Identification of commonly regulated genes in HPV18- and HPV16-infected cervical cancer cells treated with the curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one
Objective: To identify commonly regulated genes in HPV-infected HeLa and CaSki cervical cancer cells treated with curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one (MS17) and to explore potential mechanisms that underlie its cytotoxic, anti-proliferative and apoptotic activity. Methods...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2018
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Online Access: | https://doi.org/10.4103/2221-1691.221137 https://doaj.org/article/cc197421403f43c6aaa07ed5b27a3be5 |
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ftdoajarticles:oai:doaj.org/article:cc197421403f43c6aaa07ed5b27a3be5 2023-05-15T15:14:28+02:00 Identification of commonly regulated genes in HPV18- and HPV16-infected cervical cancer cells treated with the curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one Felicia Paulraj Faridah Abas Nordin H Lajis Iekhsan Othman Sharifah Syed Hassan Rakesh Naidu 2018-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.221137 https://doaj.org/article/cc197421403f43c6aaa07ed5b27a3be5 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=1;spage=44;epage=51;aulast=Paulraj https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.221137 https://doaj.org/article/cc197421403f43c6aaa07ed5b27a3be5 Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 1, Pp 44-51 (2018) diarylpentanoid cervical cancer gene expression profiling microarray curcumin analogue Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.4103/2221-1691.221137 2022-12-31T00:40:23Z Objective: To identify commonly regulated genes in HPV-infected HeLa and CaSki cervical cancer cells treated with curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one (MS17) and to explore potential mechanisms that underlie its cytotoxic, anti-proliferative and apoptotic activity. Methods: HeLa and CaSki cells were treated with 2 × EC50 and 3 × EC50 doses of MS17 for 24 h and the RNA extracts were subjected to one-colour microarray-based gene expression profiling. Pair-wise significant genes (false discovery rate-corrected, P<0.05) were analysed for fold change (FC) compared to control samples. Differentially expressed genes with FC⩾2.0 (up-regulated; FC⩾2.0 and down-regulated; FC⩽-2.0) compared to the control samples were filtered through and analysed to create a global gene expression profile. Mutually regulated genes were ranked by FC and categorised by gene ontology. Results: Our data indicated dose-dependent regulation by MS17 and identified top 20 mutually up- and down-regulated genes each in HeLa and CaSki cells. Amongst these 17 were commonly regulated in both cell lines. These include the up-regulation of CCL26, DEFB103B, IL1RL1, LY96, GCNT3, MMP10, MMP3, GADD45G and HSPA6, and the down-regulation of TENM2, NEBL, KIFC1, CTDSP1, IGFBP5, LTBP1, NREP and MXD3. These genes were associated with key biological functions that were proposed to mediate the anticancer activity of MS17 in cervical cancer cells such as immune response, metabolic processes, proteolysis, programmed cell death, unfolded protein response, cell adhesion, cytoskeletal organisation, phosphatase activity, signal transduction and transcription regulator activity. Conclusions: Identification of seventeen common genes modulated by MS17 could be used as potential therapeutic targets in both cervical cancer cell lines and the findings of this study could be used to present an insight into the potential antitumor activity of MS17 in cervical cancer. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 8 1 44 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
diarylpentanoid cervical cancer gene expression profiling microarray curcumin analogue Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
diarylpentanoid cervical cancer gene expression profiling microarray curcumin analogue Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Felicia Paulraj Faridah Abas Nordin H Lajis Iekhsan Othman Sharifah Syed Hassan Rakesh Naidu Identification of commonly regulated genes in HPV18- and HPV16-infected cervical cancer cells treated with the curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one |
topic_facet |
diarylpentanoid cervical cancer gene expression profiling microarray curcumin analogue Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To identify commonly regulated genes in HPV-infected HeLa and CaSki cervical cancer cells treated with curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one (MS17) and to explore potential mechanisms that underlie its cytotoxic, anti-proliferative and apoptotic activity. Methods: HeLa and CaSki cells were treated with 2 × EC50 and 3 × EC50 doses of MS17 for 24 h and the RNA extracts were subjected to one-colour microarray-based gene expression profiling. Pair-wise significant genes (false discovery rate-corrected, P<0.05) were analysed for fold change (FC) compared to control samples. Differentially expressed genes with FC⩾2.0 (up-regulated; FC⩾2.0 and down-regulated; FC⩽-2.0) compared to the control samples were filtered through and analysed to create a global gene expression profile. Mutually regulated genes were ranked by FC and categorised by gene ontology. Results: Our data indicated dose-dependent regulation by MS17 and identified top 20 mutually up- and down-regulated genes each in HeLa and CaSki cells. Amongst these 17 were commonly regulated in both cell lines. These include the up-regulation of CCL26, DEFB103B, IL1RL1, LY96, GCNT3, MMP10, MMP3, GADD45G and HSPA6, and the down-regulation of TENM2, NEBL, KIFC1, CTDSP1, IGFBP5, LTBP1, NREP and MXD3. These genes were associated with key biological functions that were proposed to mediate the anticancer activity of MS17 in cervical cancer cells such as immune response, metabolic processes, proteolysis, programmed cell death, unfolded protein response, cell adhesion, cytoskeletal organisation, phosphatase activity, signal transduction and transcription regulator activity. Conclusions: Identification of seventeen common genes modulated by MS17 could be used as potential therapeutic targets in both cervical cancer cell lines and the findings of this study could be used to present an insight into the potential antitumor activity of MS17 in cervical cancer. |
format |
Article in Journal/Newspaper |
author |
Felicia Paulraj Faridah Abas Nordin H Lajis Iekhsan Othman Sharifah Syed Hassan Rakesh Naidu |
author_facet |
Felicia Paulraj Faridah Abas Nordin H Lajis Iekhsan Othman Sharifah Syed Hassan Rakesh Naidu |
author_sort |
Felicia Paulraj |
title |
Identification of commonly regulated genes in HPV18- and HPV16-infected cervical cancer cells treated with the curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one |
title_short |
Identification of commonly regulated genes in HPV18- and HPV16-infected cervical cancer cells treated with the curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one |
title_full |
Identification of commonly regulated genes in HPV18- and HPV16-infected cervical cancer cells treated with the curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one |
title_fullStr |
Identification of commonly regulated genes in HPV18- and HPV16-infected cervical cancer cells treated with the curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one |
title_full_unstemmed |
Identification of commonly regulated genes in HPV18- and HPV16-infected cervical cancer cells treated with the curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one |
title_sort |
identification of commonly regulated genes in hpv18- and hpv16-infected cervical cancer cells treated with the curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/2221-1691.221137 https://doaj.org/article/cc197421403f43c6aaa07ed5b27a3be5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 1, Pp 44-51 (2018) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=1;spage=44;epage=51;aulast=Paulraj https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.221137 https://doaj.org/article/cc197421403f43c6aaa07ed5b27a3be5 |
op_doi |
https://doi.org/10.4103/2221-1691.221137 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
8 |
container_issue |
1 |
container_start_page |
44 |
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1766344918810230784 |