Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model Caracterização molecular de cepas mexicanas de Trypanosoma cruzi e seu comportamento no modelo experimental do camundongo
INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on th...
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ftdoajarticles:oai:doaj.org/article:ce0aa25602a54efa908a2473e89a923d 2023-05-15T15:17:53+02:00 Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model Caracterização molecular de cepas mexicanas de Trypanosoma cruzi e seu comportamento no modelo experimental do camundongo César Gómez-Hernández Karine Rezende-Oliveira Gabriel Antônio Nogueira Nascentes Lara Rocha Batista Henrique Borges Kappel José Alejandro Martinez-Ibarra Francisco Trujillo Contreras Eliane Lages-Silva Luis Eduardo Ramírez 2011-12-01T00:00:00Z https://doaj.org/article/ce0aa25602a54efa908a2473e89a923d EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000600007 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/ce0aa25602a54efa908a2473e89a923d Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 6, Pp 684-690 (2011) Trypanosoma cruzi México Caracterização biológica Caracterização genética Mexico Biological characterization Genetic characterization Arctic medicine. Tropical medicine RC955-962 article 2011 ftdoajarticles 2022-12-31T02:46:32Z INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI. INTRODUÇÃO: Durante muito tempo, foi questionada a importância da doença de Chagas no México onde muitos a consideravam um padecimento exótico. Considerando a grande diversidade genética existente, entre os isolados de Trypanosoma cruzi, a importância da caracterização biológica desses e o escasso número de informações sobre os aspectos clínicos e biológicos da doença de Chagas no México, o objetivo deste trabalho foi realizar a caracterização biológica e molecular de isolados de Trypanosoma ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Trypanosoma cruzi México Caracterização biológica Caracterização genética Mexico Biological characterization Genetic characterization Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Trypanosoma cruzi México Caracterização biológica Caracterização genética Mexico Biological characterization Genetic characterization Arctic medicine. Tropical medicine RC955-962 César Gómez-Hernández Karine Rezende-Oliveira Gabriel Antônio Nogueira Nascentes Lara Rocha Batista Henrique Borges Kappel José Alejandro Martinez-Ibarra Francisco Trujillo Contreras Eliane Lages-Silva Luis Eduardo Ramírez Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model Caracterização molecular de cepas mexicanas de Trypanosoma cruzi e seu comportamento no modelo experimental do camundongo |
topic_facet |
Trypanosoma cruzi México Caracterização biológica Caracterização genética Mexico Biological characterization Genetic characterization Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI. INTRODUÇÃO: Durante muito tempo, foi questionada a importância da doença de Chagas no México onde muitos a consideravam um padecimento exótico. Considerando a grande diversidade genética existente, entre os isolados de Trypanosoma cruzi, a importância da caracterização biológica desses e o escasso número de informações sobre os aspectos clínicos e biológicos da doença de Chagas no México, o objetivo deste trabalho foi realizar a caracterização biológica e molecular de isolados de Trypanosoma ... |
format |
Article in Journal/Newspaper |
author |
César Gómez-Hernández Karine Rezende-Oliveira Gabriel Antônio Nogueira Nascentes Lara Rocha Batista Henrique Borges Kappel José Alejandro Martinez-Ibarra Francisco Trujillo Contreras Eliane Lages-Silva Luis Eduardo Ramírez |
author_facet |
César Gómez-Hernández Karine Rezende-Oliveira Gabriel Antônio Nogueira Nascentes Lara Rocha Batista Henrique Borges Kappel José Alejandro Martinez-Ibarra Francisco Trujillo Contreras Eliane Lages-Silva Luis Eduardo Ramírez |
author_sort |
César Gómez-Hernández |
title |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model Caracterização molecular de cepas mexicanas de Trypanosoma cruzi e seu comportamento no modelo experimental do camundongo |
title_short |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model Caracterização molecular de cepas mexicanas de Trypanosoma cruzi e seu comportamento no modelo experimental do camundongo |
title_full |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model Caracterização molecular de cepas mexicanas de Trypanosoma cruzi e seu comportamento no modelo experimental do camundongo |
title_fullStr |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model Caracterização molecular de cepas mexicanas de Trypanosoma cruzi e seu comportamento no modelo experimental do camundongo |
title_full_unstemmed |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model Caracterização molecular de cepas mexicanas de Trypanosoma cruzi e seu comportamento no modelo experimental do camundongo |
title_sort |
molecular characterization of trypanosoma cruzi mexican strains and their behavior in the mouse experimental model caracterização molecular de cepas mexicanas de trypanosoma cruzi e seu comportamento no modelo experimental do camundongo |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2011 |
url |
https://doaj.org/article/ce0aa25602a54efa908a2473e89a923d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 6, Pp 684-690 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000600007 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/ce0aa25602a54efa908a2473e89a923d |
_version_ |
1766348144578134016 |