Molecular Detection of 10 of the Most Unwanted Alien Forest Pathogens in Canada Using Real-Time PCR.
Invasive alien tree pathogens can cause significant economic losses as well as large-scale damage to natural ecosystems. Early detection to prevent their establishment and spread is an important approach used by several national plant protection organizations (NPPOs). Molecular detection tools targe...
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ftdoajarticles:oai:doaj.org/article:cc1dc84269004d3db0676c816ad88387 2023-05-15T18:30:55+02:00 Molecular Detection of 10 of the Most Unwanted Alien Forest Pathogens in Canada Using Real-Time PCR. Josyanne Lamarche Amélie Potvin Gervais Pelletier Don Stewart Nicolas Feau Dario I O Alayon Angela L Dale Aaron Coelho Adnan Uzunovic Guillaume J Bilodeau Stephan C Brière Richard C Hamelin Philippe Tanguay 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0134265 https://doaj.org/article/cc1dc84269004d3db0676c816ad88387 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pone.0134265 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0134265 https://doaj.org/article/cc1dc84269004d3db0676c816ad88387 PLoS ONE, Vol 10, Iss 8, p e0134265 (2015) Medicine R Science Q article 2015 ftdoajarticles https://doi.org/10.1371/journal.pone.0134265 2022-12-31T12:50:41Z Invasive alien tree pathogens can cause significant economic losses as well as large-scale damage to natural ecosystems. Early detection to prevent their establishment and spread is an important approach used by several national plant protection organizations (NPPOs). Molecular detection tools targeting 10 of the most unwanted alien forest pathogens in Canada were developed as part of the TAIGA project (http://taigaforesthealth.com/). Forest pathogens were selected following an independent prioritization. Specific TaqMan real-time PCR detection assays were designed to function under homogeneous conditions so that they may be used in 96- or 384-well plate format arrays for high-throughput testing of large numbers of samples against multiple targets. Assays were validated for 1) specificity, 2) sensitivity, 3) precision, and 4) robustness on environmental samples. All assays were highly specific when evaluated against a panel of pure cultures of target and phylogenetically closely-related species. Sensitivity, evaluated by assessing the limit of detection (with a threshold of 95% of positive samples), was found to be between one and ten target gene region copies. Precision or repeatability of each assay revealed a mean coefficient of variation of 3.4%. All assays successfully allowed detection of target pathogen on positive environmental samples, without any non-specific amplification. These molecular detection tools will allow for rapid and reliable detection of 10 of the most unwanted alien forest pathogens in Canada. Article in Journal/Newspaper taiga Directory of Open Access Journals: DOAJ Articles Canada PLOS ONE 10 8 e0134265 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Josyanne Lamarche Amélie Potvin Gervais Pelletier Don Stewart Nicolas Feau Dario I O Alayon Angela L Dale Aaron Coelho Adnan Uzunovic Guillaume J Bilodeau Stephan C Brière Richard C Hamelin Philippe Tanguay Molecular Detection of 10 of the Most Unwanted Alien Forest Pathogens in Canada Using Real-Time PCR. |
topic_facet |
Medicine R Science Q |
description |
Invasive alien tree pathogens can cause significant economic losses as well as large-scale damage to natural ecosystems. Early detection to prevent their establishment and spread is an important approach used by several national plant protection organizations (NPPOs). Molecular detection tools targeting 10 of the most unwanted alien forest pathogens in Canada were developed as part of the TAIGA project (http://taigaforesthealth.com/). Forest pathogens were selected following an independent prioritization. Specific TaqMan real-time PCR detection assays were designed to function under homogeneous conditions so that they may be used in 96- or 384-well plate format arrays for high-throughput testing of large numbers of samples against multiple targets. Assays were validated for 1) specificity, 2) sensitivity, 3) precision, and 4) robustness on environmental samples. All assays were highly specific when evaluated against a panel of pure cultures of target and phylogenetically closely-related species. Sensitivity, evaluated by assessing the limit of detection (with a threshold of 95% of positive samples), was found to be between one and ten target gene region copies. Precision or repeatability of each assay revealed a mean coefficient of variation of 3.4%. All assays successfully allowed detection of target pathogen on positive environmental samples, without any non-specific amplification. These molecular detection tools will allow for rapid and reliable detection of 10 of the most unwanted alien forest pathogens in Canada. |
format |
Article in Journal/Newspaper |
author |
Josyanne Lamarche Amélie Potvin Gervais Pelletier Don Stewart Nicolas Feau Dario I O Alayon Angela L Dale Aaron Coelho Adnan Uzunovic Guillaume J Bilodeau Stephan C Brière Richard C Hamelin Philippe Tanguay |
author_facet |
Josyanne Lamarche Amélie Potvin Gervais Pelletier Don Stewart Nicolas Feau Dario I O Alayon Angela L Dale Aaron Coelho Adnan Uzunovic Guillaume J Bilodeau Stephan C Brière Richard C Hamelin Philippe Tanguay |
author_sort |
Josyanne Lamarche |
title |
Molecular Detection of 10 of the Most Unwanted Alien Forest Pathogens in Canada Using Real-Time PCR. |
title_short |
Molecular Detection of 10 of the Most Unwanted Alien Forest Pathogens in Canada Using Real-Time PCR. |
title_full |
Molecular Detection of 10 of the Most Unwanted Alien Forest Pathogens in Canada Using Real-Time PCR. |
title_fullStr |
Molecular Detection of 10 of the Most Unwanted Alien Forest Pathogens in Canada Using Real-Time PCR. |
title_full_unstemmed |
Molecular Detection of 10 of the Most Unwanted Alien Forest Pathogens in Canada Using Real-Time PCR. |
title_sort |
molecular detection of 10 of the most unwanted alien forest pathogens in canada using real-time pcr. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pone.0134265 https://doaj.org/article/cc1dc84269004d3db0676c816ad88387 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
taiga |
genre_facet |
taiga |
op_source |
PLoS ONE, Vol 10, Iss 8, p e0134265 (2015) |
op_relation |
https://doi.org/10.1371/journal.pone.0134265 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0134265 https://doaj.org/article/cc1dc84269004d3db0676c816ad88387 |
op_doi |
https://doi.org/10.1371/journal.pone.0134265 |
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PLOS ONE |
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10 |
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8 |
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e0134265 |
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