The volume of the sample as a factor of survival of sturgeon spermatozoa after cryopreservation

• Published in: E3S Web of Conferences
• Main Authors: Krasilnikova Aleksandra, Ponomareva Elena, Shvedova Svetlana, Magomedov Magomed, Rudoy Dmitry, Olshevskaya Anastasiya
• Format: Article in Journal/Newspaper
• Language: English; French
• Published: EDP Sciences 2020
• Subjects: Environmental sciences; GE1-350; Acipenser baerii; Siberian sturgeon
• Online Access: https://doi.org/10.1051/e3sconf/202021007010; https://doaj.org/article/ccf2a3abc09e4953b08efceb8fc0fe9a

This research was carried out to examine the effect of various volumes (0.5, 0.75, 1.5 and 2 mL) of the frozen sample on cryopreservation of sturgeon sperm and also the possibility of using the method of vitrification of sperm under deep low-temperature cooling in the form of thin films on nets. The object of the study was the spermatozoa of the Russian sturgeon (Acipenser gueldenstaedtii Brandt, 1833) and the Siberian sturgeon of the Lena population (Acipenser baerii Brandt, 1869). There is a direct relationship between the volume of frozen material and the survival rate of defrosted sperm. With the increase in freeze sample preservation frozen-melted cells is falling, as is the range of cooling rate to freeze the sample, in which the majority of cells are frozen at a speed different from the optimal values. When cryopreservation of a sperm smear in the form of a thin film, the analysis of cell movement activity after defrosting showed the suitability of such sperm for use in the fish-breeding process. The highest life time of the sperm as it was observed during the freezing of the films on the plastic samples.