Chromosomal assembly of the Antarctic toothfish (Dissostichus mawsoni) genome using third-generation DNA sequencing and Hi-C technology

• Published in: Zoological Research
• Main Authors: Seung Jae Lee, Jeong-Hoon Kim, Euna Jo, Eunkyung Choi, Jinmu Kim, Seok-Gwan Choi, Sangdeok Chung, Hyun-Woo Kim, Hyun Park
• Format: Article in Journal/Newspaper
• Language: English
• Published: Science Press, PR China 2021
• Subjects: antarctic toothfish; dissostichus mawsoni; genome assembly; pacific biosciences; hi-c; Zoology; QL1-991; Antarctic; Southern Ocean; The Antarctic; Pacific; Antarc*; Antarctic Toothfish
• Online Access: https://doi.org/10.24272/j.issn.2095-8137.2020.264; https://doaj.org/article/cbc22d068365448186c4e767880898ef

The Antarctic toothfish, Dissostichus mawsoni, belongs to the Nototheniidae family and is distributed in sub-zero temperatures below S60° latitude in the Southern Ocean. Therefore, it is an attractive model species to study the stenothermal cold-adapted character state. In this study, we successfully generated highly contiguous genome sequences of D. mawsoni, which contained 1 062 scaffolds with a N50 length of 36.98 Mb and longest scaffold length of 46.82 Mb. Repetitive elements accounted for 40.87% of the genome. We also inferred 32 914 protein-coding genes using in silico gene prediction and transcriptome sequencing and detected splicing variants using Isoform-Sequencing (Iso-Seq), which will be invaluable resource for further exploration of the adaptation mechanisms of Antarctic toothfish. This new high-quality reference genome of D. mawsoni provides a fundamental resource for a deeper understanding of cold adaptation and conservation of species.