The volume of the sample as a factor of survival of sturgeon spermatozoa after cryopreservation
This research was carried out to examine the effect of various volumes (0.5, 0.75, 1.5 and 2 mL) of the frozen sample on cryopreservation of sturgeon sperm and also the possibility of using the method of vitrification of sperm under deep low-temperature cooling in the form of thin films on nets. The...
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ftdoajarticles:oai:doaj.org/article:ccf2a3abc09e4953b08efceb8fc0fe9a 2023-05-15T13:01:50+02:00 The volume of the sample as a factor of survival of sturgeon spermatozoa after cryopreservation Krasilnikova Aleksandra Ponomareva Elena Shvedova Svetlana Magomedov Magomed Rudoy Dmitry Olshevskaya Anastasiya 2020-01-01T00:00:00Z https://doi.org/10.1051/e3sconf/202021007010 https://doaj.org/article/ccf2a3abc09e4953b08efceb8fc0fe9a EN FR eng fre EDP Sciences https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/70/e3sconf_itse2020_07010.pdf https://doaj.org/toc/2267-1242 2267-1242 doi:10.1051/e3sconf/202021007010 https://doaj.org/article/ccf2a3abc09e4953b08efceb8fc0fe9a E3S Web of Conferences, Vol 210, p 07010 (2020) Environmental sciences GE1-350 article 2020 ftdoajarticles https://doi.org/10.1051/e3sconf/202021007010 2022-12-31T07:39:21Z This research was carried out to examine the effect of various volumes (0.5, 0.75, 1.5 and 2 mL) of the frozen sample on cryopreservation of sturgeon sperm and also the possibility of using the method of vitrification of sperm under deep low-temperature cooling in the form of thin films on nets. The object of the study was the spermatozoa of the Russian sturgeon (Acipenser gueldenstaedtii Brandt, 1833) and the Siberian sturgeon of the Lena population (Acipenser baerii Brandt, 1869). There is a direct relationship between the volume of frozen material and the survival rate of defrosted sperm. With the increase in freeze sample preservation frozen-melted cells is falling, as is the range of cooling rate to freeze the sample, in which the majority of cells are frozen at a speed different from the optimal values. When cryopreservation of a sperm smear in the form of a thin film, the analysis of cell movement activity after defrosting showed the suitability of such sperm for use in the fish-breeding process. The highest life time of the sperm as it was observed during the freezing of the films on the plastic samples. Article in Journal/Newspaper Acipenser baerii Siberian sturgeon Directory of Open Access Journals: DOAJ Articles E3S Web of Conferences 210 07010 |
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English French |
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Environmental sciences GE1-350 |
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Environmental sciences GE1-350 Krasilnikova Aleksandra Ponomareva Elena Shvedova Svetlana Magomedov Magomed Rudoy Dmitry Olshevskaya Anastasiya The volume of the sample as a factor of survival of sturgeon spermatozoa after cryopreservation |
topic_facet |
Environmental sciences GE1-350 |
description |
This research was carried out to examine the effect of various volumes (0.5, 0.75, 1.5 and 2 mL) of the frozen sample on cryopreservation of sturgeon sperm and also the possibility of using the method of vitrification of sperm under deep low-temperature cooling in the form of thin films on nets. The object of the study was the spermatozoa of the Russian sturgeon (Acipenser gueldenstaedtii Brandt, 1833) and the Siberian sturgeon of the Lena population (Acipenser baerii Brandt, 1869). There is a direct relationship between the volume of frozen material and the survival rate of defrosted sperm. With the increase in freeze sample preservation frozen-melted cells is falling, as is the range of cooling rate to freeze the sample, in which the majority of cells are frozen at a speed different from the optimal values. When cryopreservation of a sperm smear in the form of a thin film, the analysis of cell movement activity after defrosting showed the suitability of such sperm for use in the fish-breeding process. The highest life time of the sperm as it was observed during the freezing of the films on the plastic samples. |
format |
Article in Journal/Newspaper |
author |
Krasilnikova Aleksandra Ponomareva Elena Shvedova Svetlana Magomedov Magomed Rudoy Dmitry Olshevskaya Anastasiya |
author_facet |
Krasilnikova Aleksandra Ponomareva Elena Shvedova Svetlana Magomedov Magomed Rudoy Dmitry Olshevskaya Anastasiya |
author_sort |
Krasilnikova Aleksandra |
title |
The volume of the sample as a factor of survival of sturgeon spermatozoa after cryopreservation |
title_short |
The volume of the sample as a factor of survival of sturgeon spermatozoa after cryopreservation |
title_full |
The volume of the sample as a factor of survival of sturgeon spermatozoa after cryopreservation |
title_fullStr |
The volume of the sample as a factor of survival of sturgeon spermatozoa after cryopreservation |
title_full_unstemmed |
The volume of the sample as a factor of survival of sturgeon spermatozoa after cryopreservation |
title_sort |
volume of the sample as a factor of survival of sturgeon spermatozoa after cryopreservation |
publisher |
EDP Sciences |
publishDate |
2020 |
url |
https://doi.org/10.1051/e3sconf/202021007010 https://doaj.org/article/ccf2a3abc09e4953b08efceb8fc0fe9a |
genre |
Acipenser baerii Siberian sturgeon |
genre_facet |
Acipenser baerii Siberian sturgeon |
op_source |
E3S Web of Conferences, Vol 210, p 07010 (2020) |
op_relation |
https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/70/e3sconf_itse2020_07010.pdf https://doaj.org/toc/2267-1242 2267-1242 doi:10.1051/e3sconf/202021007010 https://doaj.org/article/ccf2a3abc09e4953b08efceb8fc0fe9a |
op_doi |
https://doi.org/10.1051/e3sconf/202021007010 |
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E3S Web of Conferences |
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210 |
container_start_page |
07010 |
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1766288068857298944 |