The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases
Most patients with acute suppurative meningitis are otherwise healthy individuals with regard to immune mechanisms against invasive bacterial disease. This medical emergency is among the most dramatic and potentially ravaging diseases that affect humans, particularly young children. The illness ofte...
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Instituto Oswaldo Cruz, Ministério da Saúde
2008
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ftdoajarticles:oai:doaj.org/article:cb8ca98bc22b4b25a4b29af80e645578 2023-05-15T15:08:14+02:00 The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases Mari Tuyama Renata F Boente Maria C Rebelo Ricardo P Igreja David E Barroso 2008-03-01T00:00:00Z https://doi.org/10.1590/S0074-02762008000200003 https://doaj.org/article/cb8ca98bc22b4b25a4b29af80e645578 EN eng Instituto Oswaldo Cruz, Ministério da Saúde http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000200003 https://doaj.org/toc/0074-0276 https://doaj.org/toc/1678-8060 doi:10.1590/S0074-02762008000200003 0074-0276 1678-8060 https://doaj.org/article/cb8ca98bc22b4b25a4b29af80e645578 Memórias do Instituto Oswaldo Cruz., Vol 103, Iss 2, Pp 138-142 (2008) meningitis PCR Streptococcus pneumoniae Neisseria meningitidis Haemophilus influenzae cerebrospinal fluid Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 article 2008 ftdoajarticles https://doi.org/10.1590/S0074-02762008000200003 2023-01-08T01:36:10Z Most patients with acute suppurative meningitis are otherwise healthy individuals with regard to immune mechanisms against invasive bacterial disease. This medical emergency is among the most dramatic and potentially ravaging diseases that affect humans, particularly young children. The illness often strikes suddenly, and can either result in death or leave the survivors with significant neurological dysfunctions. The demonstration of a bacterial aetiology is necessary for decisions regarding treatment and prophylaxis. Conventional bacteriological methods frequently fail to identify an agent, as a result of administration of antibiotics or delayed lumbar punctures. We investigated the major aetiologic sources of unspecified bacterial meningitis cases (G00.9, ISCD-10) by polymerase chain reaction (PCR)-based identification of Neisseria meningitidis (crgA), Streptococcus pneumoniae (ply) and Haemophilus influenzae (bexA) in cerebrospinal fluid samples. The multiplex PCR detected N. meningitidis in 92%, S. pneumoniae in 4% and H. influenzae in 1% of the 192 clinical samples assayed; 3% were negative for all three DNA targets. Bacterial DNA detection was found to be a valuable adjunct to enhance bacterial meningitis surveillance when the yield of specimens by culture is reduced. The implementation of PCR assays as a diagnostic procedure in Public Health Laboratories is perceived to be a significant advance in the investigation of bacterial meningitis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Memórias do Instituto Oswaldo Cruz 103 2 138 142 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
meningitis PCR Streptococcus pneumoniae Neisseria meningitidis Haemophilus influenzae cerebrospinal fluid Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 |
spellingShingle |
meningitis PCR Streptococcus pneumoniae Neisseria meningitidis Haemophilus influenzae cerebrospinal fluid Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 Mari Tuyama Renata F Boente Maria C Rebelo Ricardo P Igreja David E Barroso The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases |
topic_facet |
meningitis PCR Streptococcus pneumoniae Neisseria meningitidis Haemophilus influenzae cerebrospinal fluid Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 |
description |
Most patients with acute suppurative meningitis are otherwise healthy individuals with regard to immune mechanisms against invasive bacterial disease. This medical emergency is among the most dramatic and potentially ravaging diseases that affect humans, particularly young children. The illness often strikes suddenly, and can either result in death or leave the survivors with significant neurological dysfunctions. The demonstration of a bacterial aetiology is necessary for decisions regarding treatment and prophylaxis. Conventional bacteriological methods frequently fail to identify an agent, as a result of administration of antibiotics or delayed lumbar punctures. We investigated the major aetiologic sources of unspecified bacterial meningitis cases (G00.9, ISCD-10) by polymerase chain reaction (PCR)-based identification of Neisseria meningitidis (crgA), Streptococcus pneumoniae (ply) and Haemophilus influenzae (bexA) in cerebrospinal fluid samples. The multiplex PCR detected N. meningitidis in 92%, S. pneumoniae in 4% and H. influenzae in 1% of the 192 clinical samples assayed; 3% were negative for all three DNA targets. Bacterial DNA detection was found to be a valuable adjunct to enhance bacterial meningitis surveillance when the yield of specimens by culture is reduced. The implementation of PCR assays as a diagnostic procedure in Public Health Laboratories is perceived to be a significant advance in the investigation of bacterial meningitis. |
format |
Article in Journal/Newspaper |
author |
Mari Tuyama Renata F Boente Maria C Rebelo Ricardo P Igreja David E Barroso |
author_facet |
Mari Tuyama Renata F Boente Maria C Rebelo Ricardo P Igreja David E Barroso |
author_sort |
Mari Tuyama |
title |
The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases |
title_short |
The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases |
title_full |
The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases |
title_fullStr |
The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases |
title_full_unstemmed |
The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases |
title_sort |
utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2008 |
url |
https://doi.org/10.1590/S0074-02762008000200003 https://doaj.org/article/cb8ca98bc22b4b25a4b29af80e645578 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Memórias do Instituto Oswaldo Cruz., Vol 103, Iss 2, Pp 138-142 (2008) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000200003 https://doaj.org/toc/0074-0276 https://doaj.org/toc/1678-8060 doi:10.1590/S0074-02762008000200003 0074-0276 1678-8060 https://doaj.org/article/cb8ca98bc22b4b25a4b29af80e645578 |
op_doi |
https://doi.org/10.1590/S0074-02762008000200003 |
container_title |
Memórias do Instituto Oswaldo Cruz |
container_volume |
103 |
container_issue |
2 |
container_start_page |
138 |
op_container_end_page |
142 |
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1766339634504138752 |