Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera
Leishmania braziliensis braziliensis(MHOM/BR/75/M2903) was grown in Schneider's Drosophila medium. In one set of experiments promastigotes were already adapted to the medium by means of serial passages whereas in the second cells were grown in a biphasic medium and transfered to the liquid. Gro...
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Universidade de São Paulo (USP)
1988
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Online Access: | https://doi.org/10.1590/S0036-46651988000200001 https://doaj.org/article/ce2a367ec94c44ce86b2774b4a79a16a |
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ftdoajarticles:oai:doaj.org/article:ce2a367ec94c44ce86b2774b4a79a16a 2024-09-09T19:25:10+00:00 Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera Beatriz J. Celeste M. Carolina S. Guimarães 1988-04-01T00:00:00Z https://doi.org/10.1590/S0036-46651988000200001 https://doaj.org/article/ce2a367ec94c44ce86b2774b4a79a16a EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651988000200001&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46651988000200001 https://doaj.org/article/ce2a367ec94c44ce86b2774b4a79a16a Revista do Instituto de Medicina Tropical de São Paulo, Vol 30, Iss 2, Pp 63-67 (1988) Leishmania braziliensis braziliensis Culture Antigen Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1988 ftdoajarticles https://doi.org/10.1590/S0036-46651988000200001 2024-08-05T17:49:30Z Leishmania braziliensis braziliensis(MHOM/BR/75/M2903) was grown in Schneider's Drosophila medium. In one set of experiments promastigotes were already adapted to the medium by means of serial passages whereas in the second cells were grown in a biphasic medium and transfered to the liquid. Growth was more abundant for culture medium adapted cells; degenerate cells in small numbers as well as dead ones were present from day 5 for promastigotes adapted to liquid medium and from day 3 for newly adapted cells. Synthesis of surface antigens differed according to length of cell culture as assessed by the titer of five mucocutaneous leishmaniasis sera on subsequent days. Five days of culture for cells already adapted to the culture medium and 3 days for newly adapted ones were judged to be the best for the preparation of immunofluorescence antigens. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 30 2 63 67 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
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language |
English |
topic |
Leishmania braziliensis braziliensis Culture Antigen Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Leishmania braziliensis braziliensis Culture Antigen Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Beatriz J. Celeste M. Carolina S. Guimarães Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera |
topic_facet |
Leishmania braziliensis braziliensis Culture Antigen Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Leishmania braziliensis braziliensis(MHOM/BR/75/M2903) was grown in Schneider's Drosophila medium. In one set of experiments promastigotes were already adapted to the medium by means of serial passages whereas in the second cells were grown in a biphasic medium and transfered to the liquid. Growth was more abundant for culture medium adapted cells; degenerate cells in small numbers as well as dead ones were present from day 5 for promastigotes adapted to liquid medium and from day 3 for newly adapted cells. Synthesis of surface antigens differed according to length of cell culture as assessed by the titer of five mucocutaneous leishmaniasis sera on subsequent days. Five days of culture for cells already adapted to the culture medium and 3 days for newly adapted ones were judged to be the best for the preparation of immunofluorescence antigens. |
format |
Article in Journal/Newspaper |
author |
Beatriz J. Celeste M. Carolina S. Guimarães |
author_facet |
Beatriz J. Celeste M. Carolina S. Guimarães |
author_sort |
Beatriz J. Celeste |
title |
Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera |
title_short |
Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera |
title_full |
Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera |
title_fullStr |
Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera |
title_full_unstemmed |
Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera |
title_sort |
growth curves of leishmania braziliensis braziliensis promastigotes and surface antigen expression before and after adaptation to schneider's drosophila medium as assessed by anti-leishmania human sera |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1988 |
url |
https://doi.org/10.1590/S0036-46651988000200001 https://doaj.org/article/ce2a367ec94c44ce86b2774b4a79a16a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 30, Iss 2, Pp 63-67 (1988) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651988000200001&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46651988000200001 https://doaj.org/article/ce2a367ec94c44ce86b2774b4a79a16a |
op_doi |
https://doi.org/10.1590/S0036-46651988000200001 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
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30 |
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2 |
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63 |
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67 |
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