The Fermentation of a Marine Probiotic Bacterium on Low-Cost Media Formulated with Industrial Fish Gelatin Waterstreams and Collagen Hydrolysates
Chemical effluents generated by the isolation of fish gelatin and collagen hydrolysates produced from the enzyme proteolysis of skin wastes are protein-rich substrates that could be used as nutrients in bacterial bioprocessing. In this study, the suitability of such nutrients in supporting the growt...
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2023
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ftdoajarticles:oai:doaj.org/article:cd940755742f4b5bac065b39e69a7e9c 2023-09-26T15:23:55+02:00 The Fermentation of a Marine Probiotic Bacterium on Low-Cost Media Formulated with Industrial Fish Gelatin Waterstreams and Collagen Hydrolysates José Antonio Vázquez Adrián Pedreira Iván Salmerón Dyah H. Wardhani Jesus Valcarcel 2023-08-01T00:00:00Z https://doi.org/10.3390/pr11082397 https://doaj.org/article/cd940755742f4b5bac065b39e69a7e9c EN eng MDPI AG https://www.mdpi.com/2227-9717/11/8/2397 https://doaj.org/toc/2227-9717 doi:10.3390/pr11082397 2227-9717 https://doaj.org/article/cd940755742f4b5bac065b39e69a7e9c Processes, Vol 11, Iss 2397, p 2397 (2023) Phaeobacter sp. DIFR 27-4 marine probiotic bacteria gelatin effluents collagen hydrolysates sustainability bioconversion Chemical technology TP1-1185 Chemistry QD1-999 article 2023 ftdoajarticles https://doi.org/10.3390/pr11082397 2023-08-27T00:34:57Z Chemical effluents generated by the isolation of fish gelatin and collagen hydrolysates produced from the enzyme proteolysis of skin wastes are protein-rich substrates that could be used as nutrients in bacterial bioprocessing. In this study, the suitability of such nutrients in supporting the growth of a marine probiotic bacterium, Phaeobacter sp. DIFR 27-4, was studied. Both gelatin effluents and collagen hydrolysates were obtained from the skins of shark, tuna, salmon and turbot. The chemical composition of the substrates included the complete presence of all quantified amino acids. Low-cost marine culture media were formulated with these protein materials alongside a very low concentration of yeast extract and marine water. In batch cultures with gelatin effluents, the growth rates of the strain DIFR 27-4 were somewhat lower than those found in the control marine commercial media. In the case of the hydrolysates, the bacterial production of biomass was similar or higher than that observed in the control, and larger than that observed in the effluents. A simple evaluation of production costs in the different substrates studied indicated that around a 73–125-fold reduction can be achieved when alternative media are used, in comparison to the use of commercial marine broth. Article in Journal/Newspaper Turbot Directory of Open Access Journals: DOAJ Articles Processes 11 8 2397 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Phaeobacter sp. DIFR 27-4 marine probiotic bacteria gelatin effluents collagen hydrolysates sustainability bioconversion Chemical technology TP1-1185 Chemistry QD1-999 |
spellingShingle |
Phaeobacter sp. DIFR 27-4 marine probiotic bacteria gelatin effluents collagen hydrolysates sustainability bioconversion Chemical technology TP1-1185 Chemistry QD1-999 José Antonio Vázquez Adrián Pedreira Iván Salmerón Dyah H. Wardhani Jesus Valcarcel The Fermentation of a Marine Probiotic Bacterium on Low-Cost Media Formulated with Industrial Fish Gelatin Waterstreams and Collagen Hydrolysates |
topic_facet |
Phaeobacter sp. DIFR 27-4 marine probiotic bacteria gelatin effluents collagen hydrolysates sustainability bioconversion Chemical technology TP1-1185 Chemistry QD1-999 |
description |
Chemical effluents generated by the isolation of fish gelatin and collagen hydrolysates produced from the enzyme proteolysis of skin wastes are protein-rich substrates that could be used as nutrients in bacterial bioprocessing. In this study, the suitability of such nutrients in supporting the growth of a marine probiotic bacterium, Phaeobacter sp. DIFR 27-4, was studied. Both gelatin effluents and collagen hydrolysates were obtained from the skins of shark, tuna, salmon and turbot. The chemical composition of the substrates included the complete presence of all quantified amino acids. Low-cost marine culture media were formulated with these protein materials alongside a very low concentration of yeast extract and marine water. In batch cultures with gelatin effluents, the growth rates of the strain DIFR 27-4 were somewhat lower than those found in the control marine commercial media. In the case of the hydrolysates, the bacterial production of biomass was similar or higher than that observed in the control, and larger than that observed in the effluents. A simple evaluation of production costs in the different substrates studied indicated that around a 73–125-fold reduction can be achieved when alternative media are used, in comparison to the use of commercial marine broth. |
format |
Article in Journal/Newspaper |
author |
José Antonio Vázquez Adrián Pedreira Iván Salmerón Dyah H. Wardhani Jesus Valcarcel |
author_facet |
José Antonio Vázquez Adrián Pedreira Iván Salmerón Dyah H. Wardhani Jesus Valcarcel |
author_sort |
José Antonio Vázquez |
title |
The Fermentation of a Marine Probiotic Bacterium on Low-Cost Media Formulated with Industrial Fish Gelatin Waterstreams and Collagen Hydrolysates |
title_short |
The Fermentation of a Marine Probiotic Bacterium on Low-Cost Media Formulated with Industrial Fish Gelatin Waterstreams and Collagen Hydrolysates |
title_full |
The Fermentation of a Marine Probiotic Bacterium on Low-Cost Media Formulated with Industrial Fish Gelatin Waterstreams and Collagen Hydrolysates |
title_fullStr |
The Fermentation of a Marine Probiotic Bacterium on Low-Cost Media Formulated with Industrial Fish Gelatin Waterstreams and Collagen Hydrolysates |
title_full_unstemmed |
The Fermentation of a Marine Probiotic Bacterium on Low-Cost Media Formulated with Industrial Fish Gelatin Waterstreams and Collagen Hydrolysates |
title_sort |
fermentation of a marine probiotic bacterium on low-cost media formulated with industrial fish gelatin waterstreams and collagen hydrolysates |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/pr11082397 https://doaj.org/article/cd940755742f4b5bac065b39e69a7e9c |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
Processes, Vol 11, Iss 2397, p 2397 (2023) |
op_relation |
https://www.mdpi.com/2227-9717/11/8/2397 https://doaj.org/toc/2227-9717 doi:10.3390/pr11082397 2227-9717 https://doaj.org/article/cd940755742f4b5bac065b39e69a7e9c |
op_doi |
https://doi.org/10.3390/pr11082397 |
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Processes |
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11 |
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8 |
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2397 |
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1778150281010216960 |