Isolation and sequencing of Dashli virus, a novel Sicilian-like virus in sandflies from Iran; genetic and phylogenetic evidence for the creation of one novel species within the Phlebovirus genus in the Phenuiviridae family.
Phlebotomine sandflies are vectors of phleboviruses that cause sandfly fever or meningitis with significant implications for public health. Although several strains of these viruses had been isolated in Iran in the late 1970's, there was no recent data about the present situation at the outset...
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ftdoajarticles:oai:doaj.org/article:cd803c71ff294d6c84122ea30856094a 2023-05-15T15:13:48+02:00 Isolation and sequencing of Dashli virus, a novel Sicilian-like virus in sandflies from Iran; genetic and phylogenetic evidence for the creation of one novel species within the Phlebovirus genus in the Phenuiviridae family. Cigdem Alkan Vahideh Moin Vaziri Nazli Ayhan Mehdi Badakhshan Laurence Bichaud Nourina Rahbarian Ezat-Aldin Javadian Bulent Alten Xavier de Lamballerie Remi N Charrel 2017-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005978 https://doaj.org/article/cd803c71ff294d6c84122ea30856094a EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5760094?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005978 https://doaj.org/article/cd803c71ff294d6c84122ea30856094a PLoS Neglected Tropical Diseases, Vol 11, Iss 12, p e0005978 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005978 2022-12-31T16:23:10Z Phlebotomine sandflies are vectors of phleboviruses that cause sandfly fever or meningitis with significant implications for public health. Although several strains of these viruses had been isolated in Iran in the late 1970's, there was no recent data about the present situation at the outset of this study. Entomological investigations performed in 2009 and 2011 in Iran collected 4,770 sandflies from 10 different regions. Based on morphological identification, they were sorted into 315 pools according to species, sex, trapping station and date of capture. A phlebovirus, provisionally named Dashli virus (DASHV), was isolated from one pool of Sergentomyia spp, and subsequently DASHV RNA was detected in a second pool of Phlebotomus papatasi. Genetic and phylogenetic analyses based on complete coding genomic sequences indicated that (i) DASHV is most closely related to the Iranian isolates of Sandfly fever Sicilian virus [SFSV], (ii) there is a common ancestor to DASHV, Sandfly fever Sicilian- (SFS) and SFS-like viruses isolated in Italy, India, Turkey, and Cyprus (lineage I), (iii) DASHV is more distantly related with Corfou and Toros viruses (lineage II) although common ancestry is supported with 100% bootstrap, (iii) lineage I can be subdivided into sublineage Ia including all SFSV, SFCV and SFTV except those isolated in Iran which forms sublineage Ib (DASHV). Accordingly, we suggest to approve Sandfly fever Sicilian virus species consisting of the all aforementioned viruses. Owing that most of these viruses have been identified in human patients with febrile illness, DASHV should be considered as a potential human pathogen in Iran. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Toros ENVELOPE(33.417,33.417,69.300,69.300) PLOS Neglected Tropical Diseases 11 12 e0005978 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Cigdem Alkan Vahideh Moin Vaziri Nazli Ayhan Mehdi Badakhshan Laurence Bichaud Nourina Rahbarian Ezat-Aldin Javadian Bulent Alten Xavier de Lamballerie Remi N Charrel Isolation and sequencing of Dashli virus, a novel Sicilian-like virus in sandflies from Iran; genetic and phylogenetic evidence for the creation of one novel species within the Phlebovirus genus in the Phenuiviridae family. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Phlebotomine sandflies are vectors of phleboviruses that cause sandfly fever or meningitis with significant implications for public health. Although several strains of these viruses had been isolated in Iran in the late 1970's, there was no recent data about the present situation at the outset of this study. Entomological investigations performed in 2009 and 2011 in Iran collected 4,770 sandflies from 10 different regions. Based on morphological identification, they were sorted into 315 pools according to species, sex, trapping station and date of capture. A phlebovirus, provisionally named Dashli virus (DASHV), was isolated from one pool of Sergentomyia spp, and subsequently DASHV RNA was detected in a second pool of Phlebotomus papatasi. Genetic and phylogenetic analyses based on complete coding genomic sequences indicated that (i) DASHV is most closely related to the Iranian isolates of Sandfly fever Sicilian virus [SFSV], (ii) there is a common ancestor to DASHV, Sandfly fever Sicilian- (SFS) and SFS-like viruses isolated in Italy, India, Turkey, and Cyprus (lineage I), (iii) DASHV is more distantly related with Corfou and Toros viruses (lineage II) although common ancestry is supported with 100% bootstrap, (iii) lineage I can be subdivided into sublineage Ia including all SFSV, SFCV and SFTV except those isolated in Iran which forms sublineage Ib (DASHV). Accordingly, we suggest to approve Sandfly fever Sicilian virus species consisting of the all aforementioned viruses. Owing that most of these viruses have been identified in human patients with febrile illness, DASHV should be considered as a potential human pathogen in Iran. |
format |
Article in Journal/Newspaper |
author |
Cigdem Alkan Vahideh Moin Vaziri Nazli Ayhan Mehdi Badakhshan Laurence Bichaud Nourina Rahbarian Ezat-Aldin Javadian Bulent Alten Xavier de Lamballerie Remi N Charrel |
author_facet |
Cigdem Alkan Vahideh Moin Vaziri Nazli Ayhan Mehdi Badakhshan Laurence Bichaud Nourina Rahbarian Ezat-Aldin Javadian Bulent Alten Xavier de Lamballerie Remi N Charrel |
author_sort |
Cigdem Alkan |
title |
Isolation and sequencing of Dashli virus, a novel Sicilian-like virus in sandflies from Iran; genetic and phylogenetic evidence for the creation of one novel species within the Phlebovirus genus in the Phenuiviridae family. |
title_short |
Isolation and sequencing of Dashli virus, a novel Sicilian-like virus in sandflies from Iran; genetic and phylogenetic evidence for the creation of one novel species within the Phlebovirus genus in the Phenuiviridae family. |
title_full |
Isolation and sequencing of Dashli virus, a novel Sicilian-like virus in sandflies from Iran; genetic and phylogenetic evidence for the creation of one novel species within the Phlebovirus genus in the Phenuiviridae family. |
title_fullStr |
Isolation and sequencing of Dashli virus, a novel Sicilian-like virus in sandflies from Iran; genetic and phylogenetic evidence for the creation of one novel species within the Phlebovirus genus in the Phenuiviridae family. |
title_full_unstemmed |
Isolation and sequencing of Dashli virus, a novel Sicilian-like virus in sandflies from Iran; genetic and phylogenetic evidence for the creation of one novel species within the Phlebovirus genus in the Phenuiviridae family. |
title_sort |
isolation and sequencing of dashli virus, a novel sicilian-like virus in sandflies from iran; genetic and phylogenetic evidence for the creation of one novel species within the phlebovirus genus in the phenuiviridae family. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005978 https://doaj.org/article/cd803c71ff294d6c84122ea30856094a |
long_lat |
ENVELOPE(33.417,33.417,69.300,69.300) |
geographic |
Arctic Toros |
geographic_facet |
Arctic Toros |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 12, p e0005978 (2017) |
op_relation |
http://europepmc.org/articles/PMC5760094?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005978 https://doaj.org/article/cd803c71ff294d6c84122ea30856094a |
op_doi |
https://doi.org/10.1371/journal.pntd.0005978 |
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PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
12 |
container_start_page |
e0005978 |
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