Nosocomial infection and characterization of extended-spectrum β-lactamases-producing Enterobacteriaceae in Northeast Brazil
INTRODUCTION: Extended spectrum β-lactamases (ESBLs) are enzymes that degrade β-lactam antibiotics and have been reported to be an important cause of nosocomial infection in worldwide. METHODS: During 2009, 659 enterobacteria strains were isolated from different clinical specimens and tested for ESB...
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ftdoajarticles:oai:doaj.org/article:cbf809a855b94f2c945edebc21dc833c 2023-05-15T15:05:58+02:00 Nosocomial infection and characterization of extended-spectrum β-lactamases-producing Enterobacteriaceae in Northeast Brazil Afonso Gomes Abreu Sirlei Garcia Marques Valério Monteiro-Neto Roberto Morais Luz de Carvalho Azizedite Guedes Gonçalves 2011-08-01T00:00:00Z https://doi.org/10.1590/S0037-86822011000400008 https://doaj.org/article/cbf809a855b94f2c945edebc21dc833c EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000400008&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822011000400008 https://doaj.org/article/cbf809a855b94f2c945edebc21dc833c Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 4, Pp 441-446 (2011) Enterobactérias Resistência antimicrobiana ESBLs Infecção hospitalar Arctic medicine. Tropical medicine RC955-962 article 2011 ftdoajarticles https://doi.org/10.1590/S0037-86822011000400008 2022-12-30T21:27:05Z INTRODUCTION: Extended spectrum β-lactamases (ESBLs) are enzymes that degrade β-lactam antibiotics and have been reported to be an important cause of nosocomial infection in worldwide. METHODS: During 2009, 659 enterobacteria strains were isolated from different clinical specimens and tested for ESBL production. The disk approximation test, combined disk method and addition of clavulanic acid were used for phenotypic detection of the ESBL-producing strains and PCR for detection of the blaTEM and blaCTX-M genes. RESULTS: Among the isolates, 125 were ESBL producers. The blaCTX-M and blaTEM genes were detected in 90.4% and 75% of the strains, respectively. Most strains were isolated from urine. Klebsiella pneumoniae was the most prevalent organism. Microorganisms presented high resistance to the antibiotics. CONCLUSIONS: These results support the need for extending ESBL detection methods to different pathogens of the Enterobacteriaceae family because these methods are only currently standardized by the CLSI for Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca and Proteus mirabilis. Carbapenems were the antibiotic class of choice for the treatment of infections caused by ESBL-producing Enterobacteriaceae. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 44 4 441 446 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Enterobactérias Resistência antimicrobiana ESBLs Infecção hospitalar Arctic medicine. Tropical medicine RC955-962 |
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Enterobactérias Resistência antimicrobiana ESBLs Infecção hospitalar Arctic medicine. Tropical medicine RC955-962 Afonso Gomes Abreu Sirlei Garcia Marques Valério Monteiro-Neto Roberto Morais Luz de Carvalho Azizedite Guedes Gonçalves Nosocomial infection and characterization of extended-spectrum β-lactamases-producing Enterobacteriaceae in Northeast Brazil |
topic_facet |
Enterobactérias Resistência antimicrobiana ESBLs Infecção hospitalar Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: Extended spectrum β-lactamases (ESBLs) are enzymes that degrade β-lactam antibiotics and have been reported to be an important cause of nosocomial infection in worldwide. METHODS: During 2009, 659 enterobacteria strains were isolated from different clinical specimens and tested for ESBL production. The disk approximation test, combined disk method and addition of clavulanic acid were used for phenotypic detection of the ESBL-producing strains and PCR for detection of the blaTEM and blaCTX-M genes. RESULTS: Among the isolates, 125 were ESBL producers. The blaCTX-M and blaTEM genes were detected in 90.4% and 75% of the strains, respectively. Most strains were isolated from urine. Klebsiella pneumoniae was the most prevalent organism. Microorganisms presented high resistance to the antibiotics. CONCLUSIONS: These results support the need for extending ESBL detection methods to different pathogens of the Enterobacteriaceae family because these methods are only currently standardized by the CLSI for Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca and Proteus mirabilis. Carbapenems were the antibiotic class of choice for the treatment of infections caused by ESBL-producing Enterobacteriaceae. |
format |
Article in Journal/Newspaper |
author |
Afonso Gomes Abreu Sirlei Garcia Marques Valério Monteiro-Neto Roberto Morais Luz de Carvalho Azizedite Guedes Gonçalves |
author_facet |
Afonso Gomes Abreu Sirlei Garcia Marques Valério Monteiro-Neto Roberto Morais Luz de Carvalho Azizedite Guedes Gonçalves |
author_sort |
Afonso Gomes Abreu |
title |
Nosocomial infection and characterization of extended-spectrum β-lactamases-producing Enterobacteriaceae in Northeast Brazil |
title_short |
Nosocomial infection and characterization of extended-spectrum β-lactamases-producing Enterobacteriaceae in Northeast Brazil |
title_full |
Nosocomial infection and characterization of extended-spectrum β-lactamases-producing Enterobacteriaceae in Northeast Brazil |
title_fullStr |
Nosocomial infection and characterization of extended-spectrum β-lactamases-producing Enterobacteriaceae in Northeast Brazil |
title_full_unstemmed |
Nosocomial infection and characterization of extended-spectrum β-lactamases-producing Enterobacteriaceae in Northeast Brazil |
title_sort |
nosocomial infection and characterization of extended-spectrum β-lactamases-producing enterobacteriaceae in northeast brazil |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2011 |
url |
https://doi.org/10.1590/S0037-86822011000400008 https://doaj.org/article/cbf809a855b94f2c945edebc21dc833c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 4, Pp 441-446 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000400008&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822011000400008 https://doaj.org/article/cbf809a855b94f2c945edebc21dc833c |
op_doi |
https://doi.org/10.1590/S0037-86822011000400008 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
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44 |
container_issue |
4 |
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441 |
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446 |
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