrient uptake. Together, both are potentially involved in signaling pathways that control food intake originating from different segments of the GIT. However, little is known about the role of different paralogs and their response to fasting. Therefore, after 3 weeks of acclimatization, 12 Atlantic salmon (Salmo salar) post-smolt were fasted for 4 days to explore the gastrointestinal response in comparison with fed control (n = 12). The analysis covered morphometric (weight, length, condition factor, and wet content/weight fish %), molecular (gene expression variations), and correlation analyses. Such short-term fasting is a common and recommended practice used prior to any handling in commercial culture of the species. There were no statistical differences in length and weight but a significant lower condition factor in the fasted group. Transcriptional analysis along the gastrointestinal segments revealed a tendency of downregulation for both paralogous genes slc15a1a and slc15a1b and with significant lowered levels in the pyloric ceca for slc15a1a and in the pyloric ceca and midgut for slc15a1b. No differences were found for slc15a2a and slc15a2b (except a higher expression of the fasted group in the anterior midgut), supporting different roles for slc15 paralogs. This represents the first report on the effects of fasting on slc15a2 expressed in GIT in teleosts. Transcriptional analysis of ghrelin splicing variants (ghrl-1 and ghrl-2) showed no difference between ... Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Frontiers in Physiology 12 |
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ghrelin peptide transporters slc15 fasting digestive tract fish Physiology QP1-981 |
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ghrelin peptide transporters slc15 fasting digestive tract fish Physiology QP1-981 Gianmarco Del Vecchio Floriana Lai Ana S. Gomes Tiziano Verri Tharmini Kalananthan Amilcare Barca Sigurd Handeland Ivar Rønnestad Effects of Short-Term Fasting on mRNA Expression of Ghrelin and the Peptide Transporters PepT1 and 2 in Atlantic Salmon (Salmo salar) |
topic_facet |
ghrelin peptide transporters slc15 fasting digestive tract fish Physiology QP1-981 |
description |
Food intake is a vital process that supplies necessary energy and essential nutrients to the body. Information regarding luminal composition in the gastrointestinal tract (GIT) collected through mechanical and nutrient sensing mechanisms are generally conveyed, in both mammals and fish, to the hypothalamic neurocircuits. In this context, ghrelin, the only known hormone with an orexigenic action, and the intestinal peptide transporters 1 and 2, involved in absorption of dietary di- and tripeptides, exert important and also integrated roles for the nutrient uptake. Together, both are potentially involved in signaling pathways that control food intake originating from different segments of the GIT. However, little is known about the role of different paralogs and their response to fasting. Therefore, after 3 weeks of acclimatization, 12 Atlantic salmon (Salmo salar) post-smolt were fasted for 4 days to explore the gastrointestinal response in comparison with fed control (n = 12). The analysis covered morphometric (weight, length, condition factor, and wet content/weight fish %), molecular (gene expression variations), and correlation analyses. Such short-term fasting is a common and recommended practice used prior to any handling in commercial culture of the species. There were no statistical differences in length and weight but a significant lower condition factor in the fasted group. Transcriptional analysis along the gastrointestinal segments revealed a tendency of downregulation for both paralogous genes slc15a1a and slc15a1b and with significant lowered levels in the pyloric ceca for slc15a1a and in the pyloric ceca and midgut for slc15a1b. No differences were found for slc15a2a and slc15a2b (except a higher expression of the fasted group in the anterior midgut), supporting different roles for slc15 paralogs. This represents the first report on the effects of fasting on slc15a2 expressed in GIT in teleosts. Transcriptional analysis of ghrelin splicing variants (ghrl-1 and ghrl-2) showed no difference between ... |
format |
Article in Journal/Newspaper |
author |
Gianmarco Del Vecchio Floriana Lai Ana S. Gomes Tiziano Verri Tharmini Kalananthan Amilcare Barca Sigurd Handeland Ivar Rønnestad |
author_facet |
Gianmarco Del Vecchio Floriana Lai Ana S. Gomes Tiziano Verri Tharmini Kalananthan Amilcare Barca Sigurd Handeland Ivar Rønnestad |
author_sort |
Gianmarco Del Vecchio |
title |
Effects of Short-Term Fasting on mRNA Expression of Ghrelin and the Peptide Transporters PepT1 and 2 in Atlantic Salmon (Salmo salar) |
title_short |
Effects of Short-Term Fasting on mRNA Expression of Ghrelin and the Peptide Transporters PepT1 and 2 in Atlantic Salmon (Salmo salar) |
title_full |
Effects of Short-Term Fasting on mRNA Expression of Ghrelin and the Peptide Transporters PepT1 and 2 in Atlantic Salmon (Salmo salar) |
title_fullStr |
Effects of Short-Term Fasting on mRNA Expression of Ghrelin and the Peptide Transporters PepT1 and 2 in Atlantic Salmon (Salmo salar) |
title_full_unstemmed |
Effects of Short-Term Fasting on mRNA Expression of Ghrelin and the Peptide Transporters PepT1 and 2 in Atlantic Salmon (Salmo salar) |
title_sort |
effects of short-term fasting on mrna expression of ghrelin and the peptide transporters pept1 and 2 in atlantic salmon (salmo salar) |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fphys.2021.666670 https://doaj.org/article/ceba5db3eac443f09b88cd736557c79d |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Frontiers in Physiology, Vol 12 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fphys.2021.666670/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2021.666670 https://doaj.org/article/ceba5db3eac443f09b88cd736557c79d |
op_doi |
https://doi.org/10.3389/fphys.2021.666670 |
container_title |
Frontiers in Physiology |
container_volume |
12 |
_version_ |
1766362519127982080 |