Transcription Profiles of Age-at-Maturity-Associated Genes Suggest Cell Fate Commitment Regulation as a Key Factor in the Atlantic Salmon Maturation Process
Despite recent taxonomic diversification in studies linking genotype with phenotype, follow-up studies aimed at understanding the molecular processes of such genotype-phenotype associations remain rare. The age at which an individual reaches sexual maturity is an important fitness trait in many wild...
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Oxford University Press
2020
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Online Access: | https://doi.org/10.1534/g3.119.400882 https://doaj.org/article/cd872fe134a84c63ad20e03cb7c3fe79 |
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ftdoajarticles:oai:doaj.org/article:cd872fe134a84c63ad20e03cb7c3fe79 2023-05-15T15:30:31+02:00 Transcription Profiles of Age-at-Maturity-Associated Genes Suggest Cell Fate Commitment Regulation as a Key Factor in the Atlantic Salmon Maturation Process Johanna Kurko Paul V. Debes Andrew H. House Tutku Aykanat Jaakko Erkinaro Craig R. Primmer 2020-01-01T00:00:00Z https://doi.org/10.1534/g3.119.400882 https://doaj.org/article/cd872fe134a84c63ad20e03cb7c3fe79 EN eng Oxford University Press http://g3journal.org/lookup/doi/10.1534/g3.119.400882 https://doaj.org/toc/2160-1836 2160-1836 doi:10.1534/g3.119.400882 https://doaj.org/article/cd872fe134a84c63ad20e03cb7c3fe79 G3: Genes, Genomes, Genetics, Vol 10, Iss 1, Pp 235-246 (2020) atlantic salmon maturation process vgll3 mrna expression cell fate regulation Genetics QH426-470 article 2020 ftdoajarticles https://doi.org/10.1534/g3.119.400882 2022-12-31T06:38:56Z Despite recent taxonomic diversification in studies linking genotype with phenotype, follow-up studies aimed at understanding the molecular processes of such genotype-phenotype associations remain rare. The age at which an individual reaches sexual maturity is an important fitness trait in many wild species. However, the molecular mechanisms regulating maturation timing processes remain obscure. A recent genome-wide association study in Atlantic salmon (Salmo salar) identified large-effect age-at-maturity-associated chromosomal regions including genes vgll3, akap11 and six6, which have roles in adipogenesis, spermatogenesis and the hypothalamic-pituitary-gonadal (HPG) axis, respectively. Here, we determine expression patterns of these genes during salmon development and their potential molecular partners and pathways. Using Nanostring transcription profiling technology, we show development- and tissue-specific mRNA expression patterns for vgll3, akap11 and six6. Correlated expression levels of vgll3 and akap11, which have adjacent chromosomal location, suggests they may have shared regulation. Further, vgll3 correlating with arhgap6 and yap1, and akap11 with lats1 and yap1 suggests that Vgll3 and Akap11 take part in actin cytoskeleton regulation. Tissue-specific expression results indicate that vgll3 and akap11 paralogs have sex-dependent expression patterns in gonads. Moreover, six6 correlating with slc38a6 and rtn1, and Hippo signaling genes suggests that Six6 could have a broader role in the HPG neuroendrocrine and cell fate commitment regulation, respectively. We conclude that Vgll3, Akap11 and Six6 may influence Atlantic salmon maturation timing via affecting adipogenesis and gametogenesis by regulating cell fate commitment and the HPG axis. These results may help to unravel general molecular mechanisms behind maturation. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles G3 Genes|Genomes|Genetics 10 1 235 246 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
atlantic salmon maturation process vgll3 mrna expression cell fate regulation Genetics QH426-470 |
spellingShingle |
atlantic salmon maturation process vgll3 mrna expression cell fate regulation Genetics QH426-470 Johanna Kurko Paul V. Debes Andrew H. House Tutku Aykanat Jaakko Erkinaro Craig R. Primmer Transcription Profiles of Age-at-Maturity-Associated Genes Suggest Cell Fate Commitment Regulation as a Key Factor in the Atlantic Salmon Maturation Process |
topic_facet |
atlantic salmon maturation process vgll3 mrna expression cell fate regulation Genetics QH426-470 |
description |
Despite recent taxonomic diversification in studies linking genotype with phenotype, follow-up studies aimed at understanding the molecular processes of such genotype-phenotype associations remain rare. The age at which an individual reaches sexual maturity is an important fitness trait in many wild species. However, the molecular mechanisms regulating maturation timing processes remain obscure. A recent genome-wide association study in Atlantic salmon (Salmo salar) identified large-effect age-at-maturity-associated chromosomal regions including genes vgll3, akap11 and six6, which have roles in adipogenesis, spermatogenesis and the hypothalamic-pituitary-gonadal (HPG) axis, respectively. Here, we determine expression patterns of these genes during salmon development and their potential molecular partners and pathways. Using Nanostring transcription profiling technology, we show development- and tissue-specific mRNA expression patterns for vgll3, akap11 and six6. Correlated expression levels of vgll3 and akap11, which have adjacent chromosomal location, suggests they may have shared regulation. Further, vgll3 correlating with arhgap6 and yap1, and akap11 with lats1 and yap1 suggests that Vgll3 and Akap11 take part in actin cytoskeleton regulation. Tissue-specific expression results indicate that vgll3 and akap11 paralogs have sex-dependent expression patterns in gonads. Moreover, six6 correlating with slc38a6 and rtn1, and Hippo signaling genes suggests that Six6 could have a broader role in the HPG neuroendrocrine and cell fate commitment regulation, respectively. We conclude that Vgll3, Akap11 and Six6 may influence Atlantic salmon maturation timing via affecting adipogenesis and gametogenesis by regulating cell fate commitment and the HPG axis. These results may help to unravel general molecular mechanisms behind maturation. |
format |
Article in Journal/Newspaper |
author |
Johanna Kurko Paul V. Debes Andrew H. House Tutku Aykanat Jaakko Erkinaro Craig R. Primmer |
author_facet |
Johanna Kurko Paul V. Debes Andrew H. House Tutku Aykanat Jaakko Erkinaro Craig R. Primmer |
author_sort |
Johanna Kurko |
title |
Transcription Profiles of Age-at-Maturity-Associated Genes Suggest Cell Fate Commitment Regulation as a Key Factor in the Atlantic Salmon Maturation Process |
title_short |
Transcription Profiles of Age-at-Maturity-Associated Genes Suggest Cell Fate Commitment Regulation as a Key Factor in the Atlantic Salmon Maturation Process |
title_full |
Transcription Profiles of Age-at-Maturity-Associated Genes Suggest Cell Fate Commitment Regulation as a Key Factor in the Atlantic Salmon Maturation Process |
title_fullStr |
Transcription Profiles of Age-at-Maturity-Associated Genes Suggest Cell Fate Commitment Regulation as a Key Factor in the Atlantic Salmon Maturation Process |
title_full_unstemmed |
Transcription Profiles of Age-at-Maturity-Associated Genes Suggest Cell Fate Commitment Regulation as a Key Factor in the Atlantic Salmon Maturation Process |
title_sort |
transcription profiles of age-at-maturity-associated genes suggest cell fate commitment regulation as a key factor in the atlantic salmon maturation process |
publisher |
Oxford University Press |
publishDate |
2020 |
url |
https://doi.org/10.1534/g3.119.400882 https://doaj.org/article/cd872fe134a84c63ad20e03cb7c3fe79 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
G3: Genes, Genomes, Genetics, Vol 10, Iss 1, Pp 235-246 (2020) |
op_relation |
http://g3journal.org/lookup/doi/10.1534/g3.119.400882 https://doaj.org/toc/2160-1836 2160-1836 doi:10.1534/g3.119.400882 https://doaj.org/article/cd872fe134a84c63ad20e03cb7c3fe79 |
op_doi |
https://doi.org/10.1534/g3.119.400882 |
container_title |
G3 Genes|Genomes|Genetics |
container_volume |
10 |
container_issue |
1 |
container_start_page |
235 |
op_container_end_page |
246 |
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1766360962135228416 |