Murine Models for Trypanosoma brucei gambiense disease progression--from silent to chronic infections and early brain tropism.
Human African trypanosomiasis (HAT) caused by Trypanosoma brucei gambiense remains highly prevalent in west and central Africa and is lethal if left untreated. The major problem is that the disease often evolves toward chronic or asymptomatic forms with low and fluctuating parasitaemia producing app...
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ftdoajarticles:oai:doaj.org/article:cd05c6025b534e609f8bf860d9c9bbeb 2023-05-15T15:15:16+02:00 Murine Models for Trypanosoma brucei gambiense disease progression--from silent to chronic infections and early brain tropism. Christiane Giroud Florence Ottones Virginie Coustou Denis Dacheux Nicolas Biteau Benjamin Miezan Nick Van Reet Mark Carrington Felix Doua Théo Baltz 2009-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000509 https://doaj.org/article/cd05c6025b534e609f8bf860d9c9bbeb EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2728506?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000509 https://doaj.org/article/cd05c6025b534e609f8bf860d9c9bbeb PLoS Neglected Tropical Diseases, Vol 3, Iss 9, p e509 (2009) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2009 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000509 2022-12-31T01:35:42Z Human African trypanosomiasis (HAT) caused by Trypanosoma brucei gambiense remains highly prevalent in west and central Africa and is lethal if left untreated. The major problem is that the disease often evolves toward chronic or asymptomatic forms with low and fluctuating parasitaemia producing apparently aparasitaemic serological suspects who remain untreated because of the toxicity of the chemotherapy. Whether the different types of infections are due to host or parasite factors has been difficult to address, since T. b. gambiense isolated from patients is often not infectious in rodents thus limiting the variety of isolates.T. b. gambiense parasites were outgrown directly from the cerebrospinal fluid of infected patients by in vitro culture and analyzed for their molecular polymorphisms. Experimental murine infections showed that these isolates could be clustered into three groups with different characteristics regarding their in vivo infection properties, immune response and capacity for brain invasion. The first isolate induced a classical chronic infection with a fluctuating blood parasitaemia, an invasion of the central nervous system (CNS), a trypanosome specific-antibody response and death of the animals within 6-8 months. The second group induced a sub-chronic infection resulting in a single wave of parasitaemia after infection, followed by a low parasitaemia with no parasites detected by microscope observations of blood but detected by PCR, and the presence of a specific antibody response. The third isolate induced a silent infection characterised by the absence of microscopically detectable parasites throughout, but infection was detectable by PCR during the whole course of infection. Additionally, specific antibodies were barely detectable when mice were infected with a low number of this group of parasites. In both sub-chronic and chronic infections, most of the mice survived more than one year without major clinical symptoms despite an early dissemination and growth of the parasites in different ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 3 9 e509 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Christiane Giroud Florence Ottones Virginie Coustou Denis Dacheux Nicolas Biteau Benjamin Miezan Nick Van Reet Mark Carrington Felix Doua Théo Baltz Murine Models for Trypanosoma brucei gambiense disease progression--from silent to chronic infections and early brain tropism. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Human African trypanosomiasis (HAT) caused by Trypanosoma brucei gambiense remains highly prevalent in west and central Africa and is lethal if left untreated. The major problem is that the disease often evolves toward chronic or asymptomatic forms with low and fluctuating parasitaemia producing apparently aparasitaemic serological suspects who remain untreated because of the toxicity of the chemotherapy. Whether the different types of infections are due to host or parasite factors has been difficult to address, since T. b. gambiense isolated from patients is often not infectious in rodents thus limiting the variety of isolates.T. b. gambiense parasites were outgrown directly from the cerebrospinal fluid of infected patients by in vitro culture and analyzed for their molecular polymorphisms. Experimental murine infections showed that these isolates could be clustered into three groups with different characteristics regarding their in vivo infection properties, immune response and capacity for brain invasion. The first isolate induced a classical chronic infection with a fluctuating blood parasitaemia, an invasion of the central nervous system (CNS), a trypanosome specific-antibody response and death of the animals within 6-8 months. The second group induced a sub-chronic infection resulting in a single wave of parasitaemia after infection, followed by a low parasitaemia with no parasites detected by microscope observations of blood but detected by PCR, and the presence of a specific antibody response. The third isolate induced a silent infection characterised by the absence of microscopically detectable parasites throughout, but infection was detectable by PCR during the whole course of infection. Additionally, specific antibodies were barely detectable when mice were infected with a low number of this group of parasites. In both sub-chronic and chronic infections, most of the mice survived more than one year without major clinical symptoms despite an early dissemination and growth of the parasites in different ... |
format |
Article in Journal/Newspaper |
author |
Christiane Giroud Florence Ottones Virginie Coustou Denis Dacheux Nicolas Biteau Benjamin Miezan Nick Van Reet Mark Carrington Felix Doua Théo Baltz |
author_facet |
Christiane Giroud Florence Ottones Virginie Coustou Denis Dacheux Nicolas Biteau Benjamin Miezan Nick Van Reet Mark Carrington Felix Doua Théo Baltz |
author_sort |
Christiane Giroud |
title |
Murine Models for Trypanosoma brucei gambiense disease progression--from silent to chronic infections and early brain tropism. |
title_short |
Murine Models for Trypanosoma brucei gambiense disease progression--from silent to chronic infections and early brain tropism. |
title_full |
Murine Models for Trypanosoma brucei gambiense disease progression--from silent to chronic infections and early brain tropism. |
title_fullStr |
Murine Models for Trypanosoma brucei gambiense disease progression--from silent to chronic infections and early brain tropism. |
title_full_unstemmed |
Murine Models for Trypanosoma brucei gambiense disease progression--from silent to chronic infections and early brain tropism. |
title_sort |
murine models for trypanosoma brucei gambiense disease progression--from silent to chronic infections and early brain tropism. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2009 |
url |
https://doi.org/10.1371/journal.pntd.0000509 https://doaj.org/article/cd05c6025b534e609f8bf860d9c9bbeb |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 3, Iss 9, p e509 (2009) |
op_relation |
http://europepmc.org/articles/PMC2728506?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000509 https://doaj.org/article/cd05c6025b534e609f8bf860d9c9bbeb |
op_doi |
https://doi.org/10.1371/journal.pntd.0000509 |
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PLoS Neglected Tropical Diseases |
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3 |
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9 |
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e509 |
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