Genome-wide identification of Aedes albopictus long noncoding RNAs and their association with dengue and Zika virus infection.
The Asian tiger mosquito, Aedes albopictus (Ae. albopictus), is an important vector that transmits arboviruses such as dengue (DENV), Zika (ZIKV) and Chikungunya virus (CHIKV). Long noncoding RNAs (lncRNAs) are known to regulate various biological processes. Knowledge on Ae. albopictus lncRNAs and t...
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ftdoajarticles:oai:doaj.org/article:cca3f26d1ce6446b9b257f20bb51a9d6 2023-05-15T15:11:15+02:00 Genome-wide identification of Aedes albopictus long noncoding RNAs and their association with dengue and Zika virus infection. Azali Azlan Sattam M Obeidat Kumitaa Theva Das Muhammad Amir Yunus Ghows Azzam 2021-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008351 https://doaj.org/article/cca3f26d1ce6446b9b257f20bb51a9d6 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008351 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008351 https://doaj.org/article/cca3f26d1ce6446b9b257f20bb51a9d6 PLoS Neglected Tropical Diseases, Vol 15, Iss 1, p e0008351 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008351 2022-12-31T07:16:57Z The Asian tiger mosquito, Aedes albopictus (Ae. albopictus), is an important vector that transmits arboviruses such as dengue (DENV), Zika (ZIKV) and Chikungunya virus (CHIKV). Long noncoding RNAs (lncRNAs) are known to regulate various biological processes. Knowledge on Ae. albopictus lncRNAs and their functional role in virus-host interactions are still limited. Here, we identified and characterized the lncRNAs in the genome of an arbovirus vector, Ae. albopictus, and evaluated their potential involvement in DENV and ZIKV infection. We used 148 public datasets, and identified a total of 10, 867 novel lncRNA transcripts, of which 5,809, 4,139, and 919 were intergenic, intronic and antisense respectively. The Ae. albopictus lncRNAs shared many characteristics with other species such as short length, low GC content, and low sequence conservation. RNA-sequencing of Ae. albopictus cells infected with DENV and ZIKV showed that the expression of lncRNAs was altered upon virus infection. Target prediction analysis revealed that Ae. albopictus lncRNAs may regulate the expression of genes involved in immunity and other metabolic and cellular processes. To verify the role of lncRNAs in virus infection, we generated mutations in lncRNA loci using CRISPR-Cas9, and discovered that two lncRNA loci mutations, namely XLOC_029733 (novel lncRNA transcript id: lncRNA_27639.2) and LOC115270134 (known lncRNA transcript id: XR_003899061.1) resulted in enhancement of DENV and ZIKV replication. The results presented here provide an important foundation for future studies of lncRNAs and their relationship with virus infection in Ae. albopictus. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 1 e0008351 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Azali Azlan Sattam M Obeidat Kumitaa Theva Das Muhammad Amir Yunus Ghows Azzam Genome-wide identification of Aedes albopictus long noncoding RNAs and their association with dengue and Zika virus infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The Asian tiger mosquito, Aedes albopictus (Ae. albopictus), is an important vector that transmits arboviruses such as dengue (DENV), Zika (ZIKV) and Chikungunya virus (CHIKV). Long noncoding RNAs (lncRNAs) are known to regulate various biological processes. Knowledge on Ae. albopictus lncRNAs and their functional role in virus-host interactions are still limited. Here, we identified and characterized the lncRNAs in the genome of an arbovirus vector, Ae. albopictus, and evaluated their potential involvement in DENV and ZIKV infection. We used 148 public datasets, and identified a total of 10, 867 novel lncRNA transcripts, of which 5,809, 4,139, and 919 were intergenic, intronic and antisense respectively. The Ae. albopictus lncRNAs shared many characteristics with other species such as short length, low GC content, and low sequence conservation. RNA-sequencing of Ae. albopictus cells infected with DENV and ZIKV showed that the expression of lncRNAs was altered upon virus infection. Target prediction analysis revealed that Ae. albopictus lncRNAs may regulate the expression of genes involved in immunity and other metabolic and cellular processes. To verify the role of lncRNAs in virus infection, we generated mutations in lncRNA loci using CRISPR-Cas9, and discovered that two lncRNA loci mutations, namely XLOC_029733 (novel lncRNA transcript id: lncRNA_27639.2) and LOC115270134 (known lncRNA transcript id: XR_003899061.1) resulted in enhancement of DENV and ZIKV replication. The results presented here provide an important foundation for future studies of lncRNAs and their relationship with virus infection in Ae. albopictus. |
format |
Article in Journal/Newspaper |
author |
Azali Azlan Sattam M Obeidat Kumitaa Theva Das Muhammad Amir Yunus Ghows Azzam |
author_facet |
Azali Azlan Sattam M Obeidat Kumitaa Theva Das Muhammad Amir Yunus Ghows Azzam |
author_sort |
Azali Azlan |
title |
Genome-wide identification of Aedes albopictus long noncoding RNAs and their association with dengue and Zika virus infection. |
title_short |
Genome-wide identification of Aedes albopictus long noncoding RNAs and their association with dengue and Zika virus infection. |
title_full |
Genome-wide identification of Aedes albopictus long noncoding RNAs and their association with dengue and Zika virus infection. |
title_fullStr |
Genome-wide identification of Aedes albopictus long noncoding RNAs and their association with dengue and Zika virus infection. |
title_full_unstemmed |
Genome-wide identification of Aedes albopictus long noncoding RNAs and their association with dengue and Zika virus infection. |
title_sort |
genome-wide identification of aedes albopictus long noncoding rnas and their association with dengue and zika virus infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0008351 https://doaj.org/article/cca3f26d1ce6446b9b257f20bb51a9d6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 1, p e0008351 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008351 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008351 https://doaj.org/article/cca3f26d1ce6446b9b257f20bb51a9d6 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008351 |
container_title |
PLOS Neglected Tropical Diseases |
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15 |
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1 |
container_start_page |
e0008351 |
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1766342138385137664 |