Drug resistance to sulphadoxine-pyrimethamine in Plasmodium falciparum malaria in Mlimba, Tanzania
Abstract Background Sulphadoxine-pyrimethamine (SP) has been and is currently used for treatment of uncomplicated Plasmodium falciparum malaria in many African countries. Nevertheless, the response of parasites to SP treatment has shown significant variation between individuals. Methods The genes fo...
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ftdoajarticles:oai:doaj.org/article:cc02a1a768804309af7f3347f75f5c22 2023-05-15T15:13:17+02:00 Drug resistance to sulphadoxine-pyrimethamine in Plasmodium falciparum malaria in Mlimba, Tanzania Balthazary Sakurani T Malisa Allen L Mutayoba Benezeth M Mbugi Erasto V Nyambo Thomas B Mshinda Hassan 2006-10-01T00:00:00Z https://doi.org/10.1186/1475-2875-5-94 https://doaj.org/article/cc02a1a768804309af7f3347f75f5c22 EN eng BMC http://www.malariajournal.com/content/5/1/94 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-5-94 1475-2875 https://doaj.org/article/cc02a1a768804309af7f3347f75f5c22 Malaria Journal, Vol 5, Iss 1, p 94 (2006) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2006 ftdoajarticles https://doi.org/10.1186/1475-2875-5-94 2022-12-31T08:06:08Z Abstract Background Sulphadoxine-pyrimethamine (SP) has been and is currently used for treatment of uncomplicated Plasmodium falciparum malaria in many African countries. Nevertheless, the response of parasites to SP treatment has shown significant variation between individuals. Methods The genes for dihydrofolate reductase ( dhfr ) and dihydropteroate synthase ( dhps ) were used as markers, to investigate parasite resistance to SP in 141 children aged less than 5 years. Parasite DNA was extracted by Chelex method from blood samples collected and preserved on filter papers. Subsequently, polymerase chain reaction (PCR) and restriction fragment length polymorphism (PCR-RFLP) were applied to detect the SP resistance-associated point mutations on dhfr and dhps . Commonly reported point mutations at codons 51, 59, 108 and 164 in the dhfr and codons 437, 540 and 581 in the dhps domains were examined. Results Children infected with parasites harbouring a range of single to quintuple dhfr / dhps mutations were erratically cured with SP. However, the quintuple dhfr / dhps mutant genotypes were mostly associated with treatment failures. High proportion of SP resistance-associated point mutations was detected in this study but the adequate clinical response (89.4%) observed clinically at day 14 of follow up reflects the role of semi-immunity protection and parasite clearance in the population. Conclusion In monitoring drug resistance to SP, concurrent studies on possible confounding factors pertaining to development of resistance in falciparum malaria should be considered. The SP resistance potential detected in this study, cautions on its useful therapeutic life as an interim first-line drug against malaria in Tanzania and other malaria-endemic countries. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 5 1 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Balthazary Sakurani T Malisa Allen L Mutayoba Benezeth M Mbugi Erasto V Nyambo Thomas B Mshinda Hassan Drug resistance to sulphadoxine-pyrimethamine in Plasmodium falciparum malaria in Mlimba, Tanzania |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Sulphadoxine-pyrimethamine (SP) has been and is currently used for treatment of uncomplicated Plasmodium falciparum malaria in many African countries. Nevertheless, the response of parasites to SP treatment has shown significant variation between individuals. Methods The genes for dihydrofolate reductase ( dhfr ) and dihydropteroate synthase ( dhps ) were used as markers, to investigate parasite resistance to SP in 141 children aged less than 5 years. Parasite DNA was extracted by Chelex method from blood samples collected and preserved on filter papers. Subsequently, polymerase chain reaction (PCR) and restriction fragment length polymorphism (PCR-RFLP) were applied to detect the SP resistance-associated point mutations on dhfr and dhps . Commonly reported point mutations at codons 51, 59, 108 and 164 in the dhfr and codons 437, 540 and 581 in the dhps domains were examined. Results Children infected with parasites harbouring a range of single to quintuple dhfr / dhps mutations were erratically cured with SP. However, the quintuple dhfr / dhps mutant genotypes were mostly associated with treatment failures. High proportion of SP resistance-associated point mutations was detected in this study but the adequate clinical response (89.4%) observed clinically at day 14 of follow up reflects the role of semi-immunity protection and parasite clearance in the population. Conclusion In monitoring drug resistance to SP, concurrent studies on possible confounding factors pertaining to development of resistance in falciparum malaria should be considered. The SP resistance potential detected in this study, cautions on its useful therapeutic life as an interim first-line drug against malaria in Tanzania and other malaria-endemic countries. |
format |
Article in Journal/Newspaper |
author |
Balthazary Sakurani T Malisa Allen L Mutayoba Benezeth M Mbugi Erasto V Nyambo Thomas B Mshinda Hassan |
author_facet |
Balthazary Sakurani T Malisa Allen L Mutayoba Benezeth M Mbugi Erasto V Nyambo Thomas B Mshinda Hassan |
author_sort |
Balthazary Sakurani T |
title |
Drug resistance to sulphadoxine-pyrimethamine in Plasmodium falciparum malaria in Mlimba, Tanzania |
title_short |
Drug resistance to sulphadoxine-pyrimethamine in Plasmodium falciparum malaria in Mlimba, Tanzania |
title_full |
Drug resistance to sulphadoxine-pyrimethamine in Plasmodium falciparum malaria in Mlimba, Tanzania |
title_fullStr |
Drug resistance to sulphadoxine-pyrimethamine in Plasmodium falciparum malaria in Mlimba, Tanzania |
title_full_unstemmed |
Drug resistance to sulphadoxine-pyrimethamine in Plasmodium falciparum malaria in Mlimba, Tanzania |
title_sort |
drug resistance to sulphadoxine-pyrimethamine in plasmodium falciparum malaria in mlimba, tanzania |
publisher |
BMC |
publishDate |
2006 |
url |
https://doi.org/10.1186/1475-2875-5-94 https://doaj.org/article/cc02a1a768804309af7f3347f75f5c22 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 5, Iss 1, p 94 (2006) |
op_relation |
http://www.malariajournal.com/content/5/1/94 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-5-94 1475-2875 https://doaj.org/article/cc02a1a768804309af7f3347f75f5c22 |
op_doi |
https://doi.org/10.1186/1475-2875-5-94 |
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Malaria Journal |
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