Genetic typing of isolates of Rickettsia typhi.
Murine typhus, which is caused by Rickettsia typhi, has a wide range of clinical manifestations. It has a low mortality rate but may result in meningoencephalitis and interstitial pneumonia in severe cases. Comparisons of complete genome sequences of R. typhi isolates from North Carolina, USA (Wilmi...
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ftdoajarticles:oai:doaj.org/article:cc3919fbaf10468298a98ddf89ef4e06 2023-05-15T15:09:44+02:00 Genetic typing of isolates of Rickettsia typhi. Cecilia Y Kato Ida H Chung Lauren K Robinson Marina E Eremeeva Gregory A Dasch 2022-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010354 https://doaj.org/article/cc3919fbaf10468298a98ddf89ef4e06 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010354 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010354 https://doaj.org/article/cc3919fbaf10468298a98ddf89ef4e06 PLoS Neglected Tropical Diseases, Vol 16, Iss 5, p e0010354 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010354 2022-12-31T02:24:01Z Murine typhus, which is caused by Rickettsia typhi, has a wide range of clinical manifestations. It has a low mortality rate but may result in meningoencephalitis and interstitial pneumonia in severe cases. Comparisons of complete genome sequences of R. typhi isolates from North Carolina, USA (Wilmington), Myanmar (B9991PP), and Thailand (TH1527) identified only 26 single nucleotide polymorphism (SNP) and 7 insertion-deletion (INDEL) sites in these highly syntenic genomes. Assays were developed to further define the distribution of these variant sites among 15 additional isolates of R. typhi with different histories from Asia, the USA, and Africa. Mismatch amplification mutation assays (MAMA) were validated for 22 SNP sites, while the 7 INDEL sites were analyzed directly on agarose gels. Six SNP types, 9 INDEL types, 11 total types were identified among these 18 isolates. Replicate DNA samples as well as comparisons of isolates with different passage and source histories gave consistent genetic typing profiles. Comparison of the SNP and INDEL markers to R. typhi's nearest neighbor Rickettsia prowazekii demonstrated that the majority of the SNPs represent intra-species variation that arose post divergence of these two species while several INDEL sites also exhibited intraspecies variability among the R. prowazekii genomes that have been completely sequenced. The assays for the presence of these SNP and INDEL sites, particularly the latter, comprise a low technology gel method for consistently distinguishing R. typhi and R. prowazekii as well as for differentiating genetic types of R. typhi. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Indel’ ENVELOPE(35.282,35.282,66.963,66.963) PLOS Neglected Tropical Diseases 16 5 e0010354 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Cecilia Y Kato Ida H Chung Lauren K Robinson Marina E Eremeeva Gregory A Dasch Genetic typing of isolates of Rickettsia typhi. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Murine typhus, which is caused by Rickettsia typhi, has a wide range of clinical manifestations. It has a low mortality rate but may result in meningoencephalitis and interstitial pneumonia in severe cases. Comparisons of complete genome sequences of R. typhi isolates from North Carolina, USA (Wilmington), Myanmar (B9991PP), and Thailand (TH1527) identified only 26 single nucleotide polymorphism (SNP) and 7 insertion-deletion (INDEL) sites in these highly syntenic genomes. Assays were developed to further define the distribution of these variant sites among 15 additional isolates of R. typhi with different histories from Asia, the USA, and Africa. Mismatch amplification mutation assays (MAMA) were validated for 22 SNP sites, while the 7 INDEL sites were analyzed directly on agarose gels. Six SNP types, 9 INDEL types, 11 total types were identified among these 18 isolates. Replicate DNA samples as well as comparisons of isolates with different passage and source histories gave consistent genetic typing profiles. Comparison of the SNP and INDEL markers to R. typhi's nearest neighbor Rickettsia prowazekii demonstrated that the majority of the SNPs represent intra-species variation that arose post divergence of these two species while several INDEL sites also exhibited intraspecies variability among the R. prowazekii genomes that have been completely sequenced. The assays for the presence of these SNP and INDEL sites, particularly the latter, comprise a low technology gel method for consistently distinguishing R. typhi and R. prowazekii as well as for differentiating genetic types of R. typhi. |
format |
Article in Journal/Newspaper |
author |
Cecilia Y Kato Ida H Chung Lauren K Robinson Marina E Eremeeva Gregory A Dasch |
author_facet |
Cecilia Y Kato Ida H Chung Lauren K Robinson Marina E Eremeeva Gregory A Dasch |
author_sort |
Cecilia Y Kato |
title |
Genetic typing of isolates of Rickettsia typhi. |
title_short |
Genetic typing of isolates of Rickettsia typhi. |
title_full |
Genetic typing of isolates of Rickettsia typhi. |
title_fullStr |
Genetic typing of isolates of Rickettsia typhi. |
title_full_unstemmed |
Genetic typing of isolates of Rickettsia typhi. |
title_sort |
genetic typing of isolates of rickettsia typhi. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010354 https://doaj.org/article/cc3919fbaf10468298a98ddf89ef4e06 |
long_lat |
ENVELOPE(35.282,35.282,66.963,66.963) |
geographic |
Arctic Indel’ |
geographic_facet |
Arctic Indel’ |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 5, p e0010354 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010354 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010354 https://doaj.org/article/cc3919fbaf10468298a98ddf89ef4e06 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010354 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
5 |
container_start_page |
e0010354 |
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