Co-occurrence of Fe and P stress in natural populations of the marine diazotroph Trichodesmium
Trichodesmium is a globally important marine microbe that provides fixed nitrogen (N) to otherwise N-limited ecosystems. In nature, nitrogen fixation is likely regulated by iron or phosphate availability, but the extent and interaction of these controls are unclear. From metaproteomics analyses usin...
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ftdoajarticles:oai:doaj.org/article:cbceff42c8874c5eae375020ef04a920 2023-05-15T17:33:20+02:00 Co-occurrence of Fe and P stress in natural populations of the marine diazotroph Trichodesmium N. A. Held E. A. Webb M. M. McIlvin D. A. Hutchins N. R. Cohen D. M. Moran K. Kunde M. C. Lohan C. Mahaffey E. M. S. Woodward M. A. Saito 2020-05-01T00:00:00Z https://doi.org/10.5194/bg-17-2537-2020 https://doaj.org/article/cbceff42c8874c5eae375020ef04a920 EN eng Copernicus Publications https://www.biogeosciences.net/17/2537/2020/bg-17-2537-2020.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-17-2537-2020 1726-4170 1726-4189 https://doaj.org/article/cbceff42c8874c5eae375020ef04a920 Biogeosciences, Vol 17, Pp 2537-2551 (2020) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2020 ftdoajarticles https://doi.org/10.5194/bg-17-2537-2020 2022-12-31T01:08:51Z Trichodesmium is a globally important marine microbe that provides fixed nitrogen (N) to otherwise N-limited ecosystems. In nature, nitrogen fixation is likely regulated by iron or phosphate availability, but the extent and interaction of these controls are unclear. From metaproteomics analyses using established protein biomarkers for nutrient stress, we found that iron–phosphate co-stress is the norm rather than the exception for Trichodesmium colonies in the North Atlantic Ocean. Counterintuitively, the nitrogenase enzyme was more abundant under co-stress as opposed to single nutrient stress. This is consistent with the idea that Trichodesmium has a specific physiological state during nutrient co-stress. Organic nitrogen uptake was observed and occurred simultaneously with nitrogen fixation. The quantification of the phosphate ABC transporter PstA combined with a cellular model of nutrient uptake suggested that Trichodesmium is generally confronted by the biophysical limits of membrane space and diffusion rates for iron and phosphate acquisition in the field. Colony formation may benefit nutrient acquisition from particulate and organic sources, alleviating these pressures. The results highlight that to predict the behavior of Trichodesmium , both Fe and P stress must be evaluated simultaneously. Article in Journal/Newspaper North Atlantic Directory of Open Access Journals: DOAJ Articles Biogeosciences 17 9 2537 2551 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
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Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 N. A. Held E. A. Webb M. M. McIlvin D. A. Hutchins N. R. Cohen D. M. Moran K. Kunde M. C. Lohan C. Mahaffey E. M. S. Woodward M. A. Saito Co-occurrence of Fe and P stress in natural populations of the marine diazotroph Trichodesmium |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
Trichodesmium is a globally important marine microbe that provides fixed nitrogen (N) to otherwise N-limited ecosystems. In nature, nitrogen fixation is likely regulated by iron or phosphate availability, but the extent and interaction of these controls are unclear. From metaproteomics analyses using established protein biomarkers for nutrient stress, we found that iron–phosphate co-stress is the norm rather than the exception for Trichodesmium colonies in the North Atlantic Ocean. Counterintuitively, the nitrogenase enzyme was more abundant under co-stress as opposed to single nutrient stress. This is consistent with the idea that Trichodesmium has a specific physiological state during nutrient co-stress. Organic nitrogen uptake was observed and occurred simultaneously with nitrogen fixation. The quantification of the phosphate ABC transporter PstA combined with a cellular model of nutrient uptake suggested that Trichodesmium is generally confronted by the biophysical limits of membrane space and diffusion rates for iron and phosphate acquisition in the field. Colony formation may benefit nutrient acquisition from particulate and organic sources, alleviating these pressures. The results highlight that to predict the behavior of Trichodesmium , both Fe and P stress must be evaluated simultaneously. |
format |
Article in Journal/Newspaper |
author |
N. A. Held E. A. Webb M. M. McIlvin D. A. Hutchins N. R. Cohen D. M. Moran K. Kunde M. C. Lohan C. Mahaffey E. M. S. Woodward M. A. Saito |
author_facet |
N. A. Held E. A. Webb M. M. McIlvin D. A. Hutchins N. R. Cohen D. M. Moran K. Kunde M. C. Lohan C. Mahaffey E. M. S. Woodward M. A. Saito |
author_sort |
N. A. Held |
title |
Co-occurrence of Fe and P stress in natural populations of the marine diazotroph Trichodesmium |
title_short |
Co-occurrence of Fe and P stress in natural populations of the marine diazotroph Trichodesmium |
title_full |
Co-occurrence of Fe and P stress in natural populations of the marine diazotroph Trichodesmium |
title_fullStr |
Co-occurrence of Fe and P stress in natural populations of the marine diazotroph Trichodesmium |
title_full_unstemmed |
Co-occurrence of Fe and P stress in natural populations of the marine diazotroph Trichodesmium |
title_sort |
co-occurrence of fe and p stress in natural populations of the marine diazotroph trichodesmium |
publisher |
Copernicus Publications |
publishDate |
2020 |
url |
https://doi.org/10.5194/bg-17-2537-2020 https://doaj.org/article/cbceff42c8874c5eae375020ef04a920 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Biogeosciences, Vol 17, Pp 2537-2551 (2020) |
op_relation |
https://www.biogeosciences.net/17/2537/2020/bg-17-2537-2020.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-17-2537-2020 1726-4170 1726-4189 https://doaj.org/article/cbceff42c8874c5eae375020ef04a920 |
op_doi |
https://doi.org/10.5194/bg-17-2537-2020 |
container_title |
Biogeosciences |
container_volume |
17 |
container_issue |
9 |
container_start_page |
2537 |
op_container_end_page |
2551 |
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1766131814312706048 |