Estimation of time detection limit for human cytochrome b in females of Lutzomyia evansi
Introduction: Molecular biology techniques have allowed a better knowledge of sources of blood meals in vector insects. However, the usefulness of these techniques depends on both the quantity of ingested blood and the digestion process in the insect. Objective: To identify the time limit for detect...
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ftdoajarticles:oai:doaj.org/article:cc7c20efead84c898bc5fa5dde59b0e4 2023-05-15T15:09:40+02:00 Estimation of time detection limit for human cytochrome b in females of Lutzomyia evansi José Gabriel Vergara Daniel Verbel-Vergara Ana Milena Montesino Alveiro Pérez-Doria Eduar Elías Bejarano 2017-03-01T00:00:00Z https://doi.org/10.7705/biomedica.v37i0.3396 https://doaj.org/article/cc7c20efead84c898bc5fa5dde59b0e4 EN ES eng spa Instituto Nacional de Salud https://www.revistabiomedica.org/index.php/biomedica/article/view/3396 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v37i0.3396 https://doaj.org/article/cc7c20efead84c898bc5fa5dde59b0e4 Biomédica: revista del Instituto Nacional de Salud, Vol 37, Iss 0, Pp 187-192 (2017) Psychodidae cytochrome b insect vectors blood leishmaniasis Medicine R Arctic medicine. Tropical medicine RC955-962 article 2017 ftdoajarticles https://doi.org/10.7705/biomedica.v37i0.3396 2022-12-30T23:47:58Z Introduction: Molecular biology techniques have allowed a better knowledge of sources of blood meals in vector insects. However, the usefulness of these techniques depends on both the quantity of ingested blood and the digestion process in the insect. Objective: To identify the time limit for detection of the human cytochrome b (Cyt b) gene in experimentally fed females of Lutzomyia evansi. Materials and methods: Eight groups of L. evansi females were fed on human blood and sacrificed at intervals of 24 hours post-ingestion. Total DNA was extracted from each female and a segment of 358 bp of Cyt b was amplified. In order to eliminate false positives, amplification products were subjected to a restriction fragment length polymorphism (RFLP) analysis. Results: The human Cyt b gene segment was detected in 86% (49/57) of the females of L. evansi, from 0 to 168 hours after blood ingestion. In 7% (4/57) of the individuals we amplified insect DNA, while in the remaining 7%, the band of interest was not amplified. We did not find any statistical differences between groups of females sacrificed at different times post-blood meal regarding the amplification of the human Cyt b gene segment or the number of samples amplified. Conclusion: The human Cyt b gene segment was detectable in L. evansi females up to 168 hours after blood ingestion. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 37 187 |
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Directory of Open Access Journals: DOAJ Articles |
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English Spanish |
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Psychodidae cytochrome b insect vectors blood leishmaniasis Medicine R Arctic medicine. Tropical medicine RC955-962 |
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Psychodidae cytochrome b insect vectors blood leishmaniasis Medicine R Arctic medicine. Tropical medicine RC955-962 José Gabriel Vergara Daniel Verbel-Vergara Ana Milena Montesino Alveiro Pérez-Doria Eduar Elías Bejarano Estimation of time detection limit for human cytochrome b in females of Lutzomyia evansi |
topic_facet |
Psychodidae cytochrome b insect vectors blood leishmaniasis Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: Molecular biology techniques have allowed a better knowledge of sources of blood meals in vector insects. However, the usefulness of these techniques depends on both the quantity of ingested blood and the digestion process in the insect. Objective: To identify the time limit for detection of the human cytochrome b (Cyt b) gene in experimentally fed females of Lutzomyia evansi. Materials and methods: Eight groups of L. evansi females were fed on human blood and sacrificed at intervals of 24 hours post-ingestion. Total DNA was extracted from each female and a segment of 358 bp of Cyt b was amplified. In order to eliminate false positives, amplification products were subjected to a restriction fragment length polymorphism (RFLP) analysis. Results: The human Cyt b gene segment was detected in 86% (49/57) of the females of L. evansi, from 0 to 168 hours after blood ingestion. In 7% (4/57) of the individuals we amplified insect DNA, while in the remaining 7%, the band of interest was not amplified. We did not find any statistical differences between groups of females sacrificed at different times post-blood meal regarding the amplification of the human Cyt b gene segment or the number of samples amplified. Conclusion: The human Cyt b gene segment was detectable in L. evansi females up to 168 hours after blood ingestion. |
format |
Article in Journal/Newspaper |
author |
José Gabriel Vergara Daniel Verbel-Vergara Ana Milena Montesino Alveiro Pérez-Doria Eduar Elías Bejarano |
author_facet |
José Gabriel Vergara Daniel Verbel-Vergara Ana Milena Montesino Alveiro Pérez-Doria Eduar Elías Bejarano |
author_sort |
José Gabriel Vergara |
title |
Estimation of time detection limit for human cytochrome b in females of Lutzomyia evansi |
title_short |
Estimation of time detection limit for human cytochrome b in females of Lutzomyia evansi |
title_full |
Estimation of time detection limit for human cytochrome b in females of Lutzomyia evansi |
title_fullStr |
Estimation of time detection limit for human cytochrome b in females of Lutzomyia evansi |
title_full_unstemmed |
Estimation of time detection limit for human cytochrome b in females of Lutzomyia evansi |
title_sort |
estimation of time detection limit for human cytochrome b in females of lutzomyia evansi |
publisher |
Instituto Nacional de Salud |
publishDate |
2017 |
url |
https://doi.org/10.7705/biomedica.v37i0.3396 https://doaj.org/article/cc7c20efead84c898bc5fa5dde59b0e4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 37, Iss 0, Pp 187-192 (2017) |
op_relation |
https://www.revistabiomedica.org/index.php/biomedica/article/view/3396 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v37i0.3396 https://doaj.org/article/cc7c20efead84c898bc5fa5dde59b0e4 |
op_doi |
https://doi.org/10.7705/biomedica.v37i0.3396 |
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Biomédica |
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37 |
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