Characterization of a yeast interfering RNA larvicide with a target site conserved in the synaptotagmin gene of multiple disease vector mosquitoes.
New mosquito control strategies are vitally needed to address established and emerging arthropod-borne infectious diseases. Here we describe the characterization of a yeast interfering RNA larvicide that was developed through the genetic engineering of Saccharomyces cerevisiae (baker's yeast) t...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2019
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0007422 https://doaj.org/article/cbb70bccd19f4a0c9f5a983c96210642 |
id |
ftdoajarticles:oai:doaj.org/article:cbb70bccd19f4a0c9f5a983c96210642 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:cbb70bccd19f4a0c9f5a983c96210642 2023-05-15T15:06:21+02:00 Characterization of a yeast interfering RNA larvicide with a target site conserved in the synaptotagmin gene of multiple disease vector mosquitoes. Keshava Mysore Ping Li Chien-Wei Wang Limb K Hapairai Nicholas D Scheel Jacob S Realey Longhua Sun Joseph B Roethele David W Severson Na Wei Molly Duman-Scheel 2019-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007422 https://doaj.org/article/cbb70bccd19f4a0c9f5a983c96210642 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007422 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007422 https://doaj.org/article/cbb70bccd19f4a0c9f5a983c96210642 PLoS Neglected Tropical Diseases, Vol 13, Iss 5, p e0007422 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007422 2022-12-31T07:46:37Z New mosquito control strategies are vitally needed to address established and emerging arthropod-borne infectious diseases. Here we describe the characterization of a yeast interfering RNA larvicide that was developed through the genetic engineering of Saccharomyces cerevisiae (baker's yeast) to express a short hairpin RNA targeting the Aedes aegypti synaptotagmin (Aae syt) gene. The larvicide effectively silences the Aae syt gene, causes defects at the larval neural synapse, and induces high rates of A. aegypti larval mortality in laboratory, simulated-field, and semi-field trials. Conservation of the interfering RNA target site in multiple mosquito species, but not in humans or other non-target species, suggested that it may function as a broad-range mosquito larvicide. In support of this, consumption of the yeast interfering RNA larvicide was also found to induce high rates of larval mortality in Aedes albopictus, Anopheles gambiae, and Culex quinquefasciatus mosquito larvae. The results of these studies suggest that this biorational yeast interfering RNA larvicide may represent a new intervention that can be used to combat multiple mosquito vectors of human diseases. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 5 e0007422 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Keshava Mysore Ping Li Chien-Wei Wang Limb K Hapairai Nicholas D Scheel Jacob S Realey Longhua Sun Joseph B Roethele David W Severson Na Wei Molly Duman-Scheel Characterization of a yeast interfering RNA larvicide with a target site conserved in the synaptotagmin gene of multiple disease vector mosquitoes. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
New mosquito control strategies are vitally needed to address established and emerging arthropod-borne infectious diseases. Here we describe the characterization of a yeast interfering RNA larvicide that was developed through the genetic engineering of Saccharomyces cerevisiae (baker's yeast) to express a short hairpin RNA targeting the Aedes aegypti synaptotagmin (Aae syt) gene. The larvicide effectively silences the Aae syt gene, causes defects at the larval neural synapse, and induces high rates of A. aegypti larval mortality in laboratory, simulated-field, and semi-field trials. Conservation of the interfering RNA target site in multiple mosquito species, but not in humans or other non-target species, suggested that it may function as a broad-range mosquito larvicide. In support of this, consumption of the yeast interfering RNA larvicide was also found to induce high rates of larval mortality in Aedes albopictus, Anopheles gambiae, and Culex quinquefasciatus mosquito larvae. The results of these studies suggest that this biorational yeast interfering RNA larvicide may represent a new intervention that can be used to combat multiple mosquito vectors of human diseases. |
format |
Article in Journal/Newspaper |
author |
Keshava Mysore Ping Li Chien-Wei Wang Limb K Hapairai Nicholas D Scheel Jacob S Realey Longhua Sun Joseph B Roethele David W Severson Na Wei Molly Duman-Scheel |
author_facet |
Keshava Mysore Ping Li Chien-Wei Wang Limb K Hapairai Nicholas D Scheel Jacob S Realey Longhua Sun Joseph B Roethele David W Severson Na Wei Molly Duman-Scheel |
author_sort |
Keshava Mysore |
title |
Characterization of a yeast interfering RNA larvicide with a target site conserved in the synaptotagmin gene of multiple disease vector mosquitoes. |
title_short |
Characterization of a yeast interfering RNA larvicide with a target site conserved in the synaptotagmin gene of multiple disease vector mosquitoes. |
title_full |
Characterization of a yeast interfering RNA larvicide with a target site conserved in the synaptotagmin gene of multiple disease vector mosquitoes. |
title_fullStr |
Characterization of a yeast interfering RNA larvicide with a target site conserved in the synaptotagmin gene of multiple disease vector mosquitoes. |
title_full_unstemmed |
Characterization of a yeast interfering RNA larvicide with a target site conserved in the synaptotagmin gene of multiple disease vector mosquitoes. |
title_sort |
characterization of a yeast interfering rna larvicide with a target site conserved in the synaptotagmin gene of multiple disease vector mosquitoes. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007422 https://doaj.org/article/cbb70bccd19f4a0c9f5a983c96210642 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 5, p e0007422 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007422 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007422 https://doaj.org/article/cbb70bccd19f4a0c9f5a983c96210642 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007422 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
5 |
container_start_page |
e0007422 |
_version_ |
1766337982232526848 |