Dynamics of Plasmodium vivax sporogony in wild Anopheles stephensi in a malaria-endemic region of Western India
Abstract Background In global efforts to track mosquito infectivity and parasite elimination, controlled mosquito-feeding experiments can help in understanding the dynamics of parasite development in vectors. Anopheles stephensi is often accepted as the major urban malaria vector that transmits Plas...
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ftdoajarticles:oai:doaj.org/article:cda38170eb434c70bb2040e329258bea 2023-05-15T15:14:53+02:00 Dynamics of Plasmodium vivax sporogony in wild Anopheles stephensi in a malaria-endemic region of Western India Praveen Balabaskaran Nina Ajeet Kumar Mohanty Shuvankar Ballav Smita Vernekar Sushma Bhinge Maria D’souza Jayashree Walke Suresh Kumar Manoharan Anjali Mascarenhas Edwin Gomes Laura Chery Neena Valecha Ashwani Kumar Pradipsinh K. Rathod 2017-07-01T00:00:00Z https://doi.org/10.1186/s12936-017-1931-8 https://doaj.org/article/cda38170eb434c70bb2040e329258bea EN eng BMC http://link.springer.com/article/10.1186/s12936-017-1931-8 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1931-8 1475-2875 https://doaj.org/article/cda38170eb434c70bb2040e329258bea Malaria Journal, Vol 16, Iss 1, Pp 1-12 (2017) MESA-ICEMR Goa Vector infection Anopheles stephensi Plasmodium vivax Oocysts Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2017 ftdoajarticles https://doi.org/10.1186/s12936-017-1931-8 2022-12-31T08:43:55Z Abstract Background In global efforts to track mosquito infectivity and parasite elimination, controlled mosquito-feeding experiments can help in understanding the dynamics of parasite development in vectors. Anopheles stephensi is often accepted as the major urban malaria vector that transmits Plasmodium in Goa and elsewhere in South Asia. However, much needs to be learned about the interactions of Plasmodium vivax with An. stephensi. As a component of the US NIH International Center of Excellence for Malaria Research (ICEMR) for Malaria Evolution in South Asia (MESA), a series of membrane-feeding experiments with wild An. stephensi and P. vivax were carried out to better understand this vector-parasite interaction. Methods Wild An. stephensi larvae and pupae were collected from curing water in construction sites in the city of Ponda, Goa, India. The larvae and pupae were reared at the MESA ICEMR insectary within the National Institute of Malaria Research (NIMR) field unit in Goa until they emerged into adult mosquitoes. Blood for membrane-feeding experiments was obtained from malaria patients at the local Goa Medical College and Hospital who volunteered for the study. Parasites were counted by Miller reticule technique and correlation between gametocytaemia/parasitaemia and successful mosquito infection was studied. Results A weak but significant correlation was found between patient blood gametocytaemia/parasitaemia and mosquito oocyst load. No correlation was observed between gametocytaemia/parasitaemia and oocyst infection rates, and between gametocyte sex ratio and oocyst load. When it came to development of the parasite in the mosquito, a strong positive correlation was observed between oocyst midgut levels and sporozoite infection rates, and between oocyst levels and salivary gland sporozoite loads. Kinetic studies showed that sporozoites appeared in the salivary gland as early as day 7, post-infection. Conclusions This is the first study in India to carry out membrane-feeding experiments with wild An. ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 16 1 |
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MESA-ICEMR Goa Vector infection Anopheles stephensi Plasmodium vivax Oocysts Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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MESA-ICEMR Goa Vector infection Anopheles stephensi Plasmodium vivax Oocysts Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Praveen Balabaskaran Nina Ajeet Kumar Mohanty Shuvankar Ballav Smita Vernekar Sushma Bhinge Maria D’souza Jayashree Walke Suresh Kumar Manoharan Anjali Mascarenhas Edwin Gomes Laura Chery Neena Valecha Ashwani Kumar Pradipsinh K. Rathod Dynamics of Plasmodium vivax sporogony in wild Anopheles stephensi in a malaria-endemic region of Western India |
topic_facet |
MESA-ICEMR Goa Vector infection Anopheles stephensi Plasmodium vivax Oocysts Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background In global efforts to track mosquito infectivity and parasite elimination, controlled mosquito-feeding experiments can help in understanding the dynamics of parasite development in vectors. Anopheles stephensi is often accepted as the major urban malaria vector that transmits Plasmodium in Goa and elsewhere in South Asia. However, much needs to be learned about the interactions of Plasmodium vivax with An. stephensi. As a component of the US NIH International Center of Excellence for Malaria Research (ICEMR) for Malaria Evolution in South Asia (MESA), a series of membrane-feeding experiments with wild An. stephensi and P. vivax were carried out to better understand this vector-parasite interaction. Methods Wild An. stephensi larvae and pupae were collected from curing water in construction sites in the city of Ponda, Goa, India. The larvae and pupae were reared at the MESA ICEMR insectary within the National Institute of Malaria Research (NIMR) field unit in Goa until they emerged into adult mosquitoes. Blood for membrane-feeding experiments was obtained from malaria patients at the local Goa Medical College and Hospital who volunteered for the study. Parasites were counted by Miller reticule technique and correlation between gametocytaemia/parasitaemia and successful mosquito infection was studied. Results A weak but significant correlation was found between patient blood gametocytaemia/parasitaemia and mosquito oocyst load. No correlation was observed between gametocytaemia/parasitaemia and oocyst infection rates, and between gametocyte sex ratio and oocyst load. When it came to development of the parasite in the mosquito, a strong positive correlation was observed between oocyst midgut levels and sporozoite infection rates, and between oocyst levels and salivary gland sporozoite loads. Kinetic studies showed that sporozoites appeared in the salivary gland as early as day 7, post-infection. Conclusions This is the first study in India to carry out membrane-feeding experiments with wild An. ... |
format |
Article in Journal/Newspaper |
author |
Praveen Balabaskaran Nina Ajeet Kumar Mohanty Shuvankar Ballav Smita Vernekar Sushma Bhinge Maria D’souza Jayashree Walke Suresh Kumar Manoharan Anjali Mascarenhas Edwin Gomes Laura Chery Neena Valecha Ashwani Kumar Pradipsinh K. Rathod |
author_facet |
Praveen Balabaskaran Nina Ajeet Kumar Mohanty Shuvankar Ballav Smita Vernekar Sushma Bhinge Maria D’souza Jayashree Walke Suresh Kumar Manoharan Anjali Mascarenhas Edwin Gomes Laura Chery Neena Valecha Ashwani Kumar Pradipsinh K. Rathod |
author_sort |
Praveen Balabaskaran Nina |
title |
Dynamics of Plasmodium vivax sporogony in wild Anopheles stephensi in a malaria-endemic region of Western India |
title_short |
Dynamics of Plasmodium vivax sporogony in wild Anopheles stephensi in a malaria-endemic region of Western India |
title_full |
Dynamics of Plasmodium vivax sporogony in wild Anopheles stephensi in a malaria-endemic region of Western India |
title_fullStr |
Dynamics of Plasmodium vivax sporogony in wild Anopheles stephensi in a malaria-endemic region of Western India |
title_full_unstemmed |
Dynamics of Plasmodium vivax sporogony in wild Anopheles stephensi in a malaria-endemic region of Western India |
title_sort |
dynamics of plasmodium vivax sporogony in wild anopheles stephensi in a malaria-endemic region of western india |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12936-017-1931-8 https://doaj.org/article/cda38170eb434c70bb2040e329258bea |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 16, Iss 1, Pp 1-12 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12936-017-1931-8 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1931-8 1475-2875 https://doaj.org/article/cda38170eb434c70bb2040e329258bea |
op_doi |
https://doi.org/10.1186/s12936-017-1931-8 |
container_title |
Malaria Journal |
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16 |
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1 |
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1766345286548979712 |