Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast
Thirteen individual organochlorine compounds at 3 concentrations (80, 400, and 2000 ng/mL culture medium), as well as mixtures, were assayed for the estrogen receptor (ER) activation or inhibition, using a luciferase reporter gene assay (RGA). None of the PCB 138, 153, or 180 or their mixture induce...
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ftdoajarticles:oai:doaj.org/article:ccd8a33c5f0b4ba48b8f1509912acc9e 2023-05-15T16:33:26+02:00 Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast Pedro Henrique Imazaki François Brose Thierry Jauniaux Krishna Das Marc Muller Marie-Louise Scippo 2015-01-01T00:00:00Z https://doi.org/10.1155/2015/438295 https://doaj.org/article/ccd8a33c5f0b4ba48b8f1509912acc9e EN eng Hindawi Limited http://dx.doi.org/10.1155/2015/438295 https://doaj.org/toc/2314-6133 https://doaj.org/toc/2314-6141 2314-6133 2314-6141 doi:10.1155/2015/438295 https://doaj.org/article/ccd8a33c5f0b4ba48b8f1509912acc9e BioMed Research International, Vol 2015 (2015) Medicine R article 2015 ftdoajarticles https://doi.org/10.1155/2015/438295 2022-12-31T14:55:07Z Thirteen individual organochlorine compounds at 3 concentrations (80, 400, and 2000 ng/mL culture medium), as well as mixtures, were assayed for the estrogen receptor (ER) activation or inhibition, using a luciferase reporter gene assay (RGA). None of the PCB 138, 153, or 180 or their mixture induced a response in the RGA. o,p′-DDT was the most potent xenoestrogen from the DDT group, inducing a response already at 80 ng/mL. From the HCH and HCB group, only β-HCH (at 400 and 2000 ng/mL) and δ-HCH (at 2000 ng/mL) displayed estrogenic activities. These 13 organochlorines were determined by GC-MS in 12 samples of North Sea harbor porpoise blubber. The PCBs were the main contaminants. Within each group, PCB 153 (6.0 × 102~4.2 × 104 μg/kg), p,p′-DDE (5.1 × 102~8.6 × 103 μg/kg), and HCB (7.6 × 101~1.5 × 103 μg/kg) were the compounds found in highest concentrations. The hormonal activity of the porpoise blubber samples was also assayed in RGA, where two samples showed estrogenic activity, seven samples showed antiestrogenic activity, and one sample showed both estrogenic and antiestrogenic activity. Our results suggest that the 13 POPs measured by GC-MS in the samples cannot explain alone the estrogenicity of the extracts. Article in Journal/Newspaper Harbour porpoise Phocoena phocoena Directory of Open Access Journals: DOAJ Articles BioMed Research International 2015 1 13 |
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English |
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Medicine R Pedro Henrique Imazaki François Brose Thierry Jauniaux Krishna Das Marc Muller Marie-Louise Scippo Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast |
topic_facet |
Medicine R |
description |
Thirteen individual organochlorine compounds at 3 concentrations (80, 400, and 2000 ng/mL culture medium), as well as mixtures, were assayed for the estrogen receptor (ER) activation or inhibition, using a luciferase reporter gene assay (RGA). None of the PCB 138, 153, or 180 or their mixture induced a response in the RGA. o,p′-DDT was the most potent xenoestrogen from the DDT group, inducing a response already at 80 ng/mL. From the HCH and HCB group, only β-HCH (at 400 and 2000 ng/mL) and δ-HCH (at 2000 ng/mL) displayed estrogenic activities. These 13 organochlorines were determined by GC-MS in 12 samples of North Sea harbor porpoise blubber. The PCBs were the main contaminants. Within each group, PCB 153 (6.0 × 102~4.2 × 104 μg/kg), p,p′-DDE (5.1 × 102~8.6 × 103 μg/kg), and HCB (7.6 × 101~1.5 × 103 μg/kg) were the compounds found in highest concentrations. The hormonal activity of the porpoise blubber samples was also assayed in RGA, where two samples showed estrogenic activity, seven samples showed antiestrogenic activity, and one sample showed both estrogenic and antiestrogenic activity. Our results suggest that the 13 POPs measured by GC-MS in the samples cannot explain alone the estrogenicity of the extracts. |
format |
Article in Journal/Newspaper |
author |
Pedro Henrique Imazaki François Brose Thierry Jauniaux Krishna Das Marc Muller Marie-Louise Scippo |
author_facet |
Pedro Henrique Imazaki François Brose Thierry Jauniaux Krishna Das Marc Muller Marie-Louise Scippo |
author_sort |
Pedro Henrique Imazaki |
title |
Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast |
title_short |
Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast |
title_full |
Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast |
title_fullStr |
Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast |
title_full_unstemmed |
Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast |
title_sort |
estrogenic evaluation and organochlorine identification in blubber of north sea harbour porpoise (phocoena phocoena) stranded on the north sea coast |
publisher |
Hindawi Limited |
publishDate |
2015 |
url |
https://doi.org/10.1155/2015/438295 https://doaj.org/article/ccd8a33c5f0b4ba48b8f1509912acc9e |
genre |
Harbour porpoise Phocoena phocoena |
genre_facet |
Harbour porpoise Phocoena phocoena |
op_source |
BioMed Research International, Vol 2015 (2015) |
op_relation |
http://dx.doi.org/10.1155/2015/438295 https://doaj.org/toc/2314-6133 https://doaj.org/toc/2314-6141 2314-6133 2314-6141 doi:10.1155/2015/438295 https://doaj.org/article/ccd8a33c5f0b4ba48b8f1509912acc9e |
op_doi |
https://doi.org/10.1155/2015/438295 |
container_title |
BioMed Research International |
container_volume |
2015 |
container_start_page |
1 |
op_container_end_page |
13 |
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1766023125723512832 |