pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas)
Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and sa...
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The Company of Biologists
2018
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ftdoajarticles:oai:doaj.org/article:cc30332644a1479595f377160336a1cb 2023-05-15T15:58:01+02:00 pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) Myrina Boulais Marc Suquet Eve Julie Arsenault-Pernet Florent Malo Isabelle Queau Patricia Pignet Dominique Ratiskol Jacqueline Le Grand Matthias Huber Jacky Cosson 2018-03-01T00:00:00Z https://doi.org/10.1242/bio.031427 https://doaj.org/article/cc30332644a1479595f377160336a1cb EN eng The Company of Biologists http://bio.biologists.org/content/7/3/bio031427 https://doaj.org/toc/2046-6390 2046-6390 doi:10.1242/bio.031427 https://doaj.org/article/cc30332644a1479595f377160336a1cb Biology Open, Vol 7, Iss 3 (2018) Seminal fluid Spermatozoa Motility pH Salinity Ions Science Q Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.1242/bio.031427 2022-12-31T06:50:41Z Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster, Crassostrea gigas. The acidic pH of the gonad (5.82±0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared to NH4Cl-free SW, revealing the role of pHi in triggering sperm movement. Percentage of motile spermatozoa activated in Na+-free artificial seawater (ASW) was highly reduced compared to ASW, suggesting that change of pHi triggering sperm motility was mediated by a Na+/H+ exchanger. Motility and swimming speed were highest in salinities between 33.8 and 42.7‰ (within a range of 0 to 50 ‰), and pH values above 7.5 (within a range of 4.5 to 9.5). Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Biology Open |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Seminal fluid Spermatozoa Motility pH Salinity Ions Science Q Biology (General) QH301-705.5 |
spellingShingle |
Seminal fluid Spermatozoa Motility pH Salinity Ions Science Q Biology (General) QH301-705.5 Myrina Boulais Marc Suquet Eve Julie Arsenault-Pernet Florent Malo Isabelle Queau Patricia Pignet Dominique Ratiskol Jacqueline Le Grand Matthias Huber Jacky Cosson pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
topic_facet |
Seminal fluid Spermatozoa Motility pH Salinity Ions Science Q Biology (General) QH301-705.5 |
description |
Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster, Crassostrea gigas. The acidic pH of the gonad (5.82±0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared to NH4Cl-free SW, revealing the role of pHi in triggering sperm movement. Percentage of motile spermatozoa activated in Na+-free artificial seawater (ASW) was highly reduced compared to ASW, suggesting that change of pHi triggering sperm motility was mediated by a Na+/H+ exchanger. Motility and swimming speed were highest in salinities between 33.8 and 42.7‰ (within a range of 0 to 50 ‰), and pH values above 7.5 (within a range of 4.5 to 9.5). |
format |
Article in Journal/Newspaper |
author |
Myrina Boulais Marc Suquet Eve Julie Arsenault-Pernet Florent Malo Isabelle Queau Patricia Pignet Dominique Ratiskol Jacqueline Le Grand Matthias Huber Jacky Cosson |
author_facet |
Myrina Boulais Marc Suquet Eve Julie Arsenault-Pernet Florent Malo Isabelle Queau Patricia Pignet Dominique Ratiskol Jacqueline Le Grand Matthias Huber Jacky Cosson |
author_sort |
Myrina Boulais |
title |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_short |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_full |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_fullStr |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_full_unstemmed |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_sort |
ph controls spermatozoa motility in the pacific oyster (crassostrea gigas) |
publisher |
The Company of Biologists |
publishDate |
2018 |
url |
https://doi.org/10.1242/bio.031427 https://doaj.org/article/cc30332644a1479595f377160336a1cb |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Biology Open, Vol 7, Iss 3 (2018) |
op_relation |
http://bio.biologists.org/content/7/3/bio031427 https://doaj.org/toc/2046-6390 2046-6390 doi:10.1242/bio.031427 https://doaj.org/article/cc30332644a1479595f377160336a1cb |
op_doi |
https://doi.org/10.1242/bio.031427 |
container_title |
Biology Open |
_version_ |
1766393744363356160 |