Free radical scavenging and anti-proliferative activities of avocado (Persea americana Mill.) seed extract
Objective: To investigate the chemical components and biological activities of avocado seed extract and fractions in order to determine the nutritional and pharmaceutical values of avocado seed. Methods: Various organic solvents were applied for extraction and fractionation of avocado seed. 1,1-diph...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
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Online Access: | https://doi.org/10.4103/2221-1691.254602 https://doaj.org/article/cea97c7c1e464ce9b3b830f17b55f659 |
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ftdoajarticles:oai:doaj.org/article:cea97c7c1e464ce9b3b830f17b55f659 2023-05-15T15:11:41+02:00 Free radical scavenging and anti-proliferative activities of avocado (Persea americana Mill.) seed extract Thanh Sang Vo Phuong Uyen Le Dai Hung Ngo 2019-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.254602 https://doaj.org/article/cea97c7c1e464ce9b3b830f17b55f659 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=3;spage=91;epage=97;aulast=Vo https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.254602 https://doaj.org/article/cea97c7c1e464ce9b3b830f17b55f659 Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 3, Pp 91-97 (2019) avocado seed by-product dpph nitric oxide cancer cells Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.4103/2221-1691.254602 2022-12-31T03:12:13Z Objective: To investigate the chemical components and biological activities of avocado seed extract and fractions in order to determine the nutritional and pharmaceutical values of avocado seed. Methods: Various organic solvents were applied for extraction and fractionation of avocado seed. 1,1-diphenyl-2-picryl-hydrazyl, 2,2-azinobis-3-ethyl benzothiazoline-6-sulfonic acid, and DNA oxidation assays were applied for investigation of free radical scavenging activity. Nitric oxide production was measured by Griess reaction assay. Moreover, MTT assay was used to measure cancer cell growth inhibition. Results: The result indicated that the avocado seed contains (7.14 ± 0.40) g lipid/100 g, (1.67 ± 0.03) g protein/100 g, (54.0 ± 1.2) g carbohydrate/100 g, and (62.0 ± 2.3) mg gallic acid equivalent/g dried weight extract. Moreover, dichloromethane and ethyl acetate were revealed to be the highest free radical scavenging fractions with IC50 values of (48.0 ± 3.4) μg/mL (1,1-diphenyl-2-picryl-hydrazyl assay) and (22.0 ± 1.8) μg/mL (2,2-azinobis-3-ethyl benzothiazoline-6-sulfonic acid assay), respectively. Additionally, the avocado seed extract and fractions were able to protect against H2O2-induced DNA damage at the concentration of 100 μg/mL. On the other hand, the ethanol extract was effective in reducing nitric oxide production from lipopolysaccharide-stimulated RAW 264.7 macrophage cells without cytotoxic effect. Notably, the avocado seed significantly inhibited the proliferation of human lung A549 and human gastric BGC823 cancer cells at the concentration of 200 μg/mL, especially hexane (81 ± 3)% and dichloromethane (75 ± 2)% fractions. Conclusions: The results of the present study supported the avocado seed as potential by-product source for further development of health beneficial products. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 9 3 91 |
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topic |
avocado seed by-product dpph nitric oxide cancer cells Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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avocado seed by-product dpph nitric oxide cancer cells Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Thanh Sang Vo Phuong Uyen Le Dai Hung Ngo Free radical scavenging and anti-proliferative activities of avocado (Persea americana Mill.) seed extract |
topic_facet |
avocado seed by-product dpph nitric oxide cancer cells Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the chemical components and biological activities of avocado seed extract and fractions in order to determine the nutritional and pharmaceutical values of avocado seed. Methods: Various organic solvents were applied for extraction and fractionation of avocado seed. 1,1-diphenyl-2-picryl-hydrazyl, 2,2-azinobis-3-ethyl benzothiazoline-6-sulfonic acid, and DNA oxidation assays were applied for investigation of free radical scavenging activity. Nitric oxide production was measured by Griess reaction assay. Moreover, MTT assay was used to measure cancer cell growth inhibition. Results: The result indicated that the avocado seed contains (7.14 ± 0.40) g lipid/100 g, (1.67 ± 0.03) g protein/100 g, (54.0 ± 1.2) g carbohydrate/100 g, and (62.0 ± 2.3) mg gallic acid equivalent/g dried weight extract. Moreover, dichloromethane and ethyl acetate were revealed to be the highest free radical scavenging fractions with IC50 values of (48.0 ± 3.4) μg/mL (1,1-diphenyl-2-picryl-hydrazyl assay) and (22.0 ± 1.8) μg/mL (2,2-azinobis-3-ethyl benzothiazoline-6-sulfonic acid assay), respectively. Additionally, the avocado seed extract and fractions were able to protect against H2O2-induced DNA damage at the concentration of 100 μg/mL. On the other hand, the ethanol extract was effective in reducing nitric oxide production from lipopolysaccharide-stimulated RAW 264.7 macrophage cells without cytotoxic effect. Notably, the avocado seed significantly inhibited the proliferation of human lung A549 and human gastric BGC823 cancer cells at the concentration of 200 μg/mL, especially hexane (81 ± 3)% and dichloromethane (75 ± 2)% fractions. Conclusions: The results of the present study supported the avocado seed as potential by-product source for further development of health beneficial products. |
format |
Article in Journal/Newspaper |
author |
Thanh Sang Vo Phuong Uyen Le Dai Hung Ngo |
author_facet |
Thanh Sang Vo Phuong Uyen Le Dai Hung Ngo |
author_sort |
Thanh Sang Vo |
title |
Free radical scavenging and anti-proliferative activities of avocado (Persea americana Mill.) seed extract |
title_short |
Free radical scavenging and anti-proliferative activities of avocado (Persea americana Mill.) seed extract |
title_full |
Free radical scavenging and anti-proliferative activities of avocado (Persea americana Mill.) seed extract |
title_fullStr |
Free radical scavenging and anti-proliferative activities of avocado (Persea americana Mill.) seed extract |
title_full_unstemmed |
Free radical scavenging and anti-proliferative activities of avocado (Persea americana Mill.) seed extract |
title_sort |
free radical scavenging and anti-proliferative activities of avocado (persea americana mill.) seed extract |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/2221-1691.254602 https://doaj.org/article/cea97c7c1e464ce9b3b830f17b55f659 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 3, Pp 91-97 (2019) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=3;spage=91;epage=97;aulast=Vo https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.254602 https://doaj.org/article/cea97c7c1e464ce9b3b830f17b55f659 |
op_doi |
https://doi.org/10.4103/2221-1691.254602 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
9 |
container_issue |
3 |
container_start_page |
91 |
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1766342504803729408 |