Amplicon sequencing based profiling of bacterial diversity from Krossfjorden, Arctic

In this study, Illumina Miseq sequencing of 16S rRNA gene amplicon was performed on sediments collected from Krossfjorden, Arctic for analyzing the bacterial community structure. Metagenome contained 15,936 sequences with 5,809,491 bp size and 53% G+C content. Metagenome sequence information are now...

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Bibliographic Details
Published in:Data in Brief
Main Authors: Bhavya Kachiprath, Jayesh Puthumana, Jayanath Gopi, Solly Solomon, K.P. Krishnan, Rosamma Philip
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2018
Subjects:
Computer applications to medicine. Medical informatics
R858-859.7
Science (General)
Q1-390
Arctic
Krossfjorden
Krossfjord*
Online Access:https://doi.org/10.1016/j.dib.2018.11.101
https://doaj.org/article/cbbafeffeefa4e84a9193eaaa4b8e58f
Description
Summary:In this study, Illumina Miseq sequencing of 16S rRNA gene amplicon was performed on sediments collected from Krossfjorden, Arctic for analyzing the bacterial community structure. Metagenome contained 15,936 sequences with 5,809,491 bp size and 53% G+C content. Metagenome sequence information are now available at NCBI under the Sequence Read Archive (SRA) database with accession no. SRP159159. Taxonomic hits distribution from MG-RAST analysis revealed the dominance of Alpha- and Gamma-subdivisions of Proteobacteria (88.89%) along with Bacteriodetes (8.89%) and Firmicutes (2.22%). Predominant species were Alteromonadales bacterium TW-7 (24%), Pseudoalteromonas haloplanktis (20%) and Pseudoalteromonas spp. SM9913 (18%). MG-RAST assisted analysis also detected the presence of a variety of marine taxa like Bacteriodes, Pseudovibrio, Marinobacter, Idiomarina, Teredinibacter, etc. which take part in key ecological functions and biogeochemical activities of Arctic fjord ecosystems. Keywords: Arctic, Bacterial diversity, Metagenome, Amplicon sequencing, Illumina

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