Molecular characterization of mutations associated with resistance to second line drugs in Mycobacterium tuberculosis patients from Casablanca, Morocco
ABSTRACT The emergence and spread of extensively drug-resistant tuberculosis (XDR-TB) is a serious threat to global health. Therefore, its rapid diagnosis is crucial. The present study aimed to characterize mutations conferring resistance to second line drugs (SLDs) within multidrug Mycobacterium tu...
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2021
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ftdoajarticles:oai:doaj.org/article:cb8b8c494857445096a02489bbc844c8 2024-09-09T19:27:07+00:00 Molecular characterization of mutations associated with resistance to second line drugs in Mycobacterium tuberculosis patients from Casablanca, Morocco Ghizlane Momen Achraf Aainouss Abdelmajid Lamaammal Fouad Chettioui Mohamed Blaghen Malika Messoudi Khalid Belghmi Jamal Mouslim Mohammed El Mzibri My Driss El Messaoudi Meriem Khyatti Imane Chaoui 2021-03-01T00:00:00Z https://doi.org/10.1590/s1678-9946202163019 https://doaj.org/article/cb8b8c494857445096a02489bbc844c8 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652021000100210&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202163019 https://doaj.org/article/cb8b8c494857445096a02489bbc844c8 Revista do Instituto de Medicina Tropical de São Paulo, Vol 63 (2021) Mycobacterium tuberculosis XDR-TB DNA sequencing Drug susceptibility testing Morocco Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1590/s1678-9946202163019 2024-08-05T17:49:30Z ABSTRACT The emergence and spread of extensively drug-resistant tuberculosis (XDR-TB) is a serious threat to global health. Therefore, its rapid diagnosis is crucial. The present study aimed to characterize mutations conferring resistance to second line drugs (SLDs) within multidrug Mycobacterium tuberculosis (MDR-MTB) isolates and to estimate the occurrence of XDR-TB in Casablanca, Morocco. A panel of 200 MDR-TB isolates was collected at the Pasteur Institute between 2015-2018. Samples were subjected to drug susceptibility testing to Ofloxacin (OFX), Kanamycin (KAN) and Amikacin (AMK). The mutational status of gyrA, gyrB, rrs, tlyA and eis was assessed by sequencing these target genes. Drug susceptibility testing for SLDs showed that among the 200 MDR strains, 20% were resistant to OFX, 2.5% to KAN and 1.5% to AMK. Overall, 14.5% of MDR strains harbored mutations in gyrA, gyrB, rrs and tlyA genes. From the 40 OFXR isolates, 67.5% had mutations in QRDR of gyrA and gyrB genes, the most frequent one being Ala90Val in gyrA gene. Of note, none of the isolates harbored simultaneously mutations in gyrA and gyrB genes. In eight out of the 200 MDR-TB isolates resistant either to KAN or AMK, only 25% had A1401G or Lys89Glu change in rrs and tlyA genes respectively. This study is very informative and provides data on the alarming rate of fluoroquinolone resistance which warrants the need to implement appropriate drug regimens to prevent the emergence and spread of more severe forms of Mycobacterium tuberculosis drug resistance. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Pasteur ENVELOPE(140.099,140.099,-66.625,-66.625) Revista do Instituto de Medicina Tropical de São Paulo 63 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
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language |
English |
topic |
Mycobacterium tuberculosis XDR-TB DNA sequencing Drug susceptibility testing Morocco Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Mycobacterium tuberculosis XDR-TB DNA sequencing Drug susceptibility testing Morocco Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Ghizlane Momen Achraf Aainouss Abdelmajid Lamaammal Fouad Chettioui Mohamed Blaghen Malika Messoudi Khalid Belghmi Jamal Mouslim Mohammed El Mzibri My Driss El Messaoudi Meriem Khyatti Imane Chaoui Molecular characterization of mutations associated with resistance to second line drugs in Mycobacterium tuberculosis patients from Casablanca, Morocco |
topic_facet |
Mycobacterium tuberculosis XDR-TB DNA sequencing Drug susceptibility testing Morocco Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT The emergence and spread of extensively drug-resistant tuberculosis (XDR-TB) is a serious threat to global health. Therefore, its rapid diagnosis is crucial. The present study aimed to characterize mutations conferring resistance to second line drugs (SLDs) within multidrug Mycobacterium tuberculosis (MDR-MTB) isolates and to estimate the occurrence of XDR-TB in Casablanca, Morocco. A panel of 200 MDR-TB isolates was collected at the Pasteur Institute between 2015-2018. Samples were subjected to drug susceptibility testing to Ofloxacin (OFX), Kanamycin (KAN) and Amikacin (AMK). The mutational status of gyrA, gyrB, rrs, tlyA and eis was assessed by sequencing these target genes. Drug susceptibility testing for SLDs showed that among the 200 MDR strains, 20% were resistant to OFX, 2.5% to KAN and 1.5% to AMK. Overall, 14.5% of MDR strains harbored mutations in gyrA, gyrB, rrs and tlyA genes. From the 40 OFXR isolates, 67.5% had mutations in QRDR of gyrA and gyrB genes, the most frequent one being Ala90Val in gyrA gene. Of note, none of the isolates harbored simultaneously mutations in gyrA and gyrB genes. In eight out of the 200 MDR-TB isolates resistant either to KAN or AMK, only 25% had A1401G or Lys89Glu change in rrs and tlyA genes respectively. This study is very informative and provides data on the alarming rate of fluoroquinolone resistance which warrants the need to implement appropriate drug regimens to prevent the emergence and spread of more severe forms of Mycobacterium tuberculosis drug resistance. |
format |
Article in Journal/Newspaper |
author |
Ghizlane Momen Achraf Aainouss Abdelmajid Lamaammal Fouad Chettioui Mohamed Blaghen Malika Messoudi Khalid Belghmi Jamal Mouslim Mohammed El Mzibri My Driss El Messaoudi Meriem Khyatti Imane Chaoui |
author_facet |
Ghizlane Momen Achraf Aainouss Abdelmajid Lamaammal Fouad Chettioui Mohamed Blaghen Malika Messoudi Khalid Belghmi Jamal Mouslim Mohammed El Mzibri My Driss El Messaoudi Meriem Khyatti Imane Chaoui |
author_sort |
Ghizlane Momen |
title |
Molecular characterization of mutations associated with resistance to second line drugs in Mycobacterium tuberculosis patients from Casablanca, Morocco |
title_short |
Molecular characterization of mutations associated with resistance to second line drugs in Mycobacterium tuberculosis patients from Casablanca, Morocco |
title_full |
Molecular characterization of mutations associated with resistance to second line drugs in Mycobacterium tuberculosis patients from Casablanca, Morocco |
title_fullStr |
Molecular characterization of mutations associated with resistance to second line drugs in Mycobacterium tuberculosis patients from Casablanca, Morocco |
title_full_unstemmed |
Molecular characterization of mutations associated with resistance to second line drugs in Mycobacterium tuberculosis patients from Casablanca, Morocco |
title_sort |
molecular characterization of mutations associated with resistance to second line drugs in mycobacterium tuberculosis patients from casablanca, morocco |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2021 |
url |
https://doi.org/10.1590/s1678-9946202163019 https://doaj.org/article/cb8b8c494857445096a02489bbc844c8 |
long_lat |
ENVELOPE(140.099,140.099,-66.625,-66.625) |
geographic |
Arctic Pasteur |
geographic_facet |
Arctic Pasteur |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 63 (2021) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652021000100210&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202163019 https://doaj.org/article/cb8b8c494857445096a02489bbc844c8 |
op_doi |
https://doi.org/10.1590/s1678-9946202163019 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
63 |
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